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Recombinant human PDGF B protein (Active) is a Human Full Length protein, in the 82 to 190 aa range, expressed in HEK 293, with >95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Alternative names=Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, PDGFB, SIS, PDGF2
- Purity
>95% SDS-PAGE
- Endotoxin level
< 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
- Sequence
S L G S L T I A E P A M I A E C K T R T E V F E I S R R L I D R T N A N F L V W P P C V E V Q R C S G C C N N R N V Q C R P T Q V Q L R P V Q V R K I E I V R K K P I F K K A T V T L E D H L A C K C E T V A A A R P V T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human PDGF B protein (Active) is a Human Full Length protein, in the 82 to 190 aa range, expressed in HEK 293, with >95% purity, < 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Alternative names=Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis, PDGFB, SIS, PDGF2
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
- Biological activity
Fully biologically active compared to a standard. The ED50 is is 0.9388 ng/mL corresponding to a Specific Activity of 1.07 x 106 IU/mg.
- Predicted molecular weight
12.35 kDa
- Amino acids
82 to 190
- Sequence
S L G S L T I A E P A M I A E C K T R T E V F E I S R R L I D R T N A N F L V W P P C V E V Q R C S G C C N N R N V Q C R P T Q V Q L R P V Q V R K I E I V R K K P I F K K A T V T L E D H L A C K C E T V A A A R P V T
- Accession
- P01127-1
- Protein length
Full Length
- Animal free
Yes
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Specifications
- Form
Lyophilized
- Additional notes
Purity by HPLC >=95%.
General info
- Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity).
- Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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