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AB271688

Recombinant Human PDK1 protein (GST tag N-Terminus)

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Recombinant Human PDK1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 2 to 436 aa range, expressed in Baculovirus infected Sf9 cells, with >15%, suitable for SDS-PAGE.

View Alternative Names

PDHK1, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1

1 Images
SDS-PAGE - Recombinant Human PDK1 protein (GST tag N-Terminus) (AB271688)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PDK1 protein (GST tag N-Terminus) (AB271688)

SDS-PAGE analysis of 1 μg ab271688.

Key facts

Purity

>15% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q15118

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.4% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.05% Sorbitan monolaurate, ethoxylated

storage-buffer

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Complementary Products

Primary Antibodies

AB202468

Anti-PDK1 antibody [EPR19571]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PDK1 antibody [EPR19571] (AB202468)

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Proteins & Peptides

AB125602

Recombinant Human PDHA1 protein

SDS-PAGE - Recombinant Human PDHA1 protein (AB125602)

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Primary Antibodies

AB177461

Anti-PDHA1 (phospho S293) antibody [EPR12200]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDHA1 (phospho S293) antibody [EPR12200] (AB177461)

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Proteins & Peptides

AB60834

Recombinant human PDPK1 protein

Functional Studies - Recombinant human PDPK1 protein (AB60834)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"RLARLLRGAALAGPGPGLRAAGFSRSFSSDSGSSPASERGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKAIYDFTDTVIRIRNRHNDVIPTMAQGVIEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGKGSPSHRKHIGSINPNCNVLEVIKDGYENARRLCDLYYINSPELELEELNAKSPGQPIQVVYVPSHLYHMVFELFKNAMRATMEHHANRGVYPPIQVHVTLGNEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTDSIERLPVYNKAAWKHYNTNHEADDWCVPSREPKDMTTFRSA","proteinLength":"Full Length","predictedMolecularWeight":"71.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":436,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q15118","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2 (PubMed : 7499431, PubMed : 18541534, PubMed : 22195962, PubMed : 26942675, PubMed : 17683942). This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675). Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675).

Sequence similarities

Belongs to the PDK/BCKDK protein kinase family.

Post-translational modifications

Phosphorylated by constitutively activated ABL1, FGFR1, FLT3 and JAK2 (in vitro), and this may also occur in cancer cells that express constitutively activated ABL1, FGFR1, FLT3 and JAK2. Phosphorylation at Tyr-243 and Tyr-244 strongly increases kinase activity, while phosphorylation at Tyr-136 has a lesser effect (PubMed:22195962). Phosphorylated under hypoxic conditions at Thr-338 by phosphoglycerate kinase PGK1 which has an activating effect (PubMed:26942675).

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2 (PubMed : 7499431, PubMed : 18541534, PubMed : 22195962, PubMed : 26942675, PubMed : 17683942). This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675). Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia (PubMed : 18541534, PubMed : 22195962, PubMed : 26942675).
See full target information PDK1
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