Recombinant human PDGFAA + PDGFBB protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
a l p h a c h a i n : - M S I E E A V P A V C K T R T V I Y E I P R S Q V D P T S A N F L I W P P C V E V K R C T G C C N T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T T S L N P D Y R E E D T G R P R E S G K K R K R K R L K P T b e t a c h a i n : - M S L G S L T I A E P A M I A E C K T R T E V F E I S R R L I D R T N A N F L V W P P C V E V Q R C S G C C N N R N V Q C R P T Q V Q L R P V Q V R K I G I V R K K P I F K K A T V T L G D H L A C K C E T V A A A R P V T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).
Additional Targets
PDGFB
Alternative names
PDGF1, PDGFA, Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide
Recommended products
Recombinant human PDGFAA + PDGFBB protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Biological Activity: The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.
- Accession
- P04085-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.06% Acetic acid
Sequence info
Amino acid sequence
- a l p h a c h a i n : - M S I E E A V P A V C K T R T V I Y E I P R S Q V D P T S A N F L I W P P C V E V K R C T G C C N T S S V K C Q P S R V H H R S V K V A K V E Y V R K K P K L K E V Q V R L E E H L E C A C A T T S L N P D Y R E E D T G R P R E S G K K R K R K R L K P T b e t a c h a i n : - M S L G S L T I A E P A M I A E C K T R T E V F E I S R R L I D R T N A N F L V W P P C V E V Q R C S G C C N N R N V Q C R P T Q V Q L R P V Q V R K I G I V R K K P I F K K A T V T L G D H L A C K C E T V A A A R P V T
- Accession
- P01127
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity greater than 98% as determined by:Analysis by RP-HPLC.Reducing and non-reducing SDS-PAGE.
General info
- Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).
- Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The platelet-derived growth factors PDGFAA and PDGFBB are key proteins that function as homodimers or heterodimers. PDGFAA has a molecular weight of approximately 28 kDa and PDGFBB weighs about 24 kDa. Both proteins localize to various tissues including those involved in wound healing like the skin muscle and blood vessels. They bind to their respective receptor tyrosine kinases PDGF receptors α and β and initiate signaling cascades essential for cellular proliferation and differentiation.
- Biological function summary
PDGFAA and PDGFBB play significant roles in cellular processes such as growth survival and motility. These proteins often operate as part of a receptor-ligand complex that mediates signal transduction across the cell membrane. This regulatory action is vital for proper development and repair of connective tissues. The interactions between PDGFAA PDGFBB and their receptors contribute to angiogenesis and tissue remodeling highlighting their importance in both normal physiology and pathological conditions.
- Pathways
PDGFAA and PDGFBB are integral to the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. These pathways are critical for transmitting extracellular signals into the cell promoting cellular survival and division. The PDGFAA and PDGFBB-driven pathways interact with related proteins like Ras and Akt effectively managing cellular responses to external stimuli. Their involvement in these pathways highlights their importance in regulating the balance between cell growth and apoptosis.
- Associated diseases and disorders
The dysregulation of PDGFAA and PDGFBB links to pathological conditions such as atherosclerosis and cancer. In atherosclerosis PDGFBB collaborates with other growth factors like VEGF to incite smooth muscle cell proliferation and migration contributing to plaque formation. In certain types of cancers PDGFAA and PDGFBB overexpression supports tumor growth and metastasis involving protein partners within the tumor microenvironment. Understanding these pathways may help target therapies in these and related disorders.
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2 product images
Functional Studies - Recombinant human PDGFAA + PDGFBB protein (ab61903)
Functional analysis of ab61903
Western blot - Recombinant human PDGFAA + PDGFBB protein (ab61903)
ab61903 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human PDGF-AB is predicted to be a disulfide linked heterodimer with a predicted MW of 25.5 kDa.
All lanes: Western blot - Recombinant human PDGFAA + PDGFBB protein (ab61903)
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