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AB152020

Recombinant Human PDIA6 protein

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Recombinant Human PDIA6 protein is a Human Full Length protein, in the 20 to 440 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

ERP5, P5, TXNDC7, PDIA6, Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, ER protein 5, ERp5

Key facts

Purity

>95% SDS-PAGE

Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. ab152020 was 0.2 µM filtered.

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Primary Antibodies

AB154820

Anti-PDIA6 antibody [EPR10132(B)]

RabMAb|Recombinant|KO Validated

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Proteins & Peptides

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"LYSSSDDVIELTPSNFNREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKKDVIELTDDSFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDDLGKDELVDHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"47.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":440,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q15084","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDIA6 also known as Protein Disulfide Isomerase Family A Member 6 is a protein with a molecular mass of approximately 48 kDa. This protein resides primarily in the endoplasmic reticulum (ER) where it engages in the formation and rearrangement of disulfide bonds during the protein folding process. PDIA6 also facilitates chaperone activity aiding the proper folding of nascent proteins. Its expression is widespread but shows higher levels in secretory tissues reflecting its involvement in protein synthesis and processing.
Biological function summary

PDIA6 plays a critical role in maintaining cellular homeostasis by ensuring correct protein folding within the ER. It interacts with various substrates and is part of a larger protein complex along with other ER-resident chaperones and foldases. This protein also responds to ER stress by assisting in the unfolded protein response (UPR) a signaling cascade that restores normal function of the ER or triggers apoptosis if the stress is unresolved.

Pathways

PDIA6 holds a significant position in the folding modification and degradation of proteins via the ER-associated degradation (ERAD) pathway. This pathway involves collaboration with proteins such as BiP and ERp72 which together prevent accumulation of misfolded proteins. PDIA6 also contributes to the cellular redox homeostasis playing an important role in maintaining the balance of oxidative states within the cell which can influence pathways like the UPR.

PDIA6 associates with conditions characterized by ER stress including neurodegenerative disorders like Alzheimer's disease as well as cancer. In Alzheimer’s disease alterations in its function may affect the handling of misfolded proteins aligning it with amyloid precursor protein processing. In cancer its modulation of protein folding and stress responses links it to oncogenic pathways interacting with proteins such as p53 which can influence cell apoptosis and survival.
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General info

Function

May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed : 12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed : 24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed : 24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed : 15466936).

Sequence similarities

Belongs to the protein disulfide isomerase family.

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Product protocols

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Target data

May function as a chaperone that inhibits aggregation of misfolded proteins (PubMed : 12204115). Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling (PubMed : 24508390). May also regulate the UPR via the EIF2AK3 UPR sensor (PubMed : 24508390). Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin (PubMed : 15466936).
See full target information PDIA6
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Product promise

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