Recombinant Human PFDN4 protein is a Human Full Length protein, in the 1 to 134 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A A T M K K A A A E D V N V T F E D Q Q K I N K F A R N T S R I T E L K E E I E V K K K Q L Q N L E D A C D D I M L A D D D C L M I P Y Q I G D V F I S H S Q E E T Q E M L E E A K K N L Q E E I D A L E S R V E S I Q R V L A D L K V Q L Y A K F G S N I N L E A D E S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Alternative names
PFD4, PFDN4, Prefoldin subunit 4, Protein C-1
Recommended products
Recombinant Human PFDN4 protein is a Human Full Length protein, in the 1 to 134 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NQP4-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.29% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A A T M K K A A A E D V N V T F E D Q Q K I N K F A R N T S R I T E L K E E I E V K K K Q L Q N L E D A C D D I M L A D D D C L M I P Y Q I G D V F I S H S Q E E T Q E M L E E A K K N L Q E E I D A L E S R V E S I Q R V L A D L K V Q L Y A K F G S N I N L E A D E S
- Accession
- Q9NQP4
- Protein length
- Full Length
- Predicted molecular weight
- 17.4 kDa
- Amino acids
- 1 to 134
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
- Sequence similarities
Belongs to the prefoldin subunit beta family.
- Subcellular localisation
- Nucleus, Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PFDN4 protein also known as Prefoldin Subunit 4 is part of a multi-subunit chaperone complex. The protein has a molecular mass of approximately 16 kDa and consists of several hydrophobic regions. Widely expressed in the cytoplasm PFDN4 interacts with actin and tubulin through its role in the prefoldin complex. It aids in the proper folding of these cytoskeletal proteins supporting overall cellular functions.
- Biological function summary
In cellular processes PFDN4 plays an important role as a component of the prefoldin complex. This complex is a heterohexameric assembly that supports the folding and transport of nascent polypeptide chains. Such activity is important for maintaining the stability and functionality of cytoskeletal proteins. By influencing protein conformation PFDN4 contributes to the structural integrity of cells.
- Pathways
In the protein biosynthesis context PFDN4 is involved in cytoskeleton organization and protein folding pathways. The prefoldin complex including PFDN4 facilitates the proper assembly and stabilization of tubulin which is significant for maintaining microtubule dynamics. Additionally PFDN4's interactions with chaperonin-containing TCP-1 (CCT) carry out essential roles in these pathways highlighting its contribution to cytoskeletal homeostasis.
- Associated diseases and disorders
Research links PFDN4 to conditions involving cytoskeletal defects such as certain neurodegenerative diseases and cancer. The malfunction of the prefoldin complex as seen in aberrant PFDN4 activity can disrupt cellular architecture and lead to disease progression. In particular PFDN4's relationship with proteins like tubulin and actin signifies that alterations in these interactions might contribute to oncogenesis and cancer metastasis.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human PFDN4 protein (ab103783)
15% SDS PAGE analysis of ab103783 (3 µg).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com