Recombinant Human PEA15 protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A E Y G T L L Q D L T N N I T L E D L E Q L K S A C K E D I P S E K S E E I T T G S A W F S F L E S H N K L D K D N L S Y I E H I F E I S R R P D L L T M V V D Y R T R V L K I S E E D E L D T K L T R I P S A K K Y K D I I R Q P S E E E I I K L A P P P K K A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
Alternative names
Astrocytic phosphoprotein PEA-15, 15 kDa phosphoprotein enriched in astrocytes, Phosphoprotein enriched in diabetes, PED, PEA15
Recommended products
Recombinant Human PEA15 protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q15121-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M A E Y G T L L Q D L T N N I T L E D L E Q L K S A C K E D I P S E K S E E I T T G S A W F S F L E S H N K L D K D N L S Y I E H I F E I S R R P D L L T M V V D Y R T R V L K I S E E D E L D T K L T R I P S A K K Y K D I I R Q P S E E E I I K L A P P P K K A
- Accession
- Q15121
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purified by using conventional chromatography.
General info
- Function
Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
- Post-translational modifications
Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PEA15 also known as phosphoprotein enriched in astrocytes 15 or PED is a multifunctional protein weighing around 15 kDa. Mechanically PEA15 acts as a death effector domain (DED)-containing protein that modulates diverse cellular processes by binding to and inhibiting other DED-containing proteins. This protein shows wide expression notably in the central nervous system including the brain where it exerts critical roles in signaling networks.
- Biological function summary
PEA15 plays pivotal roles in promoting cellular survival and regulating apoptosis within various cell types. It operates by modulating pathways like apoptosis and cell proliferation often forming complexes with other DED-containing proteins such as FADD and caspase-8. By interacting with these proteins PEA15 can maintain cellular stability and manage stress responses illustrating its significant contribution to cellular homeostasis.
- Pathways
PEA15 is integral in mediating the extracellular signal-regulated kinase (ERK) and MAP kinase pathways. As PEA15 binds to ERK1/2 it retains these kinases in the cytoplasm impeding their translocation to the nucleus and therefore affecting the transcription of target genes. Furthermore its interactions with Ras-related proteins indicate its involvement in the regulation of cell growth and differentiation showing its broad influence on cellular signaling cascades.
- Associated diseases and disorders
PEA15 correlates with neurological conditions including Alzheimer's disease and gliomas. In Alzheimer's disease PEA15 modulates neuroinflammation and amyloid precursor protein processing collaborating with proteins such as tau and amyloid-beta. In gliomas alterations in PEA15 expression influence tumor progression and treatment susceptibility highlighting important intersections with oncogenic factors like EGFR and PTEN. These associations spotlight PEA15 as a potential therapeutic target in both neurodegenerative and cancerous disorders.
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1 product image
SDS-PAGE - Recombinant Human PEA15 protein (ab87717)
ab87717 on 15% SDS-PAGE (3μg)
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