Recombinant human PDE7A/HCP1 protein is a Human Fragment protein, in the 104 to 456 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S S N S L N I L D D D Y N G Q A K C M L E K V G N W N F D I F L F D R L T N G N S L V S L T F H L F S L H G L I E Y F H L D M M K L R R F L V M I Q E D Y H S Q N P Y H N A V H A A D V T Q A M H C Y L K E P K L A N S V T P W D I L L S L I A A A T H D L D H P G V N Q P F L I K T N H Y L A T L Y K N T S V L E N H H W R S A V G L L R E S G L F S H L P L E S R Q Q M E T Q I G A L I L A T D I S R Q N E Y L S L F R S H L D R G D L C L E D T R H R H L V L Q M A L K C A D I C N P C R T W E L S K Q W S E K V T E E F F H Q G D I E K K Y H L G V S P L C D R H T E S I A N I Q I G F M T Y L V E P L F T E W A R F S N T R L S Q T M L G H V G L N K A S W K G L Q R E Q S S S E D T D A A F E L N S Q L L P Q E N R L S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed:19350606, PubMed:8389765, PubMed:9195912). May have a role in muscle signal transduction (PubMed:9195912).
Alternative names
HCP1, TM22, cAMP-specific phosphodiesterase 7A, PDE7A
Recommended products
Recombinant human PDE7A/HCP1 protein is a Human Fragment protein, in the 104 to 456 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab125786 was determined to be 166 nmol/min/mg.
- Accession
- Q13946-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.4% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S S N S L N I L D D D Y N G Q A K C M L E K V G N W N F D I F L F D R L T N G N S L V S L T F H L F S L H G L I E Y F H L D M M K L R R F L V M I Q E D Y H S Q N P Y H N A V H A A D V T Q A M H C Y L K E P K L A N S V T P W D I L L S L I A A A T H D L D H P G V N Q P F L I K T N H Y L A T L Y K N T S V L E N H H W R S A V G L L R E S G L F S H L P L E S R Q Q M E T Q I G A L I L A T D I S R Q N E Y L S L F R S H L D R G D L C L E D T R H R H L V L Q M A L K C A D I C N P C R T W E L S K Q W S E K V T E E F F H Q G D I E K K Y H L G V S P L C D R H T E S I A N I Q I G F M T Y L V E P L F T E W A R F S N T R L S Q T M L G H V G L N K A S W K G L Q R E Q S S S E D T D A A F E L N S Q L L P Q E N R L S
- Accession
- Q13946
- Protein length
- Fragment
- Predicted molecular weight
- 64 kDa
- Amino acids
- 104 to 456
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed:19350606, PubMed:8389765, PubMed:9195912). May have a role in muscle signal transduction (PubMed:9195912).
- Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as PDE7A
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDE7A also known as HCP1 is a phosphodiesterase enzyme responsible for breaking down cAMP an important signaling molecule in cells. This protein has a molecular weight of approximately 52 kDa. PDE7A is expressed in various tissues including muscles and immune cells. It plays a role in regulating cellular levels of cAMP which affects various physiological processes. HCP1 and HCP1 protein are common alternate labels used to refer to PDE7A.
- Biological function summary
PDE7A influences immune response and muscle functions through its enzymatic activity on cAMP. The protein does not function alone but participates within larger protein complexes that modulate cAMP signaling. Its activity can affect immune cell activation offering an important checkpoint in the immune system. Additionally PDE7A's interaction with muscle cells highlights its role in controlling muscle contraction and energy metabolism.
- Pathways
PDE7A has significant roles emphasizing the cAMP and immune signaling pathways. It helps control the levels of cAMP which is important for signal transduction. PDE7A interacts with proteins like PKA (Protein Kinase A) that rely on cAMP as a secondary messenger. Through this pathway PDE7A contributes to regulating processes such as inflammatory responses and metabolic pathways interlinking with other proteins like CREB which influences gene expression.
- Associated diseases and disorders
PDE7A's role connects it to conditions such as inflammatory diseases and certain muscular disorders. Abnormal PDE7A function can lead to dysregulation in inflammation implicating it in diseases such as rheumatoid arthritis. In the context of muscular disorders PDE7A levels affect muscle function and are related to conditions such as muscle dystrophy. It shares connections with TNF-alpha a protein involved in inflammation and immune system regulation further linking PDE7A to these disorders.
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4 product images
Functional Studies - Recombinant human PDE7A/HCP1 protein (ab125786)
The specific activity of PDE7A/HCP1 (ab125786) was determined to be 160 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human PDE7A/HCP1 protein (ab125786)
SDS PAGE analysis of ab125786
SDS-PAGE - Recombinant human PDE7A/HCP1 protein (ab125786)
SDS-PAGE analysis of ab125786.
Functional Studies - Recombinant human PDE7A/HCP1 protein (ab125786)
Sample assay showing the specific activity of ab125786 to be 166 nmol/min/mg.
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