AB87631

Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus)

Name: Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus) (ab87631) | Abcam
Brand: Abcam Limited
SKU: AB87631
Price: 370 USD
Availability: InStock

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(2 Publications)

Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB, FuncS.

View Alternative Names

AOP2, KIAA0106, PRDX6, Peroxiredoxin-6, 1-Cys peroxiredoxin, 24 kDa protein, Acidic calcium-independent phospholipase A2, Antioxidant protein 2, Glutathione-dependent peroxiredoxin, Liver 2D page spot 40, Lysophosphatidylcholine acyltransferase 5, Non-selenium glutathione peroxidase, Red blood cells page spot 12, 1-Cys PRX, aiPLA2, LPC acyltransferase 5, LPCAT-5, Lyso-PC acyltransferase 5, NSGPx

2 Images

Unknown

Western blot - Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus) (AB87631)

All lanes:

Western blot - Anti-Peroxiredoxin 6 antibody (<a href='/en-us/products/primary-antibodies/peroxiredoxin-6-antibody-ab73350'>ab73350</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus) (ab87631) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 25 kDa

true

Exposure time: 30s

SDS-PAGE - Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus) (AB87631)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus) (AB87631)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

false

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

FuncS, SDS-PAGE, WB

applications

Biologically active

Yes

Biological activity

Specific activity: approximately 95-120 pmole/min/μg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX6 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute Specific activity: approximately 95-120 pmole/min/μg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX6 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute.

Accession

P30041

Animal free

Carrier free

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"ab87631 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/peroxiredoxin-6-antibody-ab73350'>ab73350</a>." }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Specific activity: approximately 95-120 pmole/min/µg." } } }

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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P30041","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

True

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peroxiredoxin 6 also known as Prdx6 or peroxiredoxin-6 is a unique member of the peroxiredoxin family. It distinguishes itself by possessing both peroxidase and phospholipase A2 activities. This protein weighs approximately 25 kDa and is ubiquitously expressed in various tissues showing a high concentration in the lung. The expression pattern suggests its importance across different physiological contexts.

Biological function summary

We can understand peroxiredoxin 6 as a versatile enzyme that reduces hydrogen peroxide and organic hydroperoxides. It does not form a complex like some other peroxiredoxins. Instead it acts independently in cellular protection against oxidative stress. This role is mainly due to its peroxidase activity which helps in maintaining cellular redox balance and mitigating oxidative damage.

Pathways

Multiple cellular processes incorporate the enzymatic functions of peroxiredoxin 6. It plays an important role in the antioxidant defense pathway by collaborating with glutathione peroxidase to detoxify hydrogen peroxide. Another major pathway that involves Prdx6 is the phospholipid metabolism where it participates in the repair of oxidatively damaged cell membranes. This protein interacts with cytoplasmic thioredoxin proteins forming a network for managing intracellular reactive oxygen species.

Peroxiredoxin 6 has significant implications. Researchers have linked it to lung cancer where its expression levels correlate with disease progression possibly interacting with oncogenic proteins like NF-kB. In addition peroxiredoxin 6 is associated with neurodegenerative diseases such as Alzheimer’s disease. Its function in oxidative stress regulation might influence the aggregation of proteins like Tau which is a hallmark of Alzheimer's pathology.

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Specifications

Form

Liquid

Additional notes

Purified by using conventional chromatography.

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General info

Function

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively (PubMed : 10893423, PubMed : 9497358). Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides (PubMed : 10893423). Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity) (PubMed : 10893423, PubMed : 26830860). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH (PubMed : 10893423). Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis (PubMed : 10893423). Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC) (PubMed : 26830860). Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate (PubMed : 26830860).

Sequence similarities

Belongs to the peroxiredoxin family. Prx6 subfamily.

Post-translational modifications

Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress.. Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme (By similarity). The phosphorylated form exhibits a greater lysophosphatidylcholine acyltransferase activity compared to the non-phosphorylated form (PubMed:26830860).

Subcellular localisation

Lysosome

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PRDX6

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:15 PubMed39780184

2025

PRDX5 and PRDX6 translocation and oligomerization in bull sperm: a response to cryopreservation-induced oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Mostek-Majewska Agnieszka,Bossowska-Nowicka Magdalena,Słowińska Mariola,Ciereszko Andrzej

BMC genomics 24:245 PubMed37147584

2023

iTRAQ-based comparative proteomics reveal an enhancing role of PRDX6 in the freezability of Mediterranean buffalo sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Luo,Mingming Liang,Shihai Huang,Qingsong Xue,Xuan Ren,Yanfang Li,Jinli Wang,Deshun Shi,Xiangping Li

View all publications

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Product promise

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Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus)

Western blot - Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus) (AB87631)

SDS-PAGE - Recombinant human Peroxiredoxin 6 protein (His tag N-Terminus) (AB87631)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Biological activity

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

Additional notes

General info

Function

Sequence similarities

Post-translational modifications

Subcellular localisation

Product protocols

Target data

Publications (2)

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