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AB198450

Recombinant human PDE1C/PDE1 protein

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Recombinant human PDE1C/PDE1 protein is a Human Full Length protein, in the 2 to 634 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Cam-PDE 1C, Hcam3, PDE1C

2 Images
Functional Studies - Recombinant human PDE1C/PDE1 protein (AB198450)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PDE1C/PDE1 protein (AB198450)

Activity assay using ab198450.

SDS-PAGE - Recombinant human PDE1C/PDE1 protein (AB198450)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PDE1C/PDE1 protein (AB198450)

10% SDS-PAGE analysis of 4.41 μg ab198450 with Coomassie staining.

Key facts

Purity

>80% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥ 15,000 pmol/min/μg.

One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Assayed using a PDE1C Assay Kit. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198450 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Accession

Q14123

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PDE1C
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Sequence info

[{"sequence":"ESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAATVLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSNMVGLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALVSFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAILYNDRSVLENHHLSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKAMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVISVDYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEMEAKSQAEEGASGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQQQNGDFKDGKNKTDKKDHSNIGNDSKKTDDSQE","proteinLength":"Full Length","predictedMolecularWeight":"98.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":634,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q14123","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE1C also known as phosphodiesterase 1C is an enzyme that mechanically regulates intracellular levels of cyclic nucleotides by hydrolyzing cAMP and cGMP. It has a mass of approximately 85 kDa. You can find PDE1C expression in various tissues including the brain heart and vascular smooth muscle. This expression pattern shows its importance in diverse physiological contexts where cAMP and cGMP act as second messengers.
Biological function summary

PDE1C functions as a calcium/calmodulin-dependent phosphodiesterase integrating signals where calcium levels fluctuate. It belongs to a larger family of phosphodiesterases that help modulate intracellular signaling pathways through cyclic nucleotide metabolism. This enzyme can operate independently or as part of complexes with other proteins which allows it to finely tune cellular response to external stimuli by adjusting cAMP and cGMP concentrations.

Pathways

PDE1C plays a significant role in key biological processes related to cyclic nucleotide signaling. It is particularly important in the regulation of the cardiovascular system by affecting vasodilation and cardiac contractility through pathways involving cAMP and cGMP. The enzyme is also involved in the smooth muscle relaxation pathway. PDE1C also interacts with calcium-dependent pathways to coordinate cellular responses that involve isoforms of proteins like PKG (cGMP-dependent protein kinase) and PKA (cAMP-dependent protein kinase).

Aberrant activity of PDE1C links to conditions such as cardiovascular diseases and certain cancers. Dysregulated expression of PDE1C can alter smooth muscle function and contribute to disorders like hypertension and cardiac hypertrophy. PDE1C also interacts with proteins like VEGF (vascular endothelial growth factor) in cancer biology where it can influence tumor growth and angiogenesis by modifying cellular proliferation through altered cyclic nucleotide signaling.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Calmodulin-dependent cyclic nucleotide phosphodiesterase with a dual specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed : 29860631, PubMed : 8557689). Has a high affinity for both cAMP and cGMP (PubMed : 8557689). Modulates the amplitude and duration of the cAMP signal in sensory cilia in response to odorant stimulation, hence contributing to the generation of action potentials. Regulates smooth muscle cell proliferation. Regulates the stability of growth factor receptors, including PDGFRB (Probable).

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.

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Product protocols

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Target data

Calmodulin-dependent cyclic nucleotide phosphodiesterase with a dual specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes (PubMed : 29860631, PubMed : 8557689). Has a high affinity for both cAMP and cGMP (PubMed : 8557689). Modulates the amplitude and duration of the cAMP signal in sensory cilia in response to odorant stimulation, hence contributing to the generation of action potentials. Regulates smooth muscle cell proliferation. Regulates the stability of growth factor receptors, including PDGFRB (Probable).
See full target information PDE1C
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