JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB61903

Recombinant human PDGFAA + PDGFBB protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human PDGFAA + PDGFBB protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PDGF1, PDGFA, Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGF2, SIS, PDGFB, Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

2 Images
Functional Studies - Recombinant human PDGFAA + PDGFBB protein (AB61903)
  • FuncS

Unknown

Functional Studies - Recombinant human PDGFAA + PDGFBB protein (AB61903)

Functional analysis of ab61903

Western blot - Recombinant human PDGFAA + PDGFBB protein (AB61903)
  • WB

Unknown

Western blot - Recombinant human PDGFAA + PDGFBB protein (AB61903)

ab61903 used in Western Blot. Figure : 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human PDGF-AB is predicted to be a disulfide linked heterodimer with a predicted MW of 25.5 kDa.

All lanes:

Western blot - Recombinant human PDGFAA + PDGFBB protein (ab61903)

false

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Biological Activity: The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.

Accession

P04085

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.06% Acetic acid

storage-buffer

style
left-column

Complementary Products

Assay Kits

AB284616

Human PDGF AA ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human PDGF AA ELISA Kit (AB284616)

  • 0
  • 0
Primary Antibodies

AB178409

Anti-PDGF B antibody [EPR6834]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-PDGF B antibody [EPR6834] (AB178409)

  • 0
  • 0
Proteins & Peptides

AB316613

Recombinant Human PDGF AA Protein Standard

Sandwich ELISA - Recombinant Human PDGF AA Protein Standard (AB316613)

  • 0
  • 0
Proteins & Peptides

AB259410

Recombinant human TNF alpha protein (Active)

Premium Bioactive

Flow Cytometry - Recombinant human TNF alpha protein (Active) (AB259410)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
style
left-column

Sequence info

[{"sequence":"alpha chain:-MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT\r\nbeta chain:- MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIFKKATV TLGDHLACKC ETVAAARPVT","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P01127","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The platelet-derived growth factors PDGFAA and PDGFBB are key proteins that function as homodimers or heterodimers. PDGFAA has a molecular weight of approximately 28 kDa and PDGFBB weighs about 24 kDa. Both proteins localize to various tissues including those involved in wound healing like the skin muscle and blood vessels. They bind to their respective receptor tyrosine kinases PDGF receptors α and β and initiate signaling cascades essential for cellular proliferation and differentiation.
Biological function summary

PDGFAA and PDGFBB play significant roles in cellular processes such as growth survival and motility. These proteins often operate as part of a receptor-ligand complex that mediates signal transduction across the cell membrane. This regulatory action is vital for proper development and repair of connective tissues. The interactions between PDGFAA PDGFBB and their receptors contribute to angiogenesis and tissue remodeling highlighting their importance in both normal physiology and pathological conditions.

Pathways

PDGFAA and PDGFBB are integral to the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. These pathways are critical for transmitting extracellular signals into the cell promoting cellular survival and division. The PDGFAA and PDGFBB-driven pathways interact with related proteins like Ras and Akt effectively managing cellular responses to external stimuli. Their involvement in these pathways highlights their importance in regulating the balance between cell growth and apoptosis.

The dysregulation of PDGFAA and PDGFBB links to pathological conditions such as atherosclerosis and cancer. In atherosclerosis PDGFBB collaborates with other growth factors like VEGF to incite smooth muscle cell proliferation and migration contributing to plaque formation. In certain types of cancers PDGFAA and PDGFBB overexpression supports tumor growth and metastasis involving protein partners within the tumor microenvironment. Understanding these pathways may help target therapies in these and related disorders.
style
left-column

Specifications

Form

Lyophilized

Additional notes

Purity greater than 98% as determined by:Analysis by RP-HPLC.Reducing and non-reducing SDS-PAGE.

style
left-column

General info

Function

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

style
left-column

Product protocols

style
left-column

Target data

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB (By similarity).
See full target information PDGFA

Additional targets

PDGFB
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com