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Recombinant Human PEDF protein is a Human Fragment protein, in the 20 to 418 aa range, expressed in HEK 293, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.

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Key facts

Purity

HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, MS, HPLC

Biologically active

No

Amino acid sequence

Q N P A S P P E E G S P D P D S T G A L V E E E D P F F K V P V N K L A A A V S N F G Y D L Y R V R S S T S P T T N V L L S P L S V A T A L S A L S L G A E Q R T E S I I H R A L Y Y D L I S S P D I H G T Y K E L L D T V T A P Q K N L K S A S R I V F E K K L R I K S S F V A P L E K S Y G T R P R V L T G N P R L D L Q E I N N W V Q A Q M K G K L A R S T K E I P D E I S I L L L G V A H F K G Q W V T K F D S R K T S L E D F Y L D E E R T V R V P M M S D P K A V L R Y G L D S D L S C K I A Q L P L T G S M S I I F F L P L K V T Q N L T L I E E S L T S E F I H D I D R E L K T V Q A V L T V P K L K L S Y E G E V T K S L Q E M K L Q S L F D S P D F S K I T G K P I K L T Q V E H R A G F E W N E D G A G T T P S P G L Q P A H L T F P L D Y H L N Q P F I F V L R D T D T G A L L F I G K I L D P R G P

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Application

HPLC

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Alternative names

Recommended products

Recombinant Human PEDF protein is a Human Fragment protein, in the 20 to 418 aa range, expressed in HEK 293, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, MS, HPLC.

Key facts

Purity

HPLC

Endotoxin level

<= 0.005 EU/µg

Expression system

HEK 293 cells

Applications

SDS-PAGE, MS, HPLC

Accession
P36955-1
Animal free

Yes

Species

Human

Reconstitution

Reconstitute in PBS

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

Sequence info

Amino acid sequence

Q N P A S P P E E G S P D P D S T G A L V E E E D P F F K V P V N K L A A A V S N F G Y D L Y R V R S S T S P T T N V L L S P L S V A T A L S A L S L G A E Q R T E S I I H R A L Y Y D L I S S P D I H G T Y K E L L D T V T A P Q K N L K S A S R I V F E K K L R I K S S F V A P L E K S Y G T R P R V L T G N P R L D L Q E I N N W V Q A Q M K G K L A R S T K E I P D E I S I L L L G V A H F K G Q W V T K F D S R K T S L E D F Y L D E E R T V R V P M M S D P K A V L R Y G L D S D L S C K I A Q L P L T G S M S I I F F L P L K V T Q N L T L I E E S L T S E F I H D I D R E L K T V Q A V L T V P K L K L S Y E G E V T K S L Q E M K L Q S L F D S P D F S K I T G K P I K L T Q V E H R A G F E W N E D G A G T T P S P G L Q P A H L T F P L D Y H L N Q P F I F V L R D T D T G A L L F I G K I L D P R G P

Accession

P36955

Protein length

Fragment

Predicted molecular weight

44.44 kDa

Amino acids

20 to 418

Nature

Recombinant

Specifications

Form

Lyophilized

Additional notes

>= 95 % HPLC

General info

Function

Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Sequence similarities

Belongs to the serpin family.

Post-translational modifications

The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PEDF also known as pigment epithelium-derived factor is a secreted glycoprotein with a mass of approximately 50 kDa. Its expression is highest in the retina and also occurs in several other tissues including liver and brain. PEDF has neurotrophic and neuroprotective properties. The PEDF protein works mechanically by interacting with receptors on cell surfaces to inhibit angiogenesis and promote neuronal survival and differentiation.

Biological function summary

PEDF plays a significant role in controlling angiogenesis due to its ability to inhibit excessive blood vessel formation. This makes PEDF essential in balancing the vasculature and nutrient availability in tissues. While PEDF does not form part of a larger protein complex its activity influences the biological interplay between cells by stabilizing the extracellular matrix and influencing cell signaling pathways involved in maintaining tissue homeostasis.

Pathways

PEDF interacts directly within the angiogenesis and neuroprotection pathways influencing cellular processes critical for normal tissue function. In angiogenesis PEDF disrupts the VEGF pathway by serving as an inhibitor thereby restricting uncontrolled vessel growth. It also plays a role in neuroprotection pathways where it cooperates with proteins like nerve growth factor to enhance neuronal survival and repair processes.

Associated diseases and disorders

PEDF relates significantly to diabetic retinopathy and cancer. PEDF's angiogenesis inhibition has therapeutic implications in diabetic retinopathy where unregulated blood vessel growth leads to vision loss. It also shows potential in cancer therapy by targeting tumor angiogenesis. In both conditions its interaction with VEGF is notable as it competes to modulate angiogenesis with potential consequences for disease progression and treatment strategies.

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