Recombinant human PDPK1 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS, WB.
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- Nature cell biology 24:1099-11132022A p53-phosphoinositide signalosome regulates nuclear AKT activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMo Chen,Suyong Choi,Tianmu Wen,Changliang Chen,Narendra Thapa,Jeong Hyo Lee,Vincent L Cryns,Richard A AndersonPubMed 35798843
- Nature communications 12:60352021Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessandra Stangherlin,Joseph L Watson,David C S Wong,Silvia Barbiero,Aiwei Zeng,Estere Seinkmane,Sew Peak Chew,Andrew D Beale,Edward A Hayter,Alina Guna,Alison J Inglis,Marrit Putker,Eline Bartolami,Stefan Matile,Nicolas Lequeux,Thomas Pons,Jason Day,Gerben van Ooijen,Rebecca M Voorhees,David A Bechtold,Emmanuel Derivery,Rachel S Edgar,Peter Newham,John S O'NeillPubMed 34654800
- Food & function 10:592-6012019Cyanidin inhibits EMT induced by oxaliplatin via targeting the PDK1-PI3K/Akt signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Li,Ze-Sheng Zhang,Xiao-Han Zhang,Sheng-Nan Yang,Dong Liu,Cui-Ru Diao,Hao Wang,Fu-Ping ZhengPubMed 30672917
Key facts
- Purity
- >80% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab60834 can be used as a WB positive control in conjunction with ab31406. |
Target data
Function
Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases (PubMed:10226025, PubMed:10480933, PubMed:10995762, PubMed:12167717, PubMed:14585963, PubMed:14604990, PubMed:16207722, PubMed:16251192, PubMed:17327236, PubMed:17371830, PubMed:18835241, PubMed:9094314, PubMed:9368760, PubMed:9445476, PubMed:9445477, PubMed:9707564, PubMed:9768361). Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), TSSK3, protein kinase PKN (PKN1 and PKN2) (PubMed:10226025, PubMed:10480933, PubMed:10995762, PubMed:12167717, PubMed:14585963, PubMed:14604990, PubMed:16207722, PubMed:16251192, PubMed:17327236, PubMed:17371830, PubMed:18835241, PubMed:9094314, PubMed:9368760, PubMed:9445476, PubMed:9707564, PubMed:9768361). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage (PubMed:10226025, PubMed:12167717, PubMed:9094314). Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). Activates PPARG transcriptional activity and promotes adipocyte differentiation (By similarity). Activates the NF-kappa-B pathway via phosphorylation of IKKB (PubMed:16207722). The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II (PubMed:14585963). Controls proliferation, survival, and growth of developing pancreatic cells (By similarity). Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells (By similarity). Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis (PubMed:17371830). Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response (By similarity). Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses (By similarity). Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages (By similarity). Isoform 3. Catalytically inactive.
Alternative names
PDK1, PDPK1, 3-phosphoinositide-dependent protein kinase 1, hPDK1
Recommended products
Recombinant human PDPK1 protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >80% purity and suitable for SDS-PAGE, FuncS, WB.
Key facts
- Purity
- >80% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, WB
- Biologically active
- Yes
- Biological activity
- Specific Activity: 123 nmol/min/mg.
- Accession
- O15530-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7
Preservative: 1.02% Imidazole
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.00308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases (PubMed:10226025, PubMed:10480933, PubMed:10995762, PubMed:12167717, PubMed:14585963, PubMed:14604990, PubMed:16207722, PubMed:16251192, PubMed:17327236, PubMed:17371830, PubMed:18835241, PubMed:9094314, PubMed:9368760, PubMed:9445476, PubMed:9445477, PubMed:9707564, PubMed:9768361). Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), TSSK3, protein kinase PKN (PKN1 and PKN2) (PubMed:10226025, PubMed:10480933, PubMed:10995762, PubMed:12167717, PubMed:14585963, PubMed:14604990, PubMed:16207722, PubMed:16251192, PubMed:17327236, PubMed:17371830, PubMed:18835241, PubMed:9094314, PubMed:9368760, PubMed:9445476, PubMed:9707564, PubMed:9768361). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage (PubMed:10226025, PubMed:12167717, PubMed:9094314). Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). Activates PPARG transcriptional activity and promotes adipocyte differentiation (By similarity). Activates the NF-kappa-B pathway via phosphorylation of IKKB (PubMed:16207722). The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II (PubMed:14585963). Controls proliferation, survival, and growth of developing pancreatic cells (By similarity). Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells (By similarity). Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis (PubMed:17371830). Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response (By similarity). Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses (By similarity). Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.
