JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB179496

Recombinant human PDGF AB protein (Animal Free)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human PDGF AB protein (Animal Free) is a Human Full Length protein, expressed in Escherichia coli, with >95%, <1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

2 Images
Functional Studies - Recombinant human PDGF AB protein (Animal Free) (AB179496)
  • FuncS

Unknown

Functional Studies - Recombinant human PDGF AB protein (Animal Free) (AB179496)

Functional analysis of ab179496

SDS-PAGE - Recombinant human PDGF AB protein (Animal Free) (AB179496)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PDGF AB protein (Animal Free) (AB179496)

SDS PAGE analysis of ab179496 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.

Accession

P01127

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.06% Acetic acid

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product is produced with no animal-derived raw products, animal free equipment and animal free protocols.
style
left-column

Sequence info

[{"sequence":"Alpha chain:\r\nMSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATTSLNPDYREEDTGRPRESGKKRKRKRLKPT\r\n\r\n\r\nBeta chain:\r\nSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIGIVRKKPIFKKATVTLGDHLACKCETVAAARPVT","proteinLength":"Full Length","predictedMolecularWeight":"26.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P04085","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGF AB also known as Platelet-Derived Growth Factor AB is a heterodimeric protein with an approximate mass around 30 kDa. The PDGF AB protein plays an important role in cell proliferation and differentiation by activating its receptor PDGFR. It gets expressed in a variety of cell types including fibroblasts smooth muscle cells and endothelial cells. This growth factor influences cellular behaviors through its interactions with cells in connective tissues where it shows significant levels of expression.
Biological function summary

The PDGF AB protein promotes cellular proliferation and migration particularly in the context of wound healing and development. It forms part of a larger family of PDGF molecules that include homodimers and heterodimers and it requires specific receptor binding to exert its function. As a member of the growth factor family PDGF AB contributes to the signaling pathway that regulates tissue growth and repair processes.

Pathways

PDGF AB fits significantly into the PI3K/AKT and MAPK signaling pathways. These pathways are key in regulating cellular growth and survival and they involve interactions with important proteins like Ras and AKT. The activity of PDGF AB in these pathways affects various downstream molecular processes making it an integral component of cellular signaling networks.

PDGF AB connects to a few pathologies notably atherosclerosis and certain forms of cancer. It often associates with diseases where aberrant cell proliferation and migration occur. For instance in atherosclerosis PDGF AB interacts with proteins such as Integrins and MMPs which contribute to the remodeling of blood vessels. Similarly in oncology the dysregulation of PDGF AB can lead to malignancies with its interaction through growth-promoting pathways.
style
left-column

Specifications

Form

Lyophilized

Additional notes

Purity determined by Reducing and Non-reducing SDS-PAGE.

style
left-column

General info

Function

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed : 26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

style
left-column

Product protocols

style
left-column

Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed : 26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
See full target information PDGFB
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB184860

Human PDGF BB ELISA Kit

SimpleStep

ELISA - Human PDGF BB ELISA Kit (AB184860)

  • 5
  • 1
Primary Antibodies

AB178409

Anti-PDGF B antibody [EPR6834]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-PDGF B antibody [EPR6834] (AB178409)

  • 0
  • 0
Proteins & Peptides

AB316613

Recombinant Human PDGF AA Protein Standard

Sandwich ELISA - Recombinant Human PDGF AA Protein Standard (AB316613)

  • 0
  • 0
Proteins & Peptides

AB259410

Recombinant human TNF alpha protein (Active)

Premium Bioactive

Flow Cytometry - Recombinant human TNF alpha protein (Active) (AB259410)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com