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AB125560

Recombinant human PDK4 protein

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(1 Publication)

Recombinant human PDK4 protein is a Human Full Length protein, in the 1 to 411 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

PDHK4, PDK4, Pyruvate dehydrogenase kinase isoform 4

2 Images
Functional Studies - Recombinant human PDK4 protein (AB125560)
  • FuncS

Unknown

Functional Studies - Recombinant human PDK4 protein (AB125560)

The specific activity of ab125560 was determined to be 20 nmol/min/mg.

SDS-PAGE - Recombinant human PDK4 protein (AB125560)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PDK4 protein (AB125560)

SDS PAGE analysis of ab125560 (PDK4 protein).

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, WB, SDS-PAGE

applications

Biologically active

Yes

Biological activity

The specific activity of ab125560 is 20 nmol/min/mg.

Accession

Q16654

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

  • 0
  • 0
Primary Antibodies

AB110336

Anti-PDK4 antibody [1C2BG5]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PDK4 antibody [1C2BG5] (AB110336)

  • 3
  • 1
Assay Kits

AB109902

Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit

Functional Studies - Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (AB109902)

  • 3
  • 4
Proteins & Peptides

AB125592

Recombinant human PDK2 protein

Functional Studies - Recombinant human PDK2 protein (AB125592)

  • 0
  • 0
Proteins & Peptides

AB125580

Recombinant human PDK1 protein

Functional Studies - Recombinant human PDK1 protein (AB125580)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab125602 (Human Pyruvate Dehydrogenase E1-alpha subunit full length protein) can be utilized as a substrate for assessing Kinase activity
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"71 kDa","actualMolecularWeight":null,"aminoAcidEnd":411,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q16654","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDK4 also known as Pyruvate Dehydrogenase Kinase Isozyme 4 plays an essential role in regulating mitochondrial energy metabolism. PDK4 phosphorylates the pyruvate dehydrogenase complex controlling the transformation of pyruvate to acetyl-CoA a critical step in energy production. The protein has a molecular weight of around 49 kDa. PDK4 expression occurs in various tissues with high levels observed in skeletal muscle heart and liver reflecting its role in energy metabolic processes.
Biological function summary

The enzyme functions to inhibit the pyruvate dehydrogenase complex by adding phosphate groups which reduces the conversion of pyruvate to acetyl-CoA and shifts energy production from carbohydrates to fats especially during fasting or prolonged exercise. PDK4 operates as a homodimer and is often part of a larger regulatory system balancing glucose and fatty acid oxidation in response to nutrient availability.

Pathways

PDK4 is integral to the regulation of both glucose metabolism and fatty acid oxidation. The enzyme interacts closely with the insulin signaling pathway where it modulates glucose uptake and utilization based on hormonal signals. Additionally PDK4 is a part of the hypoxia-inducible factor-1 (HIF-1) pathway where it plays a role in cellular response to low oxygen levels interacting with related proteins such as pyruvate dehydrogenase (PDH).

PDK4's activity is linked to metabolic conditions like diabetes and obesity where its altered expression can lead to insulin resistance by affecting glucose utilization. Furthermore PDK4 is associated with cardiovascular diseases through its influence on cardiac energy metabolism and its interaction with other proteins such as PDH contributing to heart dysfunction when deregulated.
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Specifications

Form

Liquid

Additional notes

ab125560 is >90% pure.

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General info

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.

Sequence similarities

Belongs to the PDK/BCKDK protein kinase family.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
See full target information PDK4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23215 PubMed37737961

2023

PDK4 facilitates fibroblast functions and diabetic wound healing through regulation of HIF-1α protein stability and gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhouji Ma,Ran Mo,Ping Yang,Youjun Ding,Hao Zhang,Zheng Dong,Yutong Chen,Qian Tan
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