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AB208326

Recombinant human PGD protein (His tag N-Terminus)

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Recombinant human PGD protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 483 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

PGDH, PGD

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SDS-PAGE - Recombinant human PGD protein (His tag N-Terminus) (AB208326)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human PGD protein (His tag N-Terminus) (AB208326)

15% SDS-PAGE analysis of ab208326 (3 μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 10 units/mg, in which one unit oxidize 1.0 μmole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25°C, in the presence of β-NADP.

Accession

P52209

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAQADIALIGLAVMGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAQSLKEMVSKLKKPRRIILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRDLKAKGILFVGSGVSGGEEGARYGPSLMPGGNKEAWPHIKTIFQGIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLGMAQDEMAQAFEDWNKTELDSFLIEITANILKFQDTDGKHLLPKIRDSAGQKGTGKWTAISALEYGVPVTLIGEAVFARCLSSLKDERIQASKKLKGPQKFQFDGDKKSFLEDIRKALYASKIISYAQGFMLLRQAATEFGWTLNYGGIALMWRGGCIIRSVFLGKIKDAFDRNPELQNLLLDDFFKSAVENCQDSWRRAVSTGVQAGIPMPCFTTALSFYDGYRHEMLPASLIQAQRDYFGAHTYELLAKPGQFIHTNWTGHGGTVSSSSYNA","proteinLength":"Full Length","predictedMolecularWeight":"55.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":483,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P52209","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGD also known as 6-phosphogluconate dehydrogenase is an enzyme with a mass of approximately 53 kDa. It plays an important role in catalyzing the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate producing NADPH in the process. PGD expression occurs in various tissues with notably high levels in liver and red blood cells (RBCs). Alternative names include 6PGD and phosphogluconate dehydrogenase with its corresponding gene expressed across eukaryotic organisms.
Biological function summary

The enzyme 6-phosphogluconate dehydrogenase is essential in cellular metabolism and redox homeostasis. It functions as part of the pentose phosphate pathway a metabolic route critical for the generation of NADPH and ribose 5-phosphate. NADPH is necessary for biosynthetic reactions and in protecting cells against oxidative stress. 6PGD operates as a homodimer and is not part of a larger protein complex enabling it to perform its functions independently within the cell.

Pathways

PGD stands as a major component of the pentose phosphate pathway alongside glucose-6-phosphate dehydrogenase (G6PD). This pathway is significant for providing reducing power and pentose phosphates for anabolic reactions. Both 6-phosphogluconate and G6PD assist in the proper functioning of this pathway facilitating the balance of NADP+/NADPH. The pathway intricately connects to glycolysis enabling the cells to adapt to fluctuations in metabolic demands through intermediates that interconvert between these pathways.

Altered activity of 6-phosphogluconate dehydrogenase links to genetic disorders like hemolytic anemia. The pentose phosphate pathway's impairment particularly due to deficiency in PGD or its associated protein G6PD exacerbates anemia under oxidative stress. Additionally PGD overexpression has associations with many cancers as elevated NADPH production supports tumor survival and resistance. Understanding the role of PGD in these conditions helps in recognizing the importance of this enzyme in maintaining cellular health and adapting to disease challenges.
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Specifications

Form

Liquid

Additional notes

ab208326 purified by using conventional chromatography techniques.

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General info

Function

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.

Sequence similarities

Belongs to the 6-phosphogluconate dehydrogenase family.

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Product protocols

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Target data

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
See full target information PGD
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