Recombinant Human Peroxiredoxin 1/PAG protein (His tag) is a Human Full Length protein, in the 1 to 199 aa range, expressed in Yeast, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M S S G N A K I G H P A P N F K A T A V M P D G Q F K D I S L S D Y K G K Y V V F F F Y P L D F T F V C P T E I I A F S D R A E E F K K L N C Q V I G A S V D S H F C H L A W V N T P K K Q G G L G P M N I P L V S D P K R T I A Q D Y G V L K A D E G I S F R G L F I I D D K G I L R Q I T V N D L P V G R S V D E T L R L V Q A F Q F T D K H G E V C P A G W K P G S D T I K P D V Q K S K E Y F S K Q K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (PubMed:9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).
Alternative names
PAGA, PAGB, TDPX2, PRDX1, Peroxiredoxin-1, Natural killer cell-enhancing factor A, Proliferation-associated gene protein, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, NKEF-A, PAG
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Recombinant Human Peroxiredoxin 1/PAG protein (His tag) is a Human Full Length protein, in the 1 to 199 aa range, expressed in Yeast, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Yeast
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q06830-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- M S S G N A K I G H P A P N F K A T A V M P D G Q F K D I S L S D Y K G K Y V V F F F Y P L D F T F V C P T E I I A F S D R A E E F K K L N C Q V I G A S V D S H F C H L A W V N T P K K Q G G L G P M N I P L V S D P K R T I A Q D Y G V L K A D E G I S F R G L F I I D D K G I L R Q I T V N D L P V G R S V D E T L R L V Q A F Q F T D K H G E V C P A G W K P G S D T I K P D V Q K S K E Y F S K Q K
- Accession
- Q06830
- Protein length
- Full Length
- Predicted molecular weight
- 24.6 kDa
- Amino acids
- 1 to 199
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (PubMed:9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).
- Sequence similarities
Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
- Post-translational modifications
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Peroxiredoxin 1 also known as PAG plays an important role as an antioxidant enzyme. It is part of the peroxiredoxin family with a molecular mass of around 22 kDa. This protein helps reduce hydrogen peroxide and organic hydroperoxides to water and corresponding alcohols using reducing equivalents provided by thiol-containing donor molecules. Peroxiredoxin 1/PAG expresses widely in human tissues and cells including liver and erythrocytes reflecting its involvement in cellular redox regulation.
- Biological function summary
Peroxiredoxin 1 helps maintain cellular homeostasis by protecting cells from oxidative stress. It forms homodimers or interacts with other proteins to propagate cellular responses to changes in redox state. As an important member of the antioxidant defense mechanism it contributes to cellular signaling through modulation of hydrogen peroxide signaling pathways. The protein also plays a role in cell proliferation and apoptosis making it significant for cellular health and disease prevention.
- Pathways
Peroxiredoxin 1 participates in critical processes such as the MAPK signaling and TGF-beta pathways. It interacts with MAPK-related proteins influencing cell survival and growth. Peroxiredoxin 1 indirectly modulates downstream signaling molecules that contribute to cellular responses. In these pathways the protein's antioxidant properties allow regulation of reactive oxygen species levels which directly affect cellular signaling cascades.
- Associated diseases and disorders
Peroxiredoxin 1 is relevant to cancer and neurodegenerative disorders. Overexpression or misregulation of this protein has been linked to various cancers where it potentially interacts with oncogenic proteins like c-Myc to promote tumor progression. Additionally abnormal levels of Peroxiredoxin 1 associate with neurodegenerative diseases as oxidative stress is a common factor in their pathology. These connections suggest the protein as a promising target in therapeutic interventions for these diseases.
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1 product image
SDS-PAGE - Recombinant Human Peroxiredoxin 1/PAG protein (His tag) (ab241454)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab241454.
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