Recombinant Human Pellino 1 protein is a Human Fragment protein, in the 145 to 404 aa range, expressed in Escherichia coli, with =80% purity and suitable for SDS-PAGE.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed:12496252, PubMed:17675297, PubMed:29883609, PubMed:30952868). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6 (PubMed:12496252, PubMed:17675297). Acts as a positive regulator of inflammatory response in microglia through activation of NF-kappa-B and MAP kinase (By similarity). Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:12496252, PubMed:17675297). Conjugates 'Lys-63'-linked ubiquitin chains to the adapter protein ASC/PYCARD, which in turn is crucial for NLRP3 inflammasome activation (PubMed:34706239). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (PubMed:29883609). Following phosphorylation by ATM, catalyzes 'Lys-63'-linked ubiquitination of NBN, promoting DNA repair via homologous recombination (PubMed:30952868). Negatively regulates activation of the metabolic mTORC1 signaling pathway by mediating 'Lys-63'-linked ubiquitination of mTORC1-inhibitory protein TSC1 and thereby promoting TSC1/TSC2 complex stability (PubMed:33215753).
PRISM, PELI1, E3 ubiquitin-protein ligase pellino homolog 1, Pellino-1, Pellino-related intracellular-signaling molecule, RING-type E3 ubiquitin transferase pellino homolog 1
Recombinant Human Pellino 1 protein is a Human Fragment protein, in the 145 to 404 aa range, expressed in Escherichia coli, with =80% purity and suitable for SDS-PAGE.
Constituents: 0.58% Sodium chloride, 0.32% Tris HCl
Purified via His tag
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed:12496252, PubMed:17675297, PubMed:29883609, PubMed:30952868). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6 (PubMed:12496252, PubMed:17675297). Acts as a positive regulator of inflammatory response in microglia through activation of NF-kappa-B and MAP kinase (By similarity). Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation (PubMed:12496252, PubMed:17675297). Conjugates 'Lys-63'-linked ubiquitin chains to the adapter protein ASC/PYCARD, which in turn is crucial for NLRP3 inflammasome activation (PubMed:34706239). Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3 (PubMed:29883609). Negatively regulates necroptosis by reducing RIPK3 expression (PubMed:29883609). Mediates 'Lys-63'-linked ubiquitination of RIPK1 (PubMed:29883609). Following phosphorylation by ATM, catalyzes 'Lys-63'-linked ubiquitination of NBN, promoting DNA repair via homologous recombination (PubMed:30952868). Negatively regulates activation of the metabolic mTORC1 signaling pathway by mediating 'Lys-63'-linked ubiquitination of mTORC1-inhibitory protein TSC1 and thereby promoting TSC1/TSC2 complex stability (PubMed:33215753).
Belongs to the pellino family.
Phosphorylation by IRAK1 and IRAK4 enhances its E3 ligase activity (PubMed:17997719). Phosphorylated by ATM in response to DNA damage, promoting localization to DNA double-strand breaks (DSBs) and ability to mediate 'Lys-63'-linked ubiquitination of NBN (PubMed:30952868).
Pellino 1 is an E3 ubiquitin ligase important in signaling pathways linked to immune response and inflammation. It is also known as RNF232 and has a molecular weight of approximately 41 kDa. You can find expression of Pellino 1 in various tissues including immune cells like macrophages and dendritic cells. Its activity relies on its ability to add ubiquitin moieties to target proteins a process essential for tagging these proteins for cellular processes like degradation or trafficking.
Pellino 1 plays a major role in immune signaling networks. It acts as an adaptor and signal transducer which facilitates the recruitment and regulation of other signaling molecules. It often forms part of a signaling complex within the Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) pathways. The complex assembly enables Pellino 1 to modulate innate immune responses by influencing downstream signaling events that elevate inflammatory cytokine production and immune cell activation.
Pellino 1 participates significantly in the TLR and IL-1R signaling pathways. These pathways are critical for activating nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways enabling transcription of immune-related genes. In the TLR pathway Pellino 1 functions alongside proteins such as IRAK1 modulating pathway outcomes by regulating the ubiquitination of downstream signaling components.
Pellino 1 frequently associates with autoimmune diseases and chronic inflammatory conditions. Its role in modulating immune responses links it to conditions such as rheumatoid arthritis where it influences the inflammatory response through interactions with key proteins like TRAF6. Aberrations in Pellino 1 function or expression have also connections to systemic lupus erythematosus as its regulation of NF-κB signaling affects autoantibody production and inflammatory cell survival.
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