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AB128430

Recombinant Human PDCL protein (His tag N-Terminus)

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Recombinant Human PDCL protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 301 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PHLOP1, PhLP1, PDCL, Phosducin-like protein, PHLP

1 Images
SDS-PAGE - Recombinant Human PDCL protein (His tag N-Terminus) (AB128430)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human PDCL protein (His tag N-Terminus) (AB128430)

15% SDS-PAGE analysis of 3µg ab128430.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q13371

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMTTLDDKLLGEKLQYYYSSSEDEDSDHEDKDRGRCAPASSSVPAEAELAGEGISVNTGPKGVINDWRRFKQLETEQREEQCREMERLIKKLSMTCRSHLDEEEEQQKQKDLQEKISGKMTLKEFAIMNEDQDDEEFLQQYRKQRMEEMRQQLHKGPQFKQVFEISSGEGFLDMIDKEQKSIVIMVHIYEDGIPGTEAMNGCMICLAAEYPAVKFCKVKSSVIGASSQFTRNALPALLIYKGGELIGNFVRVTDQLGDDFFAVDLEAFLQEFGLLPEKEVLVLTSVRNSATCHSEDSDLEID","proteinLength":"Full Length","predictedMolecularWeight":"36.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":301,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q13371","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDCL also known as Phosducin-like protein functions as a co-chaperone that regulates G protein signaling. The protein weighs approximately 34 kDa and is expressed in various tissues including the brain and eye. PDCL interacts with other molecular chaperones to aid in the folding and assembly of G protein subunits playing an important role in maintaining cellular homeostasis.
Biological function summary

This protein plays a critical role in the modulation of phototransduction pathways in the retina. It exists as part of a complex with the G protein beta subunit influencing the regulation of vision and light response. PDCL also interacts with cytosolic chaperonin CCT highlighting its role in protein folding. Through these interactions PDCL affects signal transduction processes in neuronal tissues.

Pathways

PDCL integrates into the phototransduction and G protein-coupled receptor (GPCR) signaling pathways. Its collaboration with the beta subunit of G proteins highlights its importance in these pathways. Furthermore PDCL works alongside proteins like Gbetaγ enhancing our understanding of the regulation mechanisms in signal transduction.

PDCL shows relevance to retinitis pigmentosa and certain mood disorders. Retinitis pigmentosa is a degenerative eye disease that involves dysfunction of the phototransduction pathway where PDCL interacts with proteins involved in vision signal processing. In mood disorders PDCL's relationship with G protein signaling reveals connections to proteins influencing neurotransmitter pathways contributing to altered cellular responses.
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Specifications

Form

Liquid

Additional notes

ab128430 was purified using conventional chromatography techniques.

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General info

Function

Acts as a positive regulator of hedgehog signaling and regulates ciliary function.. Isoform 1. Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers.. Isoform 2. Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin.

Sequence similarities

Belongs to the phosducin family.

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Product protocols

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Target data

Acts as a positive regulator of hedgehog signaling and regulates ciliary function.. Isoform 1. Functions as a co-chaperone for CCT in the assembly of heterotrimeric G protein complexes, facilitates the assembly of both Gbeta-Ggamma and RGS-Gbeta5 heterodimers.. Isoform 2. Acts as a negative regulator of heterotrimeric G proteins assembly by trapping the preloaded G beta subunits inside the CCT chaperonin.
See full target information PDCL
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