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AB103783

Recombinant Human PFDN4 protein

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Recombinant Human PFDN4 protein is a Human Full Length protein, in the 1 to 134 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PFD4, PFDN4, Prefoldin subunit 4, Protein C-1

1 Images
SDS-PAGE - Recombinant Human PFDN4 protein (AB103783)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PFDN4 protein (AB103783)

15% SDS PAGE analysis of ab103783 (3 µg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9NQP4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.29% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAATMKKAAAEDVNVTFEDQQKINKFARNTSRITELKEEIEVKKKQLQNLEDACDDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEEAKKNLQEEIDALESRVESIQRVLADLKVQLYAKFGSNINLEADES","proteinLength":"Full Length","predictedMolecularWeight":"17.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":134,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NQP4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PFDN4 protein also known as Prefoldin Subunit 4 is part of a multi-subunit chaperone complex. The protein has a molecular mass of approximately 16 kDa and consists of several hydrophobic regions. Widely expressed in the cytoplasm PFDN4 interacts with actin and tubulin through its role in the prefoldin complex. It aids in the proper folding of these cytoskeletal proteins supporting overall cellular functions.
Biological function summary

In cellular processes PFDN4 plays an important role as a component of the prefoldin complex. This complex is a heterohexameric assembly that supports the folding and transport of nascent polypeptide chains. Such activity is important for maintaining the stability and functionality of cytoskeletal proteins. By influencing protein conformation PFDN4 contributes to the structural integrity of cells.

Pathways

In the protein biosynthesis context PFDN4 is involved in cytoskeleton organization and protein folding pathways. The prefoldin complex including PFDN4 facilitates the proper assembly and stabilization of tubulin which is significant for maintaining microtubule dynamics. Additionally PFDN4's interactions with chaperonin-containing TCP-1 (CCT) carry out essential roles in these pathways highlighting its contribution to cytoskeletal homeostasis.

Research links PFDN4 to conditions involving cytoskeletal defects such as certain neurodegenerative diseases and cancer. The malfunction of the prefoldin complex as seen in aberrant PFDN4 activity can disrupt cellular architecture and lead to disease progression. In particular PFDN4's relationship with proteins like tubulin and actin signifies that alterations in these interactions might contribute to oncogenesis and cancer metastasis.
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Specifications

Form

Liquid

Additional notes

purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Function

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

Sequence similarities

Belongs to the prefoldin subunit beta family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
See full target information PFDN4
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