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AB181932

Recombinant Human Perilipin-2 protein (denatured) (T7 tag N-Terminus)

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(2 Publications)

Recombinant Human Perilipin-2 protein (denatured) (T7 tag N-Terminus) is a Human Full Length protein, in the 1 to 437 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

ADFP, PLIN2, Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP

1 Images
SDS-PAGE - Recombinant Human Perilipin-2 protein (denatured) (T7 tag N-Terminus) (AB181932)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Perilipin-2 protein (denatured) (T7 tag N-Terminus) (AB181932)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

T7 tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q99541

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASMTGGQQMGRGSMASVAVDPQPSVVTRVVNLPLVSSTYDLMSSAYLSTKDQYPYLKSVCEMAENGVKTITSVAMTSALPIIQKLEPQIAVANTYACKGLDRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH","proteinLength":"Full Length","predictedMolecularWeight":"49.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":437,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q99541","tags":[{"tag":"T7","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Perilipin-2 also known as PLIN2 ADFP or adipophilin has an approximate molecular weight of 48 kDa. It is primarily expressed in tissues involved in lipid metabolism like adipose tissue liver and muscle. The main role of Perilipin-2 is to coat lipid droplets in cells thereby regulating lipid storage and breakdown. This protein serves as a critical component of the lipid droplet surface providing structure and protecting the lipid core from excessive unregulated lipolysis.
Biological function summary

Perilipin-2 is a member of the PAT family proteins involved in lipid droplet formation and maintenance. It does not typically form part of larger complexes but it plays a significant role in cellular energy regulation by controlling lipid reserves. This regulation ensures an efficient balance between energy storage and mobilization. The presence and levels of Perilipin-2 can influence how cells respond to nutrients particularly during periods of energy surplus or fasting.

Pathways

Perilipin-2 is heavily involved in the regulation of lipid metabolic pathways and the storage-related signaling cascades. It interacts with key metabolic proteins including hormone-sensitive lipase (HSL) to modulate lipolysis. By doing so Perilipin-2 influences processes like fatty acid mobilization and glycerol release integrating perfectly into the cellular energy balance system. The protein is also related to perilipin another member of the lipid droplet-associated proteins emphasizing its participation in lipid metabolism.

Altered expression of Perilipin-2 can have implications in obesity and fatty liver disease. The protein is involved in the accumulation of lipid droplets which is a common feature in these conditions. Obesity and associated disorders often show elevated levels of Perilipin-2 which mirrors increased lipid storage capacity. The protein’s interplay with adipose tissue composition highlights its connection to obesity-related factors including other lipid droplet-associated proteins like adipose triglyceride lipase (ATGL) which influence the progression of metabolic diseases.
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Specifications

Form

Liquid

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General info

Function

Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.

Sequence similarities

Belongs to the perilipin family.

Post-translational modifications

Acylated; primarily with C14, C16 and C18 fatty acids.. Phosphorylation at Tyr-232 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis.

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Product protocols

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Target data

Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
See full target information PLIN2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36768395

2023

Bilayer-Embedded Lipid Droplets Coated with Perilipin-2 Display a Pancake Shape.

Applications

Unspecified application

Species

Unspecified reactive species

Sevde Puza,Shima Asfia,Ralf Seemann,Jean-Baptiste Fleury

Biochimica et biophysica acta. Biomembranes 1865:184074 PubMed36283490

2022

Phospholipids diffusion on the surface of model lipid droplets.

Applications

Unspecified application

Species

Unspecified reactive species

Shima Asfia,Ralf Seemann,Jean-Baptiste Fleury
View all publications
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Complementary Products

Primary Antibodies

AB108323

Anti-Perilipin 2 antibody [EPR3713]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-Perilipin 2 antibody [EPR3713] (AB108323)

  • 4
  • 3
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Proteins & Peptides

AB186077

Recombinant Human TRIB3 protein

SDS-PAGE - Recombinant Human TRIB3 protein (AB186077)

  • 0
  • 0
Primary Antibodies

AB3526

Anti-Perilipin-1 antibody

Immunocytochemistry - Anti-Perilipin-1 antibody (AB3526)

  • 4
  • 16

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