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AB152027

Recombinant Human PDILT protein

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Recombinant Human PDILT protein is a Human Full Length protein, in the 21 to 584 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

Protein disulfide-isomerase-like protein of the testis, PDILT

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, HPLC

applications

Biologically active

No

Accession

Q8N807

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"SPEVNAGVSSIHITKPVHILEERSLLVLTPAGLTQMLNQTRFLMVLFHNPSSKQSRNLAEELGKAVEIMGKGKNGIGFGKVDITIEKELQQEFGITKAPELKLFFEGNRSEPISCKGVVESAALVVWLRRQISQKAFLFNSSEQVAEFVISRPLVIVGFFQDLEEEVAELFYDVIKDFPELTFGVITIGNVIGRFHVTLDSVLVFKKGKIVNRQKLINDSTNKQELNRVIKQHLTDFVIEYNTENKDLISELHIMSHMLLFVSKSSESYGIIIQHYKLASKEFQNKILFILVDADEPRNGRVFKYFRVTEVDIPSVQILNLSSDARYKMPSDDITYESLKKFGRSFLSKNATKHQSSEEIPKYWDQGLVKQLVGKNFNVVVFDKEKDVFVMFYAPWSKKCKMLFPLLEELGRKYQNHSTIIIAKIDVTANDIQLMYLDRYPFFRLFPSGSQQAVLYKGEHTLKGFSDFLESHIKTKIEDEDELLSVEQNEVIEEEVLAEEKEVPMMRKGLPEQQSPELENMTKYVSKLEEPAGKKKTSEEVVVVVAKPKGPPVQKKKPKVKEELVDHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"65.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":584,"aminoAcidStart":21,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q8N807","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDILT also known as Protein Disulfide Isomerase-Like Testis Expressed is a member of the protein disulfide isomerase (PDI) family. This protein has a molecular mass of approximately 70 kDa and a unique structure. PDILT lacks one of the two active CGHC motifs usually seen in this family while it possesses the ER-retention signal KDEL. This protein is expressed mainly in the testis with a strong association to male germ cells. Its expression links to the endoplasmic reticulum suggesting a role in protein folding and quality control.
Biological function summary

PDILT plays an essential role in ensuring proper sperm development. Although it is not formally part of a larger protein complex it interacts with other proteins in the testis to facilitate the assembly of protein disulfide bonds. These interactions ensure the correct formation of complex disulfide-bonded proteins which are necessary for sperm structure and function. PDILT is involved in managing oxidative stress within the cells during the process of spermatogenesis.

Pathways

PDILT plays an important part in the oxidative protein folding pathway within the endoplasmic reticulum. This pathway is critical for forming disulfide bonds in proteins a process important for their stability and function. PDILT has connections with proteins like Calnexin an important component of the quality control pathway in the ER. Its functional synergy with other PDI family members highlights its role in maintaining the balance of protein homeostasis during reproduction.

PDILT has shown links to male infertility particularly regarding improper sperm motility or structure. Its malfunction can lead to a disruption in the formation of sperm disulfide bonds resulting in structural abnormalities. This dysfunction associates with other proteins like Calreticulin which is also involved in proper protein folding. Current research continues to investigate PDILT's broader implications in fertility emphasizing its significance in maintaining reproductive health.
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Specifications

Form

Liquid

Additional notes

Purity is greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. ab152027 is 0.2 µM filtered.

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General info

Function

Probable redox-inactive chaperone involved in spermatogenesis.

Sequence similarities

Belongs to the protein disulfide isomerase family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Probable redox-inactive chaperone involved in spermatogenesis.
See full target information PDILT
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