Recombinant Human Peroxiredoxin 1/PAG Protein Standard
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Recombinant Human Peroxiredoxin 1/PAG Protein Standard ab327749 is a Human Fragment protein expressed in Escherichia coli suitable for SDS-PAGE, sELISA..<br><br>- Domain: Thioredoxin<br>- Localization: Intracellular<br>- Proximate region: C-terminus<br>- Tag free<br>- >80% SDS-PAGE
View Alternative Names
PAGA, PAGB, TDPX2, PRDX1, Peroxiredoxin-1, Natural killer cell-enhancing factor A, Proliferation-associated gene protein, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2, Thioredoxin-dependent peroxiredoxin 1, NKEF-A, PAG
- SDS-PAGE
Lab
SDS-PAGE - Recombinant Human Peroxiredoxin 1/PAG Protein Standard (AB327749)
SDS-PAGE analysis of ab327749 under reducing conditions for 2ug protein.
Reactivity data
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.
Sequence info
Properties and storage information
Form
Purification technique
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
General info
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (PubMed : 9497357). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).
Sequence similarities
Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
Post-translational modifications
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.. Acetylation increases reducing activity and resistance to superoxidation (PubMed:18606987). Deacetylated by HDAC6 which decreases reducing activity (PubMed:18606987).. The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.
Target data
Product promise
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