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AB54113

Recombinant human PDE9A protein

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Recombinant human PDE9A protein is a Human Full Length protein, expressed in Baculovirus infected Sf9 cells, with >75%, suitable for SDS-PAGE, Inhib, Phosphatase Activity.

View Alternative Names

PDE9A

Key facts

Purity

>75% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

Inhib, Phosphatase Activity, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 97 U/ug. 1 unit will release 1 pmole phosphate per minute from 400 uM 5'-GMP at 30 °C in a reaction buffer of 10 mM Tris-HCl, pH 7.4, 1 mM MgCl2. Assay buffer: 10mM Tris-HCl, pH 7.4, 1 mM Magnesium chloride ; 3', 5'-cGMP substrate: 0.4 mM; PDE9A: 5 ng/ul.

Accession

O76083

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.1535% Glutathione, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Inhib": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Phosphatase Activity": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O76083","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDE9A is a phosphodiesterase enzyme that breaks down cyclic guanosine monophosphate (cGMP) into non-cyclic GMP therefore regulating intracellular levels of cGMP. This enzyme has a molecular mass of approximately 65 kilodaltons. Researchers often refer to PDE9A also as phosphodiesterase 9A. It shows higher expression in the brain and heart with presence also noted in the kidneys and lungs.
Biological function summary

PDE9A regulates the signaling processes by controlling cGMP turnover. It functions as a part of a larger network of proteins involved in intracellular signal transduction. PDE9A does not typically form distinct complexes but interacts with signaling components to maintain balanced cGMP concentrations which is essential for signaling related to vasodilation and platelet aggregation.

Pathways

PDE9A influences both cardiovascular and central nervous system pathways. In the cardiovascular system it interacts with nitric oxide signaling which has connections to blood pressure regulation through vasodilation. Within the CNS PDE9A is involved in synaptic plasticity relating to proteins like phosphodiesterase PDE5 and PDE2 which also handle cyclic nucleotide breakdown affecting neurotransmitter signaling and learning processes.

PDE9A has connections to conditions like heart failure and Alzheimer’s disease. In heart failure PDE9A’s regulation of cGMP affects nitric oxide signaling and cardiac function. In Alzheimer’s disease its influence on synaptic plasticity through interactions with proteins like amyloid precursor protein (APP) suggests a role in cognitive decline making it a potential therapeutic target for modulating synaptic signaling and cognitive function.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific : compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP (PubMed : 18757755, PubMed : 21483814, PubMed : 9624146). Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed : 25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (By similarity).

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.

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Product protocols

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Target data

Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific : compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP (PubMed : 18757755, PubMed : 21483814, PubMed : 9624146). Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed : 25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (By similarity).
See full target information PDE9A
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