Recombinant Human PDGFC protein is a Human Full Length protein, in the 1 to 345 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M S L F G L L L L T S A L A G Q R Q G T Q A E S N L S S K F Q F S S N K E Q N G V Q D P Q H E R I I T V S T N G S I H S P R F P H T Y P R N T V L V W R L V A V E E N V W I Q L T F D E R F G L E D P E D D I C K Y D F V E V E E P S D G T I L G R W C G S G T V P G K Q I S K G N Q I R I R F V S D E Y F P S E P G F C I H Y N I V M P Q F T E A V S P S V L P P S A L P L D L L N N A I T A F S T L E D L I R Y L E P E R W Q L D L E D L Y R P T W Q L L G K A F V F G R K S R V V D L N L L T E E V R L Y S C T P R N F S V S I R E E L K R T D T I F W P G C L L V K R C G G N C A C C L H N C N E C Q C V P S K V T K K Y H E V L Q L R P K T G V R G L H K S L T D V A L E H H E E C D C V C R G S T G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.
Alternative names
SCDGF, UNQ174/PRO200, PDGFC, Platelet-derived growth factor C, PDGF-C, Fallotein, Spinal cord-derived growth factor, VEGF-E
Recommended products
Recombinant Human PDGFC protein is a Human Full Length protein, in the 1 to 345 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q9NRA1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M S L F G L L L L T S A L A G Q R Q G T Q A E S N L S S K F Q F S S N K E Q N G V Q D P Q H E R I I T V S T N G S I H S P R F P H T Y P R N T V L V W R L V A V E E N V W I Q L T F D E R F G L E D P E D D I C K Y D F V E V E E P S D G T I L G R W C G S G T V P G K Q I S K G N Q I R I R F V S D E Y F P S E P G F C I H Y N I V M P Q F T E A V S P S V L P P S A L P L D L L N N A I T A F S T L E D L I R Y L E P E R W Q L D L E D L Y R P T W Q L L G K A F V F G R K S R V V D L N L L T E E V R L Y S C T P R N F S V S I R E E L K R T D T I F W P G C L L V K R C G G N C A C C L H N C N E C Q C V P S K V T K K Y H E V L Q L R P K T G V R G L H K S L T D V A L E H H E E C D C V C R G S T G G
- Accession
- Q9NRA1
- Protein length
- Full Length
- Predicted molecular weight
- 65.4 kDa
- Amino acids
- 1 to 345
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function.
- Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
- Post-translational modifications
Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after basic residues in the hinge region (region connecting the CUB and growth factor domains) gives rise to the receptor-binding form. Cleaved by PLAT and PLG.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PDGFC also called Platelet-Derived Growth Factor C functions mechanically as a growth factor involved in cell proliferation migration and survival. It belongs to the PDGF family and exhibits a molecular mass of approximately 38 kDa. PDGFC expresses in a variety of tissues such as the heart kidney and skeletal muscle. Unlike other PDGF family members PDGFC has a unique hinge region that requires proteolytic activation to reveal its growth factor activity.
- Biological function summary
PDGFC supports angiogenesis and tissue remodeling which are essential for normal developmental processes and wound healing. It often forms part of a PDGF receptor-ligand complex by binding to the alpha and beta PDGF receptors. Upon binding PDGFC activates signaling pathways that result in cellular responses significant in tissues undergoing repair or in presence of injury.
- Pathways
PDGFC plays a part in the MAPK and PI3K/AKT signaling pathways which regulate several cellular mechanisms. As it interacts with PDGF receptors PDGFC can collaborate with other proteins such as PDGFA and PDGFB both of which are significant for initiating similar pathways. These connections allow PDGFC to influence processes like cell cycle progression and apoptosis key elements in maintaining cellular homeostasis.
- Associated diseases and disorders
Alterations in PDGFC expression or signaling link to certain conditions such as cancer and fibrotic diseases. Overexpression of PDGFC relates to tumor growth and metastasis partially due to its role in angiogenesis where it can interact with proteins like VEGF. In fibrosis PDGFC acts by promoting excessive extracellular matrix production further involving it with proteins like transforming growth factor-beta (TGF-β) which contribute to tissue scarring and organ dysfunction.
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1 product image
SDS-PAGE - Recombinant Human PDGFC protein (ab132727)
12.5% SDS-PAGE analysis of ab132727 stained with Coomassie Blue.
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