- Post-translational modifications
Phosphorylation on Ser-241 in the activation loop is required for full activity. PDPK1 itself can autophosphorylate Ser-241, leading to its own activation. Autophosphorylation is inhibited by the apoptotic C-terminus cleavage product of PKN2 (By similarity). Tyr-9 phosphorylation is critical for stabilization of both PDPK1 and the PDPK1/SRC complex via HSP90-mediated protection of PDPK1 degradation. Angiotensin II stimulates the tyrosine phosphorylation of PDPK1 in vascular smooth muscle in a calcium- and SRC-dependent manner. Phosphorylated on Tyr-9, Tyr-373 and Tyr-376 by INSR in response to insulin. Palmitate negatively regulates autophosphorylation at Ser-241 and palmitate-induced phosphorylation at Ser-529 and Ser-501 by PKC/PRKCQ negatively regulates its ability to phosphorylate PKB/AKT1. Phosphorylation at Thr-354 by MELK partially inhibits kinase activity, the inhibition is cooperatively enhanced by phosphorylation at Ser-394 and Ser-398 by MAP3K5.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
AKT1 + PKN2 peptide substrate ab204873 (AKT1 + PKN2 peptide substrate) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDPK1 also known as 3-phosphoinositide-dependent protein kinase-1 or PDK1 is a protein kinase with a molecular mass of approximately 63 kDa. It plays an important mechanical role in the activation of other kinases through phosphorylation on threonine or serine residues. PDPK1 is widely expressed in various tissues including heart lung pancreas and liver reflecting its significant roles in cellular signalling.
- Biological function summary
PDPK1 is a master regulator of several AGC kinase family enzymes. It functions as a docking station coordinating with other proteins for activation processes. For example PDPK1 helps in activating PKB/Akt which is required for cell survival and growth. PDPK1 forms part of signaling complexes that manage processes like metabolism growth and survival.
- Pathways
PDPK1 plays an essential role in the PI3K/AKT pathway which is an important signaling pathway for cellular growth and survival. This pathway begins with the activation of PI3K leading to the production of PIP3 which creates a binding site for PDPK1 at the plasma membrane where it activates PKB/Akt. Also PDPK1 interacts with NF-κB pathway components linking it to processes in immune response regulation and inflammation.
- Associated diseases and disorders
PDPK1 has associations with cancer and Type 2 diabetes. Abnormal activity of PDPK1 is observed in several cancer types indicating its role in uncontrolled cell growth. In Type 2 diabetes PDPK1 influences insulin signaling disruptions and glucose metabolism. PDPK1 connects these conditions through interactions with proteins like mTOR which influences cell growth and metabolism further exemplifying its importance in disease mechanisms.
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5 product images
SDS-PAGE - Recombinant human PDPK1 protein (ab60834)
SDS PAGE analysis of ab60834
Functional Studies - Recombinant human PDPK1 protein (ab60834)
Sample Kinase Activity Plot.
SDS-PAGE - Recombinant human PDPK1 protein (ab60834)
ab60834 on SDS-PAGE, MW ~67kDa.
Western blot - Recombinant human PDPK1 protein (ab60834)
All lanes: Anti-PDPK1 antibody (ab31406) at 1/1000 dilution
All lanes: Western blot - Recombinant human PDPK1 protein (ab60834) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10s
Functional Studies - Recombinant human PDPK1 protein (ab60834)
The specific activity of PDPK1 (ab60834) was determined to be 25 nmol /min/mg as per activity assay protocol.
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