Recombinant Human PGCP protein is a Human Full Length protein, in the 1 to 472 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M K F L I F A F F G G V H L L S L C S G K A I C K N G I S K R T F E E I K E E I A S C G D V A K A I I N L A V Y G K A Q N R S Y E R L A L L V D T V G P R L S G S K N L E K A I Q I M Y Q N L Q Q D G L E K V H L E P V R I P H W E R G E E S A V M L E P R I H K I A I L G L G S S I G T P P E G I T A E V L V V T S F D E L Q R R A S E A R G K I V V Y N Q P Y I N Y S R T V Q Y R T Q G A V E A A K V G A L A S L I R S V A S F S I Y S P H T G I Q E Y Q D G V P K I P T A C I T V E D A E M M S R M A S H G I K I V I Q L K M G A K T Y P D T D S F N T V A E I T G S K Y P E Q V V L V S G H L D S W D V G Q G A M D D G G G A F I S W E A L S L I K D L G L R P K R T L R L V L W T A E E Q G G V G A F Q Y Y Q L H K V N I S N Y S L V M E S D A G T F L P T G L Q F T G S E K A R A I M E E V M S L L Q P L N I T Q V L S H G E G T D I N F W I Q A G V P G A S L L D D L Y K Y F F F H H S H G D T M T V M D P K Q M N V A A A V W A V V S Y V V A D M E E M L P R S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Carboxypeptidase that may play an important role in the hydrolysis of circulating peptides. Catalyzes the hydrolysis of dipeptides with unsubstituted terminals into amino acids. May play a role in the liberation of thyroxine hormone from its thyroglobulin (Tg) precursor.
Alternative names
LCH1, PGCP, CPQ, Carboxypeptidase Q, Lysosomal dipeptidase, Plasma glutamate carboxypeptidase
Recommended products
Recombinant Human PGCP protein is a Human Full Length protein, in the 1 to 472 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9Y646-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M K F L I F A F F G G V H L L S L C S G K A I C K N G I S K R T F E E I K E E I A S C G D V A K A I I N L A V Y G K A Q N R S Y E R L A L L V D T V G P R L S G S K N L E K A I Q I M Y Q N L Q Q D G L E K V H L E P V R I P H W E R G E E S A V M L E P R I H K I A I L G L G S S I G T P P E G I T A E V L V V T S F D E L Q R R A S E A R G K I V V Y N Q P Y I N Y S R T V Q Y R T Q G A V E A A K V G A L A S L I R S V A S F S I Y S P H T G I Q E Y Q D G V P K I P T A C I T V E D A E M M S R M A S H G I K I V I Q L K M G A K T Y P D T D S F N T V A E I T G S K Y P E Q V V L V S G H L D S W D V G Q G A M D D G G G A F I S W E A L S L I K D L G L R P K R T L R L V L W T A E E Q G G V G A F Q Y Y Q L H K V N I S N Y S L V M E S D A G T F L P T G L Q F T G S E K A R A I M E E V M S L L Q P L N I T Q V L S H G E G T D I N F W I Q A G V P G A S L L D D L Y K Y F F F H H S H G D T M T V M D P K Q M N V A A A V W A V V S Y V V A D M E E M L P R S
- Accession
- Q9Y646
- Protein length
- Full Length
- Amino acids
- 1 to 472
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Carboxypeptidase that may play an important role in the hydrolysis of circulating peptides. Catalyzes the hydrolysis of dipeptides with unsubstituted terminals into amino acids. May play a role in the liberation of thyroxine hormone from its thyroglobulin (Tg) precursor.
- Sequence similarities
Belongs to the peptidase M28 family.
- Post-translational modifications
N-glycosylated. The secreted form is modified by hybrid or complex type oligosaccharide chains (By similarity).
- Subcellular localisation
- Lysosome
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PGCP also known as plasma glutamate carboxypeptidase functions mechanically by catalyzing the cleavage of C-terminal glutamate residues from its substrates. PGCP with a molecular mass approximately 55 kDa is highly expressed in the liver and kidney. Researchers have observed its expression in both human and mouse tissues which implies a conserved role across species. PGCP participates in processes that maintain proper levels of free glutamate an important amino acid in metabolic pathways.
- Biological function summary
PGCP modulates the balance of circulating peptides by hydrolyzing carboxy-terminal glutamate. It does not operate as part of a larger protein complex but functions independently. By regulating peptides PGCP helps in processes like protein degradation and recycling. It also influences the bioavailability of certain hormones and metabolites thereby affecting metabolic and signal transduction pathways.
- Pathways
PGCP actively partakes in the regulation of blood coagulation and metabolism of amino acids. It interacts with proteins like angiotensin II as it processes bioactive peptides involved in blood pressure regulation. Through these actions PGCP affects the renin-angiotensin system which plays a critical role in cardiovascular health. Additionally PGCP associates with amino acid metabolism pathways where its role in glutamate modulation supports cellular energy production and neurotransmitter synthesis.
- Associated diseases and disorders
PGCP's dysregulation connects it to hypertension and metabolic syndrome. Researchers link altered PGCP activity with imbalances in the renin-angiotensin system leading to increased blood pressure. Furthermore its interaction with proteins like bradykinin influences inflammatory responses and researchers hypothesize its involvement in metabolic syndrome due to its role in amino acid processing. Understanding PGCP's contributions aids in exploring therapeutic interventions for these conditions.
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1 product image
SDS-PAGE - Recombinant Human PGCP protein (ab160974)
ab160974 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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