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AB283481

Recombinant Human PD-L2 protein (Active)

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Recombinant Human PD-L2 protein (Active) is a Human Fragment protein, in the 20 to 220 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, Biological Activity, FuncS.

View Alternative Names

CD273, B7DC, PDCD1L2, PDL2, PDCD1LG2, Programmed cell death 1 ligand 2, PD-1 ligand 2, PD-L2, PDCD1 ligand 2, Programmed death ligand 2, Butyrophilin B7-DC, B7-DC

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Mass Spectrometry - Recombinant Human PD-L2 protein (Active) (AB283481)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human PD-L2 protein (Active) (AB283481)

Mass determination by ESI-TOF.

Predicted MW is 22713.78 Da (+/- 10 Da by ESI-TOF). Observed MW is 22717.94

HPLC - Recombinant Human PD-L2 protein (Active) (AB283481)
  • HPLC

Supplier Data

HPLC - Recombinant Human PD-L2 protein (Active) (AB283481)

HPLC analysis of ab283481

SDS-PAGE - Recombinant Human PD-L2 protein (Active) (AB283481)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PD-L2 protein (Active) (AB283481)

SDS-page analysis of ab283481

Key facts

Purity

>95% HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

Mass Spec, FuncS, SDS-PAGE, HPLC, Biological Activity

applications

Biologically active

Yes

Biological activity

Fully biologically active measured by its ability to inhibit anti-CD3 and anti-CD8 antibody induced T cell expansion. ED50 is 10 μg/ml, corresponding to a specific activity of 1 X 102 units/mg.

Accession

Q9BQ51

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Biological Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"LFTVTVPKELYIIEHGSNVTLECNFDTGSHVNLGAITASLQKVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCIIIYGVAWDYKYLTLKVKASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPT","proteinLength":"Fragment","predictedMolecularWeight":"22.71 kDa","actualMolecularWeight":null,"aminoAcidEnd":220,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9BQ51","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PD-L2 also known as PDCD1LG2 or B7-DC is a member of the B7 protein family that plays a role in immune system regulation. This protein has a molecular mass of approximately 25 kDa. PD-L2 is expressed in antigen-presenting cells such as dendritic cells and macrophages. Expression is also located in some subsets of T cells and B cells. The PD-L2 can act as a second ligand for the PD-1 receptor which is important in immune checkpoints.
Biological function summary

PD-L2 offers protection to tissues from autoimmunity by regulating T-cell activation and fostering tolerance. By engaging with its receptor PD-1 PD-L2 forms a complex that modulates the immune response to prevent overactivation. This interaction limits the immune system's potential damage to healthy tissues maintaining proper immune function in diverse environments.

Pathways

PD-L2 participates in the immune checkpoint pathways where it works closely with PD-1 to mediate immune responses. PD-L2 forms an important part of the program death-1 (PD-1) pathway which plays a prominent role in downregulating T-cell activity. It relates to proteins such as PD-L1 which also interacts with PD-1 modulating immune tolerance and response to infections or tumors.

PD-L2 involvement has been significant in cancer immunology and autoimmune conditions. Its interaction with PD-1 relates to the suppression of immune responses in tumors contributing to cancer progression. PD-L2's function as an immune checkpoint ligand associates with autoimmune diseases like rheumatoid arthritis where immune regulation goes awry. Both PD-1 and PD-L2 represent potential targets for therapeutic interventions due to their roles in these conditions.
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Specifications

Form

Lyophilized

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General info

Function

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11224527, PubMed : 12538684). Acts as a ligand for the inhibitory receptor PDCD1/PD-1, inhibiting T-cell proliferation by blocking cell cycle progression and cytokine production (PubMed : 11224527).

Sequence similarities

Belongs to the immunoglobulin superfamily. BTN/MOG family.

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Product protocols

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Target data

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11224527, PubMed : 12538684). Acts as a ligand for the inhibitory receptor PDCD1/PD-1, inhibiting T-cell proliferation by blocking cell cycle progression and cytokine production (PubMed : 11224527).
See full target information PDCD1LG2
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Product promise

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