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AB125786

Recombinant human PDE7A/HCP1 protein

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Recombinant human PDE7A/HCP1 protein is a Human Fragment protein, in the 104 to 456 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

View Alternative Names

HCP1, TM22, cAMP-specific phosphodiesterase 7A, PDE7A

4 Images
Functional Studies - Recombinant human PDE7A/HCP1 protein (AB125786)
  • FuncS

Unknown

Functional Studies - Recombinant human PDE7A/HCP1 protein (AB125786)

Sample assay showing the specific activity of ab125786 to be 166 nmol/min/mg.

Functional Studies - Recombinant human PDE7A/HCP1 protein (AB125786)
  • FuncS

Unknown

Functional Studies - Recombinant human PDE7A/HCP1 protein (AB125786)

The specific activity of PDE7A/HCP1 (ab125786) was determined to be 160 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant human PDE7A/HCP1 protein (AB125786)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PDE7A/HCP1 protein (AB125786)

SDS-PAGE analysis of ab125786.

SDS-PAGE - Recombinant human PDE7A/HCP1 protein (AB125786)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PDE7A/HCP1 protein (AB125786)

SDS PAGE analysis of ab125786

Key facts

Purity

>85% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab125786 was determined to be 166 nmol/min/mg.

Accession

Q13946

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.4% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PDE7A
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Sequence info

[{"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSSNSLNILDDDYNGQAKCMLEKVGNWNFDIFLFDRLTNGNSLVSLTFHLFSLHGLIEYFHLDMMKLRRFLVMIQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLANSVTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYKNTSVLENHHWRSAVGLLRESGLFSHLPLESRQQMETQIGALILATDISRQNEYLSLFRSHLDRGDLCLEDTRHRHLVLQMALKCADICNPCRTWELSKQWSEKVTEEFFHQGDIEKKYHLGVSPLCDRHTESIANIQIGFMTYLVEPLFTEWARFSNTRLSQTMLGHVGLNKASWKGLQREQSSSEDTDAAFELNSQLLPQENRLS","proteinLength":"Fragment","predictedMolecularWeight":"64 kDa","actualMolecularWeight":null,"aminoAcidEnd":456,"aminoAcidStart":104,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q13946","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE7A also known as HCP1 is a phosphodiesterase enzyme responsible for breaking down cAMP an important signaling molecule in cells. This protein has a molecular weight of approximately 52 kDa. PDE7A is expressed in various tissues including muscles and immune cells. It plays a role in regulating cellular levels of cAMP which affects various physiological processes. HCP1 and HCP1 protein are common alternate labels used to refer to PDE7A.
Biological function summary

PDE7A influences immune response and muscle functions through its enzymatic activity on cAMP. The protein does not function alone but participates within larger protein complexes that modulate cAMP signaling. Its activity can affect immune cell activation offering an important checkpoint in the immune system. Additionally PDE7A's interaction with muscle cells highlights its role in controlling muscle contraction and energy metabolism.

Pathways

PDE7A has significant roles emphasizing the cAMP and immune signaling pathways. It helps control the levels of cAMP which is important for signal transduction. PDE7A interacts with proteins like PKA (Protein Kinase A) that rely on cAMP as a secondary messenger. Through this pathway PDE7A contributes to regulating processes such as inflammatory responses and metabolic pathways interlinking with other proteins like CREB which influences gene expression.

PDE7A's role connects it to conditions such as inflammatory diseases and certain muscular disorders. Abnormal PDE7A function can lead to dysregulation in inflammation implicating it in diseases such as rheumatoid arthritis. In the context of muscular disorders PDE7A levels affect muscle function and are related to conditions such as muscle dystrophy. It shares connections with TNF-alpha a protein involved in inflammation and immune system regulation further linking PDE7A to these disorders.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 19350606, PubMed : 8389765, PubMed : 9195912). May have a role in muscle signal transduction (PubMed : 9195912).

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.

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Product protocols

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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 19350606, PubMed : 8389765, PubMed : 9195912). May have a role in muscle signal transduction (PubMed : 9195912).
See full target information PDE7A
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