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AB167928

Recombinant Human PFTK1 protein

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(1 Publication)

Recombinant Human PFTK1 protein is a Human Full Length protein, in the 1 to 451 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

KIAA0834, PFTK1, CDK14, Cyclin-dependent kinase 14, Cell division protein kinase 14, Serine/threonine-protein kinase PFTAIRE-1, hPFTAIRE1

1 Images
SDS-PAGE - Recombinant Human PFTK1 protein (AB167928)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PFTK1 protein (AB167928)

SDS-PAGE analysis of ab167928.

Key facts

Purity

>95% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

O94921

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Primary Antibodies

AB224098

Anti-PFTK1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (AB224098)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB181225

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

  • 0
  • 0
Primary Antibodies

AB171084

Anti-Mad2L1 antibody [EPR9852]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Mad2L1 antibody [EPR9852] (AB171084)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MHGYFGCNAAAEPGYSAFVGTPQICVTKMSTRNCQGMDSVIKPLDTIPEDKKVRVQRTQSTFDPFEKPANQVKRVHSENNACINFKTSSTGKESPKVRRHSSPSSPTSPKFGKADSYEKLEKLGEGSYATVYKGKSKVNGKLVALKVIRLQEEEGTPFTAIREASLLKGLKHANIVLLHDIIHTKETLTLVFEYVHTDLCQYMDKHPGGLHPDNVKLFLFQLLRGLSYIHQRYILHRDLKPQNLLISDTGELKLADFGLARAKSVPSHTYSNEVVTLWYRPPDVLLGSTEYSTCLDMWGVGCIFVEMIQGVAAFPGMKDIQDQLERIFLVLGTPNEDTWPGVHSLPHFKPERFTLYSSKNLRQAWNKLSYVNHAEDLASKLLQCSPKNRLSAQAALSHEYFSDLPPRLWELTDMSSIFTVPNVRLQPEAGESMRAFGKNNSYGKSLSNSKH","proteinLength":"Full Length","predictedMolecularWeight":"72 kDa","actualMolecularWeight":null,"aminoAcidEnd":451,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"O94921","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PFTK1 also known as PFTAIRE protein kinase 1 or CDK14 belongs to the cyclin-dependent kinase family. It has a molecular mass of approximately 36 kDa. PFTK1 is expressed in various tissues with high levels in the brain and testis. This kinase plays a role in cell cycle regulation by phosphorylating specific substrates influencing cellular processes related to proliferation and growth.
Biological function summary

PFTK1 regulates the cell cycle particularly during the G2/M transition. It does not form traditional cyclin-CDK complexes which sets it apart from other known CDKs. Instead PFTK1's activity relies on its interaction with a range of non-cyclin proteins. By aiding cell cycle progression PFTK1 impacts cellular differentiation and neuronal development which highlights its importance in neural tissues.

Pathways

PFTK1 participates in the Wnt signaling pathway and the neuronal differentiation pathway. In the Wnt pathway PFTK1 interacts with proteins that modulate beta-catenin signaling such as GSK-3β. These interactions influence transcriptional activity and cellular proliferation. The kinase's involvement in neuronal differentiation suggests its importance in neural precursor development and maturation.

PFTK1 has connections to cancer and neurodegenerative diseases. PFTK1 overexpression is often observed in cancers like gliomas suggesting its role in tumorigenesis. PFTK1 associates with Cyclin D3 in some cancers influencing tumor growth and progression. Furthermore altered PFTK1 activity impacts neuronal survival and function linking it to disorders such as Alzheimer's disease.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport.
See full target information CDK14
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of surgical oncology 18:147 PubMed32605563

2020

Upregulation of miR-1825 inhibits the progression of glioblastoma by suppressing CDK14 though Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fengqin Lu,Chunhong Li,Yuping Sun,Ting Jia,Na Li,Haiyan Li
View all publications
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