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AB101103

Recombinant Human PDXK protein

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Recombinant Human PDXK protein is a Human Full Length protein, in the 1 to 312 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C21orf124, C21orf97, PKH, PNK, PRED79, PDXK, Pyridoxal kinase, Pyridoxine kinase

1 Images
SDS-PAGE - Recombinant Human PDXK protein (AB101103)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PDXK protein (AB101103)

15% SDS-PAGE analysis of ab101103 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

O00764

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMEEECRVLSIQSHVIRGYVGNRAATFPLQVLGFEIDAVNSVQFSNHTGYAHWKGQVLNSDELQELYEGLRLNNMNKYDYVLTGYTRDKSFLAMVVDIVQELKQQNPRLVYVCDPVLGDKWDGEGSMYVPEDLLPVYKEKVVPLADIITPNQFEAELLSGRKIHSQEEALRVMDMLHSMGPDTVVITSSDLPSPQGSNYLIVLGSQRRRNPAGSVVMERIRMDIRKVDAVFVGTGDLFAAMLLAWTHKHPNNLKVACEKTVSTLHHVLQRTIQCAKAQAGEGVRPSPMQLELRMVQSKRDIEDPEIVVQATVL","proteinLength":"Full Length","predictedMolecularWeight":"37.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":312,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00764","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDXK also known as pyridoxal kinase is an important enzyme that facilitates the phosphorylation of vitamin B6 to pyridoxal-5'-phosphate (PLP). Its molecular mass is approximately 35 kDa. You can find it expressed mainly in the liver kidney and brain tissues. PDXK plays a role in converting vitamin B6 which is necessary for various enzymatic activities throughout the body.
Biological function summary

PDXK functions within cellular environments by catalyzing the formation of PLP an active form of vitamin B6. PLP acts as a coenzyme involved in numerous reactions such as amino acid metabolism neurotransmitter synthesis and modulation of hormone activity. PDXK does not work in isolation but instead interacts with other proteins in these processes though it is not a part of a larger complex.

Pathways

PDXK is important for the vitamin B6 metabolism pathway. This pathway integrates with networks involving amino acid catabolism and neurotransmitter pathways essential for metabolic functions and nervous system regulation. An association exists between PDXK and proteins like cysteine desulfurase which also rely on PLP for their enzymatic activities highlighting its involvement in broader biochemical systems.

PDXK has been linked with metabolic disorders and neurological conditions. A deficiency or dysfunction in PDXK activity can contribute to conditions such as pyridoxine-dependent epilepsy and some forms of anemia. In these cases disruptions in the metabolism of vitamin B6 due to inadequate levels of PLP may directly affect proteins like glutamate decarboxylase which require PLP for proper function.
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Specifications

Form

Liquid

Additional notes

ab101103 was purified using conventional chromatography techniques.

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General info

Function

Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable) (PubMed : 10987144, PubMed : 17766369, PubMed : 19351586, PubMed : 31187503, PubMed : 9099727). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.

Sequence similarities

Belongs to the pyridoxine kinase family.

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Product protocols

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Target data

Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable) (PubMed : 10987144, PubMed : 17766369, PubMed : 19351586, PubMed : 31187503, PubMed : 9099727). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.
See full target information PDXK
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Complementary Products

Primary Antibodies

AB251802

Anti-PDXK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDXK antibody (AB251802)

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Cell Lines & Lysates

AB266440

Human PDXK knockout HEK-293T cell line

Sanger Sequencing - Human PDXK knockout HEK-293T cell line (AB266440)

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Cell Lines & Lysates

AB259036

Human PDXK knockout HEK-293T cell lysate

Sanger Sequencing - Human PDXK knockout HEK-293T cell lysate (AB259036)

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