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AB132823

Recombinant Human PFKP protein (GST tag N-Terminus)

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Recombinant Human PFKP protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 784 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

PFKF, PFKP, ATP-PFK, PFK-P, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, Phosphohexokinase, PFK-C

3 Images
Western blot - Recombinant Human PFKP protein (GST tag N-Terminus) (AB132823)
  • WB

Lab

Western blot - Recombinant Human PFKP protein (GST tag N-Terminus) (AB132823)

Blocking/Dilution buffer : 5% NFDM/TBST. Exposure times : Lane 1 : 3 seconds; Lane 2 : 10 seconds; Lane 3 : 180 seconds.

All lanes:

Western blot - Anti-PFKM antibody [EPR10734(B)] (<a href='/en-us/products/primary-antibodies/pfkm-antibody-epr10734b-ab154804'>ab154804</a>) at 1/1000 dilution

Lane 1:

DDK tagged Recombinant Human PFKM protein (Full length, 85 KDa)

Lane 2:

Western blot - Recombinant Human PFKP protein (GST tag N-Terminus) (ab132823)

Lane 3:

GST tagged Recombinant Human PFKL protein (Full length, 116 KDa)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 85 kDa

Observed band size: 85 kDa

false

Western blot - Recombinant Human PFKP protein (GST tag N-Terminus) (AB132823)
  • WB

Supplier Data

Western blot - Recombinant Human PFKP protein (GST tag N-Terminus) (AB132823)

Blocking/diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane1 : 10 seconds Lane 2 : 1 seconds

All lanes:

Western blot - Anti-PFKM + PFKP antibody [EPR17314] (<a href='/en-us/products/primary-antibodies/pfkm-pfkp-antibody-epr17314-ab204131'>ab204131</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human PFKP protein (GST tag N-Terminus) (ab132823)

Lane 2:

DDK tagged Recombinant Human PFKM protein (Full length, 85 KDa)

Secondary

Lanes 1 - 2:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

SDS-PAGE - Recombinant Human PFKP protein (GST tag N-Terminus) (AB132823)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PFKP protein (GST tag N-Terminus) (AB132823)

12.5% SDS-PAGE analysis of ab132823 stained with Coomassie Blue

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

Q01813

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris-HCl buffer, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB204131

Anti-PFKM + PFKP antibody [EPR17314]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PFKM + PFKP antibody [EPR17314] (AB204131)

  • 0
  • 0
Assay Kits

AB155898

Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric)

Functional Studies - Phosphofructokinase Activity Assay Kit (6-PFK, Colorimetric) (AB155898)

  • 0
  • 0
Assay Kits

AB83432

Pyruvate Kinase Assay Kit

Functional Studies - Pyruvate Kinase Assay Kit (AB83432)

  • 0
  • 0
Assay Kits

AB241024

Enolase Assay Kit

Functional Studies - Enolase Assay Kit (AB241024)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMGIYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQAFRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELARNGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDAIMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEEQMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDTRVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPDTPACVVSLNGNHAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIKLPDDQIPKTNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGFDGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSINALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSIGADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGADAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFIYQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAKLKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQWWLKLRPLMKILAKYKASYDVSDSGQLEHVQPWSV","proteinLength":"Full Length","predictedMolecularWeight":"111.98 kDa","actualMolecularWeight":null,"aminoAcidEnd":784,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q01813","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFKP also known as 6-phosphofructokinase platelet type functions as an important glycolytic enzyme that catalyzes the phosphorylation of fructose 6-phosphate to fructose 16-bisphosphate using ATP. This process shows the rate-limiting step in glycolysis. The PFKP protein weighs approximately 85 kDa. Expression occurs in various tissues with high presence in platelets and muscle tissues indicating its significant role in energy metabolism.
Biological function summary

PFKP plays an essential role in maintaining energy supply ensuring efficient glycolysis during high energy demands. It associates with other isoforms PFKM (muscle) and PFKL (liver) to form a tetrameric complex which can adapt its activity based on the energy needs of the cell. This adaptability defines its contribution to cellular metabolism regulation considering energy substrates and signaling molecules.

Pathways

The function of PFKP ties closely to glycolysis and the larger metabolic pathways managing energy production such as the tricarboxylic acid (TCA) cycle. Within these pathways PFKP interacts with other metabolic regulators including AMP-activated protein kinase (AMPK) which influences cell metabolism in response to energy availability. Connections with PFKL and PFKM reveal that PFKP's activity relates to the overall glycolytic flux and energy balance.

PFKP's alterations contribute to metabolic conditions like cancer and diabetes. Its dysregulation in cancer cells supports the Warburg effect where tumor cells prefer glycolysis. The link with metabolic proteins such as AMPK also indicates a potential role in diabetes as both disorders involve shifts in energy metabolism. Understanding PFKP's impact on these diseases helps in developing therapeutic strategies targeting metabolic pathways.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.

Post-translational modifications

Phosphorylation at Ser-386 promotes interaction with ATG4B.. GlcNAcylation decreases enzyme activity.

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Product protocols

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Target data

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
See full target information PFKP
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