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AB177676

Recombinant Human PDLIM1/CLP36 protein

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(1 Publication)

Recombinant Human PDLIM1/CLP36 protein is a Human Full Length protein, in the 1 to 329 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CLIM1, CLP36, PDLIM1, PDZ and LIM domain protein 1, C-terminal LIM domain protein 1, Elfin, LIM domain protein CLP-36

1 Images
SDS-PAGE - Recombinant Human PDLIM1/CLP36 protein (AB177676)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PDLIM1/CLP36 protein (AB177676)

15% SDS-PAGE analysis of ab177676 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O00151

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.06% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PDLIM1
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSEFMTTQQIDLQGPGPWGFRLVGGKDFEQPLAISRVTPGSKAALANLCIGDVITAIDGENTSNMTHLEAQNRIKGCTDNLTLTVARSEHKVWSPLVTEEGKRHPYKMNLASEPQEVLHIGSAHNRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLDHAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFFVEDQIYCEKHARERVTPPEGYEVVTVFPK","proteinLength":"Full Length","predictedMolecularWeight":"38.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":329,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00151","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDLIM1 also known as CLP36 is a protein that plays a mechanical role as an actin-binding protein. It is part of the PDZ-LIM family of proteins characterized by the presence of PDZ and LIM domains. PDLIM1 has a molecular mass of approximately 36 kDa and is involved in cytoskeletal organization by connecting actin filaments to the cross-linked network. It expresses mostly in tissues like the heart skeletal muscles and to some degree in others such as liver and lungs.
Biological function summary

PDLIM1 connects to actin filaments helping in maintaining cytoskeletal dynamics and cell structure. This protein is part of the larger complex involved in organizing actin filaments. PDLIM1 through interactions with cytoskeletal components regulates muscle contraction and cell motility. It stabilizes actin filaments through its PDZ domain and modulates signal transduction pathways linked to cellular adhesion and movement.

Pathways

Studies have identified PDLIM1's involvement in the regulation of signaling pathways such as the MAPK and Wnt pathways. Through these processes PDLIM1 interacts with proteins like kinases and phosphatases which play a role in signal transduction and cellular communication. By modulating these pathways PDLIM1 influences cell growth and differentiation processes critical in tissue development and maintenance.

PDLIM1 is linked to conditions involving muscle malfunction and some cancer forms. Abnormal expression of PDLIM1 correlates with cardiomyopathies as it affects heart muscle function. Also changes in PDLIM1 levels have connections to several cancers possibly through pathways involving proteins such as β-catenin an important player in cell proliferation and tumor growth. Understanding these links may provide insights into novel therapeutic strategies for these conditions.
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Specifications

Form

Liquid

Additional notes

ab177676 is purified using conventional chromatography techniques.

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General info

Function

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed : 10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed : 10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).
See full target information PDLIM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 13:872253 PubMed35547257

2022

Evaluating a Panel of Autoantibodies Against Tumor-Associated Antigens in Human Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Manli Luo,Songmei Wu,Yan Ma,Hong Liang,Yage Luo,Wentao Gu,Lijuan Fan,Yang Hao,Haiting Li,Linbo Xing
View all publications
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Complementary Products

Primary Antibodies

AB129015

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Flow Cytometry (Intracellular) - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (AB129015)

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Primary Antibodies

AB305269

Alexa Fluor® 488 Anti-HA tag antibody [EPR22819-101]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-HA tag antibody [EPR22819-101] (AB305269)

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AB174560

Recombinant Human KCTD4 protein

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Product promise

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