Recombinant Human Peregrin/BRPF1 protein is a Human Fragment protein, in the 628 to 746 aa range, expressed in Escherichia coli, with >=69% purity and suitable for SDS-PAGE.
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Publications
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- ChemMedChem 19:e2023005902024Accelerating BRPF1b hit identification with BioPhysical and Active Learning Screening (BioPALS).Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandeep Pal,Zandile Nare,Vincenzo A Rao,Brian O Smith,Ian Morrison,Edward A Fitzgerald,Andrew Scott,Matilda J Bingham,Thomas PesnotPubMed 38372199
Key facts
- Purity
- >=69% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M H H H H H H M Q L T P F L I L L R K T L E Q L Q E K D T G N I F S E P V P L S E V T E L D E V P D Y L D H I K K P M D F F T M K Q N L E A Y R Y L N F D D F E E D F N L I V S N C L K Y N A K D T I F Y R A A V R L R E Q G G A V L R Q A R R Q A E K M G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PubMed:16387653, PubMed:24065767, PubMed:27939640). Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PubMed:24065767). Some HAT complexes preferentially mediate histone H3 'Lys-23' (H3K23ac) acetylation (PubMed:27939640). Positively regulates the transcription of RUNX1 and RUNX2 (PubMed:18794358).
Alternative names
BR140, BRPF1, Peregrin, Bromodomain and PHD finger-containing protein 1, Protein Br140
Recommended products
Recombinant Human Peregrin/BRPF1 protein is a Human Fragment protein, in the 628 to 746 aa range, expressed in Escherichia coli, with >=69% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=69% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P55201-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M H H H H H H M Q L T P F L I L L R K T L E Q L Q E K D T G N I F S E P V P L S E V T E L D E V P D Y L D H I K K P M D F F T M K Q N L E A Y R Y L N F D D F E E D F N L I V S N C L K Y N A K D T I F Y R A A V R L R E Q G G A V L R Q A R R Q A E K M G
- Accession
- P55201
- Protein length
- Fragment
- Predicted molecular weight
- 15.1 kDa
- Amino acids
- 628 to 746
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PubMed:16387653, PubMed:24065767, PubMed:27939640). Plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PubMed:24065767). Some HAT complexes preferentially mediate histone H3 'Lys-23' (H3K23ac) acetylation (PubMed:27939640). Positively regulates the transcription of RUNX1 and RUNX2 (PubMed:18794358).
- Post-translational modifications
Acetylated by KAT6A.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Peregrin
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Peregrin/BRPF1 also known as Peregrins is a protein with a molecular mass around 130 kDa. It functions primarily as a component of the MOZ/MORF histone acetyltransferase (HAT) complex. Peregrin/BRPF1 plays a mechanical role in the regulation of gene expression by altering chromatin structure through histone modification. It is expressed in various tissues with higher expression in the brain and testis indicating its importance in neurological and reproductive functions.
- Biological function summary
Peregrin/BRPF1 is a part of the MOZ/MORF complex which is important for acetylating histone H3 at lysine 23 influencing transcriptional activation. This action aids in transcription regulation and chromatin remodeling facilitating access of transcription factors to DNA. By participating in these processes Peregrin/BRPF1 helps maintain proper gene expression patterns essential for normal cell function and development.
- Pathways
BRPF1 has roles in processes like the cell cycle and embryonic development pathways because its histone acetylation influences gene expression. It interacts with other acetyltransferases such as EP300 and CREBBP which play key roles in transcription regulation and chromatin remodeling within these pathways. These interactions highlight BRPF1's critical involvement in coordinating cellular events and developmental processes.
- Associated diseases and disorders
Dysregulation of BRPF1 is associated with neurological disorders such as intellectual disability and specific cancers due to its role in chromatin remodeling and gene expression. In the context of intellectual disability it interacts with other proteins involved in chromatin structure like NSD1 and KDM6A. These connections illustrate the significant impact of Peregrin/BRPF1's regulation and its influence on health when its function becomes impaired.
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1 product image
SDS-PAGE - Recombinant Human Peregrin/BRPF1 protein (ab196416)
4-20% SDS-PAGE analysis of 3 μg ab196416 with Coomassie staining.
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