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AB114643

Recombinant Human PDE8A protein

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Recombinant Human PDE8A protein is a Human Fragment protein, in the 32 to 121 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

PDE8A

1 Images
SDS-PAGE - Recombinant Human PDE8A protein (AB114643)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PDE8A protein (AB114643)

12.5% SDS-PAGE image showing ab114643 Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

O60658

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"RLPQGQKTAALPRTRGAGLLESELRDGSGKKVAVADVQFGPMRFHQDQLQVLLVFTKEDNQCNGFCRACEKAGFKCTVTKEAQAVLACFL","proteinLength":"Fragment","predictedMolecularWeight":"35.53 kDa","actualMolecularWeight":null,"aminoAcidEnd":121,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"O60658","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PDE8A also known as phosphodiesterase 8A performs as an enzyme that hydrolyzes cyclic adenosine monophosphate (cAMP) to adenosine monophosphate (AMP). This action regulates the intracellular levels of cAMP an important second messenger in many signaling pathways. PDE8A has a molecular mass of approximately 100 kDa and is expressed mainly in endocrine tissues like the adrenal gland and thyroid as well as in specific immune cells.
Biological function summary

The regulatory function of PDE8A on cAMP levels impacts many processes including hormone secretion and immune responses. It often functions independently rather than as part of a complex but sometimes may interface with other proteins mediating these pathways. By controlling cAMP PDE8A influences various biological actions like glucose metabolism and T-cell activation.

Pathways

PDE8A activity connects to key signaling pathways such as the cAMP-dependent protein kinase (PKA) pathway where it modulates responses through cAMP degradation. In this pathway it interacts indirectly with proteins like CREB (cAMP response element-binding protein) affecting transcriptional activity that governs metabolic and immune responses. PDE8A also interfaces with other phosphodiesterases which further modulate the cAMP levels in the cell.

Alterations in PDE8A levels or function have links to adrenal hyperplasia where abnormal hormone production affects body processes. PDE8A and its regulatory role in cAMP balance also relate to immune function disorders as improper T-cell activation leads to autoimmune conditions. These associations illustrate PDE8A's involvement with broader protein interactions including those regulating endocrine and immune homeostasis.
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Specifications

Form

Liquid

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General info

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 18983167). May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development (PubMed : 18983167). Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling (PubMed : 23509299). Protects against cell death induced by hydrogen peroxide and staurosporine (PubMed : 23509299).

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE8 subfamily.

Post-translational modifications

Phosphorylated at Ser-359 by PKA under elevated cAMP conditions, this enhances catalytic activity.

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Product protocols

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Target data

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes (PubMed : 18983167). May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development (PubMed : 18983167). Binding to RAF1 reduces RAF1 'Ser-259' inhibitory-phosphorylation and stimulates RAF1-dependent EGF-activated ERK-signaling (PubMed : 23509299). Protects against cell death induced by hydrogen peroxide and staurosporine (PubMed : 23509299).
See full target information PDE8A
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