Recombinant human PDE4D3 protein (GST tag N-Terminus)
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Recombinant human PDE4D3 protein (GST tag N-Terminus) is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
DPDE3, PDE4D, PDE43, cAMP-specific phosphodiesterase 4D
- FuncS
Unknown
Functional Studies - Recombinant human PDE4D3 protein (GST tag N-Terminus) (AB80332)
Image showing specific activity of ab80332.
- WB
Unknown
Western blot - Recombinant human PDE4D3 protein (GST tag N-Terminus) (AB80332)
All lanes:
Western blot - Anti-PDE4D3 antibody (<a href='/en-us/products/primary-antibodies/pde4d3-antibody-ab14614'>ab14614</a>) at 1/500 dilution
All lanes:
Western blot - Recombinant human PDE4D3 protein (GST tag N-Terminus) (ab80332) at 0.01 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
Predicted band size: 76 kDa
true
Exposure time: 8min
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human PDE4D3 protein (GST tag N-Terminus) (AB80332)
10% SDS-PAGE, Coomassie staining.
Lane 1 : ab80332, 4μg.
Lane 2 : Protein marker.
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Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PDE4D3 participates in the modulation of intracellular signaling through the breakdown of cAMP. It is not part of a classical complex but closely interacts with other signaling molecules to perform its functions. This activity impacts processes such as inflammation mood regulation and airway muscle contraction. By controlling cAMP levels PDE4D3 plays a role in the appropriate response of cells to external stimuli thereby influencing cellular responses such as proliferation and differentiation.
Pathways
PDE4D3 is part of the cAMP signaling pathway a fundamental pathway impacting processes like smooth muscle relaxation and immune response regulation. It regulates the activity of protein kinase A (PKA) by controlling cAMP levels which in turn influences other proteins involved in these pathways. Another pathway involving PDE4D3 includes the mitogen-activated protein kinase (MAPK) pathway contributing to cellular responses to growth factors. PDE4D3 interacts with proteins such as β-arrestins which mediate receptor signaling and internalization linking cAMP breakdown to broad cellular effects.
Specifications
Form
Liquid
Additional notes
Affinity purified.
General info
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
Post-translational modifications
Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.
Target data
Product promise
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