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AB177576

Recombinant human PFTK1 (CDK14)/CyclinY protein

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Recombinant human PFTK1 (CDK14)/CyclinY protein is a Human Full Length protein, in the 1 to 469 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA0834, PFTK1, CDK14, Cyclin-dependent kinase 14, Cell division protein kinase 14, Serine/threonine-protein kinase PFTAIRE-1, hPFTAIRE1

4 Images
Functional Studies - Recombinant human PFTK1 (CDK14)/CyclinY protein (AB177576)
  • FuncS

Supplier Data

Functional Studies - Recombinant human PFTK1 (CDK14)/CyclinY protein (AB177576)

The specific activity of ab177576 was determined to be 2.5 nmol/min/mg.

Functional Studies - Recombinant human PFTK1 (CDK14)/CyclinY protein (AB177576)
  • FuncS

Unknown

Functional Studies - Recombinant human PFTK1 (CDK14)/CyclinY protein (AB177576)

The specific activity of PFTK1 (CDK14)/CyclinY (ab177576) was determined to be 8.3 nmol/min/mg as per activity assay protocol and was equivalent to 2.7 nmol/min/mg as per radiometric assay

SDS-PAGE - Recombinant human PFTK1 (CDK14)/CyclinY protein (AB177576)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human PFTK1 (CDK14)/CyclinY protein (AB177576)

SDS PAGE analysis of ab177576

SDS-PAGE - Recombinant human PFTK1 (CDK14)/CyclinY protein (AB177576)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PFTK1 (CDK14)/CyclinY protein (AB177576)

SDS-PAGE analysis of ab177576.

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The specific activity of ab177576 was determined to be 2.5 nmol/min/mg

Accession

O94921

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab56270 (Human Rb protein fragment), ab89813 (Histone H1 protein) and ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Sequence info

[{"linker":null,"sequence":"MCDLIEPQPAEKIGKMKKLRRTLSESFSRIALKKDDTTFDEICVTKMSTRNCQGMDSVIKPLDTIPEDKKVRVQRTQSTFDPFEKPANQVKRVHSENNACINFKTSSTGKESPKVRRHSSPSSPTSPKFGKADSYEKLEKLGEGSYATVYKGKSKVNGKLVALKVIRLQEEEGTPFTAIREASLLKGLKHANIVLLHDIIHTKETLTLVFEYVHTDLCQYMDKHPGGLHPDNVKLFLFQLLRGLSYIHQRYILHRDLKPQNLLISDTGELKLADFGLARAKSVPSHTYSNEVVTLWYRPPDVLLGSTEYSTCLDMWGVGCIFVEMIQGVAAFPGMKDIQDQLERIFLVLGTPNEDTWPGVHSLPHFKPERFTLYSSKNLRQAWNKLSYVNHAEDLASKLLQCSPKNRLSAQAALSHEYFSDLPPRLWELTDMSSIFTVPNVRLQPEAGESMRAFGKNNSYGKSLSNSKH","proteinLength":"Full Length","predictedMolecularWeight":"72 kDa","actualMolecularWeight":null,"aminoAcidEnd":469,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O94921","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFTK1 also known as CDK14 is a serine/threonine protein kinase that associates with Cyclin Y. This protein has a molecular mass of approximately 58 kDa. PFTK1 expresses in various tissues with notable expression in the brain and testis. It plays a role in cell cycle control particularly in regulating the G2/M transition phase contributing to cellular proliferation and differentiation processes.
Biological function summary

PFTK1 interacts with Cyclin Y to form a functional complex that activates downstream signaling pathways. This complex is integral in driving proper cell cycle progression and has been implicated in regulating gene transcription. Cyclin Y acts as a regulatory subunit that controls PFTK1 activity ensuring precise timing and direction of cellular processes. This ability to modulate cellular functions links PFTK1 to additional pathways involved in cellular growth.

Pathways

PFTK1's role in cellular growth is highlighted by its involvement in the Wnt signaling and cell cycle pathways. In the Wnt signaling pathway PFTK1 associates with proteins such as β-catenin influencing cellular proliferation and differentiation. In the context of cell cycle pathways CDKs (cyclin-dependent kinases) that interact with Cyclin Y further contribute to phase transitions coordinating with proteins like cyclin D to oversee orderly cell cycle completion.

PFTK1 has been associated with cancer and neurodegenerative diseases. Research suggests that overexpression or misregulation of PFTK1 contributes to tumorigenesis with links to proteins such as p53 a tumor suppressor involved in cell cycle arrest. In neurodegenerative diseases alterations in PFTK1 expression may affect signaling pathways leading to neuronal death where interactions with proteins such as tau provide insight into disease mechanisms. Understanding PFTK1's role in these conditions aids in developing potential therapeutic strategies.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.
See full target information CDK14
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