JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB287924

Recombinant Human PEDF protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human PEDF protein is a Human Fragment protein, in the 20 to 418 aa range, expressed in HEK 293 cells, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC.

View Alternative Names

PEDF, PIG35, SERPINF1, Pigment epithelium-derived factor, Cell proliferation-inducing gene 35 protein, EPC-1, Serpin F1

3 Images
Mass Spectrometry - Recombinant Human PEDF protein (AB287924)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human PEDF protein (AB287924)

Mass determination by ESI TOF : 44444.85 +/- 10Da

SDS-PAGE - Recombinant Human PEDF protein (AB287924)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PEDF protein (AB287924)

SDS-PAGE analysis of ab287924

HPLC - Recombinant Human PEDF protein (AB287924)
  • HPLC

Supplier Data

HPLC - Recombinant Human PEDF protein (AB287924)

HPLC analysis of ab287924

Key facts

Purity

undefined HPLC

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

HPLC, SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P36955

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"QNPASPPEEGSPDPDSTGALVEEEDPFFKVPVNKLAAAVSNFGYDLYRVRSSTSPTTNVLLSPLSVATALSALSLGAEQRTESIIHRALYYDLISSPDIHGTYKELLDTVTAPQKNLKSASRIVFEKKLRIKSSFVAPLEKSYGTRPRVLTGNPRLDLQEINNWVQAQMKGKLARSTKEIPDEISILLLGVAHFKGQWVTKFDSRKTSLEDFYLDEERTVRVPMMSDPKAVLRYGLDSDLSCKIAQLPLTGSMSIIFFLPLKVTQNLTLIEESLTSEFIHDIDRELKTVQAVLTVPKLKLSYEGEVTKSLQEMKLQSLFDSPDFSKITGKPIKLTQVEHRAGFEWNEDGAGTTPSPGLQPAHLTFPLDYHLNQPFIFVLRDTDTGALLFIGKILDPRGP","proteinLength":"Fragment","predictedMolecularWeight":"44.44 kDa","actualMolecularWeight":"44.44 kDa","aminoAcidEnd":418,"aminoAcidStart":20,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P36955","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PEDF also known as pigment epithelium-derived factor is a secreted glycoprotein with a mass of approximately 50 kDa. Its expression is highest in the retina and also occurs in several other tissues including liver and brain. PEDF has neurotrophic and neuroprotective properties. The PEDF protein works mechanically by interacting with receptors on cell surfaces to inhibit angiogenesis and promote neuronal survival and differentiation.
Biological function summary

PEDF plays a significant role in controlling angiogenesis due to its ability to inhibit excessive blood vessel formation. This makes PEDF essential in balancing the vasculature and nutrient availability in tissues. While PEDF does not form part of a larger protein complex its activity influences the biological interplay between cells by stabilizing the extracellular matrix and influencing cell signaling pathways involved in maintaining tissue homeostasis.

Pathways

PEDF interacts directly within the angiogenesis and neuroprotection pathways influencing cellular processes critical for normal tissue function. In angiogenesis PEDF disrupts the VEGF pathway by serving as an inhibitor thereby restricting uncontrolled vessel growth. It also plays a role in neuroprotection pathways where it cooperates with proteins like nerve growth factor to enhance neuronal survival and repair processes.

PEDF relates significantly to diabetic retinopathy and cancer. PEDF's angiogenesis inhibition has therapeutic implications in diabetic retinopathy where unregulated blood vessel growth leads to vision loss. It also shows potential in cancer therapy by targeting tumor angiogenesis. In both conditions its interaction with VEGF is notable as it competes to modulate angiogenesis with potential consequences for disease progression and treatment strategies.
style
left-column

Specifications

Form

Lyophilized

Additional notes

>= 95 % HPLC

style
left-column

General info

Function

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Sequence similarities

Belongs to the serpin family.

Post-translational modifications

The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.. N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

style
left-column

Product protocols

style
left-column

Target data

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
See full target information SERPINF1
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB246535

Human PEDF ELISA Kit

Recombinant|SimpleStep

ELISA - Human PEDF ELISA Kit (AB246535)

  • 0
  • 0
Primary Antibodies

AB307083

Anti-PEDF antibody [EPR25141-150]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PEDF antibody [EPR25141-150] (AB307083)

  • 0
  • 1
Primary Antibodies

AB67225

Anti-Plxdc2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Plxdc2 antibody (AB67225)

  • 0
  • 0
Proteins & Peptides

AB132669

Recombinant Human CYP11A1 protein

SDS-PAGE - Recombinant Human CYP11A1 protein (AB132669)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com