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Recombinant human PD-L1 protein (Active) is a Human Fragment protein, in the 19 to 238 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, FuncS.
Alternative names=Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1, PDL1, B7H1, CD274, PDCD1LG1, PDCD1L1
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, HPLC, FuncS
- Biologically active
Yes
- Sequence
F T V T V P K D L Y V V E Y G S N M T I E C K F P V E K Q L D L A A L I V Y W E M E D K N I I Q F V H G E E D L K V Q H S S Y R Q R A R L L K D Q L S L G N A A L Q I T D V K L Q D A G V Y R C M I S Y G G A D Y K R I T V K V N A P Y N K I N Q R I L V V D P V T S E H E L T C Q A E G Y P K A E V I W T S S D H Q V L S G K T T T T N S K R E E K L F N V T S T L R I N T T T N E I F Y C T F R R L D P E E N H T A E L V I P E L P L A H P P N E R
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human PD-L1 protein (Active) is a Human Fragment protein, in the 19 to 238 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC, FuncS.
Alternative names=Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, hPD-L1, B7 homolog 1, B7-H1, PDL1, B7H1, CD274, PDCD1LG1, PDCD1L1
Key facts
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE, HPLC, FuncS
- Biologically active
Yes
- Biological activity
Immobilized ab167713 on Anti-His Tag (C-term) Antibody, precoated (0.1 μg/well) plate, binds Human PD-1, Mouse IgG2a Fc Tag (HPLC-verified), at 5 μg/mL (100 μL/well) with a linear range of 10-78 ng/mL.
- Predicted molecular weight
26 kDa
- Amino acids
19 to 238
- Sequence
F T V T V P K D L Y V V E Y G S N M T I E C K F P V E K Q L D L A A L I V Y W E M E D K N I I Q F V H G E E D L K V Q H S S Y R Q R A R L L K D Q L S L G N A A L Q I T D V K L Q D A G V Y R C M I S Y G G A D Y K R I T V K V N A P Y N K I N Q R I L V V D P V T S E H E L T C Q A E G Y P K A E V I W T S S D H Q V L S G K T T T T N S K R E E K L F N V T S T L R I N T T T N E I F Y C T F R R L D P E E N H T A E L V I P E L P L A H P P N E R
- Accession
- Q9NZQ7-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 5% Trehalose
Specifications
- Form
Lyophilized
- Additional notes
>90% as determined by SEC-HPLC.Purified by Immobilized metal affinity chromatography.
General info
- Function
Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).
- Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
- Post-translational modifications
Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.
- Subcellular localisation
Early endosome membrane, Recycling endosome membrane
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
For a recombinant rabbit monoclonal antibody to PD-L1 in IHC usage and KO validated, see ab205921 (clone 28-8)
For a recombinant rabbit monoclonal antibody to PD-L1 in WB and ICC/IF usage, - see ab174838 (clone EPR1161(2))
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