Recombinant human PDK3 protein is a Human Full Length protein, in the 1 to 406 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.
Alternative names
PDHK3, PDK3, Pyruvate dehydrogenase kinase isoform 3
Recommended products
Recombinant human PDK3 protein is a Human Full Length protein, in the 1 to 406 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab125606 was determined to be 12 nmol/mg/min.
- Accession
- Q15120-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- Accession
- Q15120
- Protein length
- Full Length
- Predicted molecular weight
- 69 kDa
- Amino acids
- 1 to 406
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purity determined to be >90% by densitometry. Affinity purified.Purity is lot specific. Please contact our technical Support team for details.
General info
- Function
Inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. Can also phosphorylate PDHA2. Decreases glucose utilization and increases fat metabolism in response to prolonged fasting, and as adaptation to a high-fat diet. Plays a role in glucose homeostasis and in maintaining normal blood glucose levels in function of nutrient levels and under starvation. Plays a role in the generation of reactive oxygen species.
- Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Recombinant Human PDHA1 protein ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDK3 also known as Pyruvate Dehydrogenase Kinase Isozyme 3 plays a mechanical role in regulating the activity of the pyruvate dehydrogenase (PDH) complex by phosphorylation. It phosphorylates the E1 component of the PDH complex which inhibits PDH activity and reduces the conversion of pyruvate to acetyl-CoA. PDK3 has a molecular mass of approximately 47 kDa and is mainly expressed in skeletal muscle and heart tissues.
- Biological function summary
This kinase functions as an important modulator of glucose metabolism. PDK3 acts within the mitochondrial matrix interacting with its substrate as part of the larger PDH complex. Through its regulatory influence PDK3 helps maintain cellular energy homeostasis by balancing usage of carbohydrates and lipids depending on metabolic demands and availability of nutrients.
- Pathways
PDK3 integrates into critical energy metabolism networks such as the tricarboxylic acid (TCA) cycle and the gluconeogenesis pathway. It regulates the PDH complex by inhibition impacting the flow of carbon through these pathways. PDK3's activity relates closely with other PDK isoforms such as PDK1 PDK2 and PDK4 which also modulate PDH complex function depending on tissue-specific and metabolic conditions.
- Associated diseases and disorders
PDK3 holds significance in diabetes and cancer. In type 2 diabetes PDK3's inhibition of PDH can contribute to impaired glucose oxidation leading to elevated blood glucose levels. In cancer the upregulation of PDK3 may support the metabolic switch to aerobic glycolysis a hallmark of many tumors commonly known as the Warburg effect. The activity of PDK3 in these conditions connects with lactate dehydrogenase (LDH) in cancer as both enzymes participate in metabolic reprogramming favoring lactate production.
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4 product images
Functional Studies - Recombinant human PDK3 protein (ab125606)
The specific activity of PDK3 (ab125606) was determined to be 11 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human PDK3 protein (ab125606)
SDS PAGE analysis of ab125606
Functional Studies - Recombinant human PDK3 protein (ab125606)
The specific activity of ab125606 was determined to be 12 nmol/mg/min by Kinase Assay.
SDS-PAGE - Recombinant human PDK3 protein (ab125606)
SDS-PAGE analysis of ab125606.
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