Recombinant Human PGD protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A Q A D I A L I G L A V M G Q N L I L N M N D H G F V V C A F N R T V S K V D D F L A N E A K G T K V V G A Q S L K E M V S K L K K P R R I I L L V K A G Q A V D D F I E K L V P L L D T G D I I I D G G N S E Y R D T T R R C R D L K A K G I L F V G S G V S G G E E G A R Y G P S L M P G G N K E A W P H I K T I F Q G I A A K V G T G E P C C D W V G D E G A G H F V K M V H N G I E Y G D M Q L I C E A Y H L M K D V L G M A Q D E M A Q A F E D W N K T E L D S F L I E I T A N I L K F Q D T D G K H L L P K I R D S A G Q K G T G K W T A I S A L E Y G V P V T L I G E A V F A R C L S S L K D E R I Q A S K K L K G P Q K F Q F D G D K K S F L E D I R K A L Y A S K I I S Y A Q G F M L L R Q A A T E F G W T L N Y G G I A L M W R G G C I I R S V F L G K I K D A F D R N P E L Q N L L L D D F F K S A V E N C Q D S W R R A V S T G V Q A G I P M P C F T T A L S F Y D G Y R H E M L P A S L I Q A Q R D Y F G A H T Y E L L A K P G Q F I H T N W T G H G G T V S S S S Y N A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
Alternative names
PGDH, PGD
Recommended products
Recombinant Human PGD protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P52209-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A Q A D I A L I G L A V M G Q N L I L N M N D H G F V V C A F N R T V S K V D D F L A N E A K G T K V V G A Q S L K E M V S K L K K P R R I I L L V K A G Q A V D D F I E K L V P L L D T G D I I I D G G N S E Y R D T T R R C R D L K A K G I L F V G S G V S G G E E G A R Y G P S L M P G G N K E A W P H I K T I F Q G I A A K V G T G E P C C D W V G D E G A G H F V K M V H N G I E Y G D M Q L I C E A Y H L M K D V L G M A Q D E M A Q A F E D W N K T E L D S F L I E I T A N I L K F Q D T D G K H L L P K I R D S A G Q K G T G K W T A I S A L E Y G V P V T L I G E A V F A R C L S S L K D E R I Q A S K K L K G P Q K F Q F D G D K K S F L E D I R K A L Y A S K I I S Y A Q G F M L L R Q A A T E F G W T L N Y G G I A L M W R G G C I I R S V F L G K I K D A F D R N P E L Q N L L L D D F F K S A V E N C Q D S W R R A V S T G V Q A G I P M P C F T T A L S F Y D G Y R H E M L P A S L I Q A Q R D Y F G A H T Y E L L A K P G Q F I H T N W T G H G G T V S S S S Y N A
- Accession
- P52209
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab93633 is purified using conventional chromatography techniques.
General info
- Function
Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.
- Sequence similarities
Belongs to the 6-phosphogluconate dehydrogenase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PGD also known as 6-phosphogluconate dehydrogenase is an enzyme with a mass of approximately 53 kDa. It plays an important role in catalyzing the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate producing NADPH in the process. PGD expression occurs in various tissues with notably high levels in liver and red blood cells (RBCs). Alternative names include 6PGD and phosphogluconate dehydrogenase with its corresponding gene expressed across eukaryotic organisms.
- Biological function summary
The enzyme 6-phosphogluconate dehydrogenase is essential in cellular metabolism and redox homeostasis. It functions as part of the pentose phosphate pathway a metabolic route critical for the generation of NADPH and ribose 5-phosphate. NADPH is necessary for biosynthetic reactions and in protecting cells against oxidative stress. 6PGD operates as a homodimer and is not part of a larger protein complex enabling it to perform its functions independently within the cell.
- Pathways
PGD stands as a major component of the pentose phosphate pathway alongside glucose-6-phosphate dehydrogenase (G6PD). This pathway is significant for providing reducing power and pentose phosphates for anabolic reactions. Both 6-phosphogluconate and G6PD assist in the proper functioning of this pathway facilitating the balance of NADP+/NADPH. The pathway intricately connects to glycolysis enabling the cells to adapt to fluctuations in metabolic demands through intermediates that interconvert between these pathways.
- Associated diseases and disorders
Altered activity of 6-phosphogluconate dehydrogenase links to genetic disorders like hemolytic anemia. The pentose phosphate pathway's impairment particularly due to deficiency in PGD or its associated protein G6PD exacerbates anemia under oxidative stress. Additionally PGD overexpression has associations with many cancers as elevated NADPH production supports tumor survival and resistance. Understanding the role of PGD in these conditions helps in recognizing the importance of this enzyme in maintaining cellular health and adapting to disease challenges.
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1 product image
SDS-PAGE - Recombinant Human PGD protein (ab93633)
15% SDS-PAGE showing ab93633 at approximately 55.3kDa (3μg).
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