Recombinant human PDK1 protein
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Recombinant human PDK1 protein is a Human Full Length protein, in the 1 to 436 aa range, expressed in Baculovirus infected Sf9 cells, with 95%, suitable for SDS-PAGE, WB, FuncS.
View Alternative Names
PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1
- FuncS
Unknown
Functional Studies - Recombinant human PDK1 protein (AB125580)
The specific activity of PDK1 (ab125580) was determined to be 1.4 nmol/min/mg as per activity assay protocol and was equivalent to 1.9 nmol/min/mg as per radiometric assay
- FuncS
Unknown
Functional Studies - Recombinant human PDK1 protein (AB125580)
The specific activity of ab125580 was determined to be 1.7 nmol/min/mg.
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human PDK1 protein (AB125580)
SDS Page analysis of ab125580
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant human PDK1 protein (AB125580)
SDS PAGE analysis of ab125580
Complementary Products
Reagents, Controls & Accessories
AB325995
Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)
- 0
- 0
Reactivity data
Product details
ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity
This product was previously labelled as Mitochondrial Pyruvate dehydrogenase kinase 1
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
Additional notes
The purity was determined to be 95% by densitometry. Affinity purified.
General info
Function
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2 (PubMed : 7499431, PubMed : 18541534, PubMed : 22195962, PubMed : 26942675, PubMed : 17683942). This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675). Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675).
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Post-translational modifications
Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect (PubMed:22195962). Phosphorylated under hypoxic conditions at Thr-338 by phosphoglycerate kinase PGK1 which has an activating effect (PubMed:26942675).
Target data
Product promise
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