JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB202213

Recombinant Human PDHX protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human PDHX protein (His tag N-Terminus) is a Human Full Length protein, in the 54 to 501 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

PDX1, PDHX, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex, E3-binding protein, Lipoyl-containing pyruvate dehydrogenase complex component X, proX, E3BP

1 Images
SDS-PAGE - Recombinant Human PDHX protein (His tag N-Terminus) (AB202213)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PDHX protein (His tag N-Terminus) (AB202213)

15% SDS-PAGE analysis of 3 μg ab202213

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O00330

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATDKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA","proteinLength":"Full Length","predictedMolecularWeight":"50.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":501,"aminoAcidStart":54,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00330","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDHX also known as pyruvate dehydrogenase protein X is a part of the pyruvate dehydrogenase complex. It has a molecular mass of about 54 kDa. PDHX is expressed mostly in tissues with high energy demands such as the heart and skeletal muscle. The protein has a critical role in linking glycolysis and the Krebs cycle by transferring the lipoamide group in the pyruvate dehydrogenase complex.
Biological function summary

PDHX functions as a component of the multi-enzyme pyruvate dehydrogenase complex (PDC). This complex is essential for the oxidative decarboxylation of pyruvate to acetyl-CoA within the mitochondria. PDHX coordinates with other enzymes such as E1 E2 and E3 carrying out its function by acting as an anchor to stabilize the PDC enhancing the efficiency of the catalytic process.

Pathways

PDHX participates in important metabolic pathways like glycolysis and the Krebs cycle. It relates closely with proteins such as E1 (pyruvate dehydrogenase) and E2 (dihydrolipoamide acetyltransferase) due to its role in facilitating the transition of energy substrates into the cycle. Its activity helps in the regulation of energy production within cells and impacts processes that depend on efficient energy conversion.

Mutations or deficiencies in PDHX have connections with pyruvate dehydrogenase deficiency and metabolic disorders like Leigh syndrome. These conditions affect energy metabolism and can lead to severe neurological problems. The malfunction of PDHX impacts the pyruvate dehydrogenase complex as a whole leading to an increase of pyruvate levels and a decrease in ATP production linking it also with other involved proteins like E1 and E2.
style
left-column

Specifications

Form

Liquid

Additional notes

ab202213 was purified using conventional chromatography.

style
left-column

General info

Function

Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.

Sequence similarities

Belongs to the 2-oxoacid dehydrogenase family.

Post-translational modifications

Delipoylated at Lys-97 by SIRT4, delipoylation decreases the PHD complex activity.

Subcellular localisation

Mitochondrion matrix

style
left-column

Product protocols

style
left-column

Target data

Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.
See full target information PDHX
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB219207

Anti-PDX1 antibody [EPR22002]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDX1 antibody [EPR22002] (AB219207)

  • 0
  • 0
Primary Antibodies

AB110333

Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5]

KO Validated

Western blot - Anti-Pyruvate dehydrogenase E2/E3bp antibody [13G2AE2BH5] (AB110333)

  • 4
  • 6
Primary Antibodies

AB181547

Anti-Insulin antibody [EPR17359]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Insulin antibody [EPR17359] (AB181547)

  • 5
  • 11
Primary Antibodies

AB221549

Anti-nkx6.1 antibody [EPR20405]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (AB221549)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com