Recombinant Human Periostin protein (His Tag) is a Human Fragment protein, in the 22 to 781 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
Amino acid sequence
N N H Y D K I L A H S R I R G R D Q G P N V C A L Q Q I L G T K K K Y F S T C K N W Y K K S I C G Q K T T V L Y E C C P G Y M R M E G M K G C P A V L P I D H V Y G T L G I V G A T T T Q R Y S D A S K L R E E I E G K G S F T Y F A P S N E A W D N L D S D I R R G L E S N V N V E L L N A L H S H M I N K R M L T K D L K N G M I I P S M Y N N L G L F I N H Y P N G V V T V N C A R I I H G N Q I A T N G V V H V I D R V L T Q I G T S I Q D F I E A E D D L S S F R A A A I T S D I L E A L G R D G H F T L F A P T N E A F E K L P R G V L E R I M G D K V A S E A L M K Y H I L N T L Q C S E S I M G G A V F E T L E G N T I E I G C D G D S I T V N G I K M V N K K D I V T N N G V I H L I D Q V L I P D S A K Q V I E L A G K Q Q T T F T D L V A Q L G L A S A L R P D G E Y T L L A P V N N A F S D D T L S M D Q R L L K L I L Q N H I L K V K V G L N E L Y N G Q I L E T I G G K Q L R V F V Y R T A V C I E N S C M E K G S K Q G R N G A I H I F R E I I K P A E K S L H E K L K Q D K R F S T F L S L L E A A D L K E L L T Q P G D W T L F V P T N D A F K G M T S E E K E I L I R D K N A L Q N I I L Y H L T P G V F I G K G F E P G V T N I L K T T Q G S K I F L K E V N D T L L V N E L K S K E S D I M T T N G V I H V V D K L L Y P A D T P V G N D Q L L E I L N K L I K Y I Q I K F V R G S T F K E I P V T V Y R P T L T K V K I E G E P E F R L I K E G E T I T E V I H G E P I I K K Y T K I I D G V P V E I T E K E T R E E R I I T G P E I K Y T R I S T G G G E T E E T L K K L L Q E D T P V R K L Q A N K K V Q G S R R R L R E G R S Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
Alternative names
OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2
Recommended products
Recombinant Human Periostin protein (His Tag) is a Human Fragment protein, in the 22 to 781 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for HPLC, MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- HPLC, MS, SDS-PAGE
- Biologically active
- No
- Accession
- Q15063-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- N N H Y D K I L A H S R I R G R D Q G P N V C A L Q Q I L G T K K K Y F S T C K N W Y K K S I C G Q K T T V L Y E C C P G Y M R M E G M K G C P A V L P I D H V Y G T L G I V G A T T T Q R Y S D A S K L R E E I E G K G S F T Y F A P S N E A W D N L D S D I R R G L E S N V N V E L L N A L H S H M I N K R M L T K D L K N G M I I P S M Y N N L G L F I N H Y P N G V V T V N C A R I I H G N Q I A T N G V V H V I D R V L T Q I G T S I Q D F I E A E D D L S S F R A A A I T S D I L E A L G R D G H F T L F A P T N E A F E K L P R G V L E R I M G D K V A S E A L M K Y H I L N T L Q C S E S I M G G A V F E T L E G N T I E I G C D G D S I T V N G I K M V N K K D I V T N N G V I H L I D Q V L I P D S A K Q V I E L A G K Q Q T T F T D L V A Q L G L A S A L R P D G E Y T L L A P V N N A F S D D T L S M D Q R L L K L I L Q N H I L K V K V G L N E L Y N G Q I L E T I G G K Q L R V F V Y R T A V C I E N S C M E K G S K Q G R N G A I H I F R E I I K P A E K S L H E K L K Q D K R F S T F L S L L E A A D L K E L L T Q P G D W T L F V P T N D A F K G M T S E E K E I L I R D K N A L Q N I I L Y H L T P G V F I G K G F E P G V T N I L K T T Q G S K I F L K E V N D T L L V N E L K S K E S D I M T T N G V I H V V D K L L Y P A D T P V G N D Q L L E I L N K L I K Y I Q I K F V R G S T F K E I P V T V Y R P T L T K V K I E G E P E F R L I K E G E T I T E V I H G E P I I K K Y T K I I D G V P V E I T E K E T R E E R I I T G P E I K Y T R I S T G G G E T E E T L K K L L Q E D T P V R K L Q A N K K V Q G S R R R L R E G R S Q
- Accession
- Q15063
- Protein length
- Fragment
- Predicted molecular weight
- 86.4 kDa
- Amino acids
- 22 to 781
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Specifications
- Form
- Lyophilized
- Additional notes
SDS-PAGE >= 95%
General info
- Function
Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
- Post-translational modifications
Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.
- Biological function summary
The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.
- Pathways
Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.
- Associated diseases and disorders
The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
HPLC - Recombinant Human Periostin protein (His Tag) (ab310788)
HPLC analysis of ab310788
SDS-PAGE - Recombinant Human Periostin protein (His Tag) (ab310788)
SDS-PAGE analysis of ab310788
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com