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AB198456

Recombinant human PDE4D protein

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Recombinant human PDE4D protein is a Human Full Length protein, in the 2 to 507 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

DPDE3, PDE4D, PDE43, cAMP-specific phosphodiesterase 4D

2 Images
Functional Studies - Recombinant human PDE4D protein (AB198456)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PDE4D protein (AB198456)

Specific activity of ab198456.

SDS-PAGE - Recombinant human PDE4D protein (AB198456)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PDE4D protein (AB198456)

4-20% SDS-PAGE analysis of ab198456 with Coomassie staining.

Lane 1 : 2.85 μg ab198456
Lane 2 : Protein marker

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥ 13,600 pmol/min/μg
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 μM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198456 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Accession

Q08499

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"ASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT","proteinLength":"Full Length","predictedMolecularWeight":"84 kDa","actualMolecularWeight":null,"aminoAcidEnd":507,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q08499","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphodiesterase 4D (PDE4D) is an enzyme that hydrolyzes cyclic AMP (cAMP) to its inactive form AMP controlling important cellular signaling pathways. It is part of the large PDE family and sometimes referred to as DPDE1. With a molecular weight of approximately 93 kDa PDE4D is widely expressed in the brain heart lungs and immune cells. Its expression in these tissues indicates an important regulatory role in both central and peripheral physiological processes.
Biological function summary

PDE4D plays an important role in the modulation of intracellular cAMP levels impacting processes such as inflammation cognition and cardiac function. It does not operate as part of a larger complex but collaborates with other signaling molecules to influence cellular responses. PDE4D's regulation of cAMP levels directly affects protein kinase A (PKA) leading to alterations in gene expression and cell activity.

Pathways

PDE4D integrates into the cAMP signaling and inflammatory pathways impacting various cellular responses. In the cAMP signaling pathway it partners with other PDEs and signaling proteins such as PKA which phosphorylates target proteins affecting cellular function. In inflammatory pathways PDE4D modulates immune cell activity by altering the cAMP-mediated inhibition of pro-inflammatory cytokine production linking it to inflammation-related responses.

PDE4D has associations with conditions such as asthma and ischemic stroke. In asthma PDE4D regulates cAMP levels in lung cells affecting bronchial constriction and inflammation. In ischemic stroke alterations in PDE4D activity influence vascular function by changing cAMP dynamics in vascular smooth muscle cells. Within these disorders PDE4D interacts with proteins like cytokines and COX-2 which also play parts in inflammatory pathways and vascular responses.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

Post-translational modifications

Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
See full target information PDE4D
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