Recombinant Human PDXK.1 protein is a Human Full Length protein, in the 1 to 312 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M E E E C R V L S I Q S H V I R G Y V G N R A A T F P L Q V L G F E I D A V N S V Q F S N H T G Y A H W K G Q V L N S D E L Q E L Y E G L R L N N M N K Y D Y V L T G Y T R D K S F L A M V V D I V Q E L K Q Q N P R L V Y V C D P V L G D K W D G E G S M Y V P E D L L P V Y K E K V V P L A D I I T P N Q F E A E L L S G R K I H S Q E E A L R V M D M L H S M G P D T V V I T S S D L P S P Q G S N Y L I V L G S Q R R R N P A G S V V M E R I R M D I R K V D A V F V G T G D L F A A M L L A W T H K H P N N L K V A C E K T V S T L H H V L Q R T I Q C A K A Q A G E G V R P S P M Q L E L R M V Q S K R D I E D P E I V V Q A T V L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable) (PubMed:10987144, PubMed:17766369, PubMed:19351586, PubMed:31187503, PubMed:9099727). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.
Alternative names
C21orf124, C21orf97, PKH, PNK, PRED79, PDXK, Pyridoxal kinase, Pyridoxine kinase
Recommended products
Recombinant Human PDXK.1 protein is a Human Full Length protein, in the 1 to 312 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O00764-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M E E E C R V L S I Q S H V I R G Y V G N R A A T F P L Q V L G F E I D A V N S V Q F S N H T G Y A H W K G Q V L N S D E L Q E L Y E G L R L N N M N K Y D Y V L T G Y T R D K S F L A M V V D I V Q E L K Q Q N P R L V Y V C D P V L G D K W D G E G S M Y V P E D L L P V Y K E K V V P L A D I I T P N Q F E A E L L S G R K I H S Q E E A L R V M D M L H S M G P D T V V I T S S D L P S P Q G S N Y L I V L G S Q R R R N P A G S V V M E R I R M D I R K V D A V F V G T G D L F A A M L L A W T H K H P N N L K V A C E K T V S T L H H V L Q R T I Q C A K A Q A G E G V R P S P M Q L E L R M V Q S K R D I E D P E I V V Q A T V L
- Accession
- O00764
- Protein length
- Full Length
- Predicted molecular weight
- 37.6 kDa
- Amino acids
- 1 to 312
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab101103 was purified using conventional chromatography techniques.
General info
- Function
Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (Probable) (PubMed:10987144, PubMed:17766369, PubMed:19351586, PubMed:31187503, PubMed:9099727). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions.
- Sequence similarities
Belongs to the pyridoxine kinase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDXK.1 also known as pyridoxal kinase is an important enzyme that facilitates the phosphorylation of vitamin B6 to pyridoxal-5'-phosphate (PLP). Its molecular mass is approximately 35 kDa. You can find it expressed mainly in the liver kidney and brain tissues. PDXK.1 plays a role in converting vitamin B6 which is necessary for various enzymatic activities throughout the body.
- Biological function summary
PDXK.1 functions within cellular environments by catalyzing the formation of PLP an active form of vitamin B6. PLP acts as a coenzyme involved in numerous reactions such as amino acid metabolism neurotransmitter synthesis and modulation of hormone activity. PDXK.1 does not work in isolation but instead interacts with other proteins in these processes though it is not a part of a larger complex.
- Pathways
PDXK.1 is important for the vitamin B6 metabolism pathway. This pathway integrates with networks involving amino acid catabolism and neurotransmitter pathways essential for metabolic functions and nervous system regulation. An association exists between PDXK.1 and proteins like cysteine desulfurase which also rely on PLP for their enzymatic activities highlighting its involvement in broader biochemical systems.
- Associated diseases and disorders
PDXK.1 has been linked with metabolic disorders and neurological conditions. A deficiency or dysfunction in PDXK.1 activity can contribute to conditions such as pyridoxine-dependent epilepsy and some forms of anemia. In these cases disruptions in the metabolism of vitamin B6 due to inadequate levels of PLP may directly affect proteins like glutamate decarboxylase which require PLP for proper function.
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SDS-PAGE - Recombinant Human PDXK.1 protein (ab101103)
15% SDS-PAGE analysis of ab101103 (3μg)
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