Recombinant human Peroxiredoxin 3/PRDX3 protein is a Human Full Length protein, in the 63 to 256 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M P A V T Q H A P Y F K G T A V V N G E F K D L S L D D F K G K Y L V L F F Y P L D F T F V C P T E I V A F S D K A N E F H D V N C E V V A V S V D S H F S H L A W I N T P R K N G G L G H M N I A L L S D L T K Q I S R D Y G V L L E G S G L A L R G L F I I D P N G V I K H L S V N D L P V G R S V E E T L R L V K A F Q Y V E T H G E V C P A N W T P D S P T I K P S P A A S K E Y F Q K V N Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Specific activity: approximately 82-83 pmole/min/µg. |
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Target data
Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:17707404, PubMed:29438714, PubMed:33889951, PubMed:7733872). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477). Required for the maintenance of physical strength (By similarity).
Alternative names
AOP1, PRDX3, Antioxidant protein 1, HBC189, Peroxiredoxin III, Peroxiredoxin-3, Protein MER5 homolog, Thioredoxin-dependent peroxiredoxin 3, AOP-1, Prx-III
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Recombinant human Peroxiredoxin 3/PRDX3 protein is a Human Full Length protein, in the 63 to 256 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity: approximately 82-83 pmole/min/μg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX3 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute Specific activity: approximately 82-83 pmole/min/μg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX3 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute
- Accession
- P30048-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M P A V T Q H A P Y F K G T A V V N G E F K D L S L D D F K G K Y L V L F F Y P L D F T F V C P T E I V A F S D K A N E F H D V N C E V V A V S V D S H F S H L A W I N T P R K N G G L G H M N I A L L S D L T K Q I S R D Y G V L L E G S G L A L R G L F I I D P N G V I K H L S V N D L P V G R S V E E T L R L V K A F Q Y V E T H G E V C P A N W T P D S P T I K P S P A A S K E Y F Q K V N Q
- Accession
- P30048
- Protein length
- Full Length
- Amino acids
- 63 to 256
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab85559 was purified by using conventional chromatography techniques
General info
- Function
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (PubMed:17707404, PubMed:29438714, PubMed:33889951, PubMed:7733872). Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol (PubMed:12492477). Required for the maintenance of physical strength (By similarity).
- Sequence similarities
Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.
- Post-translational modifications
Phosphorylated by LRRK2; phosphorylation reduces perodixase activity.
- Subcellular localisation
- Mitochondrion, Early endosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product was previously labelled as Peroxiredoxin 3
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Peroxiredoxin 3 also known as PRDX3 is an antioxidant enzyme that plays a critical role in the reduction of hydrogen peroxide to water. This protein has a mass of around 25 kDa and belongs to the thioredoxin peroxidase family. It functions within the mitochondria and is expressed in a wide variety of tissues with higher levels in the heart liver and kidney. PRDX3 acts as a protective buffer against oxidative stress by maintaining redox balance in cells.
- Biological function summary
The enzyme helps to safeguard cells from oxidative damage by reducing peroxides produced during cellular metabolism. PRDX3 participates in antioxidant defense mechanisms and may form functional complexes with other proteins to enhance its peroxidase activity. Its antioxidant properties contribute to the maintenance of mitochondrial integrity and influence cellular survival and apoptosis processes.
- Pathways
PRDX3 integrates into the cellular antioxidant defense system prominently in the thioredoxin pathway. In this pathway PRDX3 synergizes with thioredoxin to neutralize harmful oxidative molecules. PRDX3's activity is interconnected with proteins such as thioredoxin and glutaredoxin which also partake in counteracting oxidative stress bolstering an organism's defense against cellular damage.
- Associated diseases and disorders
Alterations in PRDX3 activity associate with conditions like cancer and neurodegenerative disorders. Overexpression of PRDX3 has been observed in certain types of cancer suggesting a potential role in tumor growth through its influence on cell proliferation and survival. In neurodegenerative diseases dysfunctional PRDX3 contributes to neuronal injury. It interacts with proteins like DJ-1 which relate to pathways involved in Parkinson's disease indicating its impact on maintaining neuronal health.
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SDS-PAGE - Recombinant human Peroxiredoxin 3/PRDX3 protein (ab85559)
ab85559 on 15% SDS-PAGE (3μg).
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