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AB132822

Recombinant Human Pellino-3 protein

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Recombinant Human Pellino-3 protein is a Human Full Length protein, in the 1 to 331 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

E3 ubiquitin-protein ligase pellino homolog 3, Pellino-3, PELI3

1 Images
SDS-PAGE - Recombinant Human Pellino-3 protein (AB132822)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Pellino-3 protein (AB132822)

12.5% SDS-PAGE analysis of ab132822 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q8N2H9

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PELI3.
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Sequence info

[{"sequence":"MVLEGNPEVGSPRTSDLQHRGNKGSCVLSSPGEDAQPGEEPIKYGELIVLGCCEEGGEETEAQRGEVTGPRAHSCYNGCLASGDKGRRRSRLALSRRSHANGVKPDVMHHISTPLVSKALSNRGQHSISYTLSRSHSVIVEYTHDSDTDMFQIGRSTENMIDFVVTDTSPGGGAAEGPSAQSTISRYACRILCDRRPPYTARIYAAGFDASSNIFLGERAAKWRTPDGLMDGLTTNGVLVMHPAGGFSEDSAPGVWREISVCGNVYTLRDSRSAQQRGKLVGGPLHSPPLSLPGPHKLPIPLPKPGDDLQSFCPREPQQAPYQGIPGPGGS","proteinLength":"Full Length","predictedMolecularWeight":"61.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":331,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8N2H9","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pellino-3 also called PEK3 functions as an important E3 ubiquitin ligase in multiple cellular processes. This protein with an approximate mass of 50 kDa promotes the ubiquitination of target proteins which can signal for their degradation or alter their activity. Pellino-3 displays expression in a variety of human tissues including immune cells where it supports immune responses. Researchers have extensively studied its role in intracellular signaling networks due to its ability to modify other proteins through ubiquitination.
Biological function summary

The Pellino-3 protein participates in the regulation of immune signaling pathways and is frequently associated with the Toll/Interleukin-1 receptor (TIR) superfamily complex. It plays an important role in modulating the activity of TIRAP/Mal and MyD88 two adaptors that are important for signaling through these pathways. By modifying signaling proteins in immune cells Pellino-3 helps control the overall immune response and inflammation levels within the body.

Pathways

Pellino-3 integrates into critical immune regulatory pathways particularly the NF-κB and MAPK pathways. In the NF-κB pathway Pellino-3 ubiquitinates intermediates like TRAF6 which subsequently triggers downstream signaling leading to inflammation and immune responses. Interaction with proteins in the MAPK pathway shows how Pellino-3 can influence the cellular response to stress or pathogens. Robust regulation of these pathways maintains homeostasis and protects against excessive immune reactions.

Pellino-3 influences the development and progression of autoimmune diseases and inflammatory disorders. Elevated activity or expression of Pellino-3 links to conditions such as rheumatoid arthritis where dysregulated immune signaling occurs. The protein also interacts with other proteins like MyD88 in these disorders highlighting its potential as a therapeutic target. Understanding the role of Pellino-3 in these contexts may offer new strategies for managing immune-related diseases.
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Specifications

Form

Liquid

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General info

Function

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed : 12874243, PubMed : 17675297). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6 (PubMed : 12874243). Mediates 'Lys-63'-linked polyubiquitination of IRAK1 (PubMed : 12874243). Can activate AP1/JUN and ELK1 (PubMed : 12874243). Acts as a regulator of innate immunity by mediating 'Lys-63'-linked polyubiquitination of RIPK2 downstream of NOD1 and NOD2, thereby transforming RIPK2 into a scaffolding protein for downstream effectors, ultimately leading to activation of the NF-kappa-B and MAP kinases signaling (By similarity). Catalyzes 'Lys-63'-linked polyubiquitination of RIPK2 in parallel of XIAP (By similarity).

Sequence similarities

Belongs to the pellino family.

Post-translational modifications

Phosphorylated by IRAK1 enhancing its E3 ligase activity.

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Product protocols

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Target data

E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed : 12874243, PubMed : 17675297). Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6 (PubMed : 12874243). Mediates 'Lys-63'-linked polyubiquitination of IRAK1 (PubMed : 12874243). Can activate AP1/JUN and ELK1 (PubMed : 12874243). Acts as a regulator of innate immunity by mediating 'Lys-63'-linked polyubiquitination of RIPK2 downstream of NOD1 and NOD2, thereby transforming RIPK2 into a scaffolding protein for downstream effectors, ultimately leading to activation of the NF-kappa-B and MAP kinases signaling (By similarity). Catalyzes 'Lys-63'-linked polyubiquitination of RIPK2 in parallel of XIAP (By similarity).
See full target information PELI3
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