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AB125603

Recombinant human PDE4A protein

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Recombinant human PDE4A protein is a Human Fragment protein, in the 332 to 886 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

DPDE2, PDE4A, PDE46, cAMP-specific phosphodiesterase 4A

2 Images
Functional Studies - Recombinant human PDE4A protein (AB125603)
  • FuncS

Unknown

Functional Studies - Recombinant human PDE4A protein (AB125603)
SDS-PAGE - Recombinant human PDE4A protein (AB125603)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PDE4A protein (AB125603)

Key facts

Purity

>95% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity was determined to be 1082 nmol/min/mg

Accession

P27815

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"110 kDa","actualMolecularWeight":null,"aminoAcidEnd":886,"aminoAcidStart":332,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P27815","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE4A or phosphodiesterase 4A is an isoform part of the PDE4 family known for hydrolyzing cAMP an important second messenger in cells. The mass of PDE4A can vary depending on the isoform with commonly referenced masses around 70-100 kDa. This enzyme is expressed in various tissues including the brain immune cells and lungs. Other popular names for this protein include PDE46 and DPDE2 indicating its different isoforms and specific functionalities across tissue types.
Biological function summary

PDE4A plays an important role in regulating intracellular levels of cyclic adenosine monophosphate (cAMP) a critical component in signaling pathways that affect numerous cellular processes. By controlling cAMP concentrations PDE4A regulates inflammatory responses and airway smooth muscle contraction which is important for respiratory functions. PDE4A does not function within a complex but operates as a homodimer when exerting its effects on signaling cascades.

Pathways

PDE4A's role is significant in the cAMP-dependent signaling pathway. This pathway influences processes like inflammation and smooth muscle relaxation. PDE4A interacts with various proteins such as PKA (protein kinase A) which is activated by cAMP and mediates several downstream effects. By influencing the cAMP pathway PDE4A affects bodily responses including immune and respiratory systems.

PDE4A has implications in conditions such as asthma and chronic obstructive pulmonary disease (COPD). Dysregulation of cAMP through PDE4A can contribute to these respiratory conditions by affecting airway inflammation and muscle tone. PDE4A is also linked to other related proteins such as PDE4B which can also impact inflammatory pathways in similar disease contexts. PDE4 inhibitors have been explored as therapeutic agents for modifying these diseases by targeting the enzymatic activity of PDE4A and related isoforms to restore balanced cAMP levels.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes.. Isoform 1. Efficiently hydrolyzes cAMP.. Isoform 2. Efficiently hydrolyzes cAMP.. Isoform 3. Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP.. Isoform 4. Efficiently hydrolyzes cAMP.. Isoform 6. Efficiently hydrolyzes cAMP.. Isoform 7. Efficiently hydrolyzes cAMP.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

Post-translational modifications

Isoform 1. Proteolytically cleaved by CASP3.. Isoform 2. Phosphorylated at Ser-119 by PKA.

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Product protocols

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Target data

Hydrolyzes the second messenger 3',5'-cyclic AMP (cAMP), which is a key regulator of many important physiological processes.. Isoform 1. Efficiently hydrolyzes cAMP.. Isoform 2. Efficiently hydrolyzes cAMP.. Isoform 3. Efficiently hydrolyzes cAMP. The phosphodiesterase activity is not affected by calcium, calmodulin or cyclic GMP (cGMP) levels. Does not hydrolyze cGMP.. Isoform 4. Efficiently hydrolyzes cAMP.. Isoform 6. Efficiently hydrolyzes cAMP.. Isoform 7. Efficiently hydrolyzes cAMP.
See full target information PDE4A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Proteins & Peptides

AB198423

Recombinant human PDE4A protein

proteins-peptides

recombinant-human-pde4a-protein-ab198423

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