Recombinant human PGK1 protein is a Human Full Length protein, in the 1 to 417 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and mass spectrometry.The predicted molecular weight of ab211320 protein is 46.8 kDa.
- Save time and ensure accurate results - use our recombinant Human Phosphoglycerate kinase-1 (PGK-1/ PGKA) protein as a control
- Optimal protein bioactivity and stability
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Publications
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- Neoplasia (New York, N.Y.) 21:893-9072019A Novel Pyrazolopyrimidine Ligand of Human PGK1 and Stress Sensor DJ1 Modulates the Shelterin Complex and Telomere Length Regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlan E Bilsland et. al.PubMed 31401411
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S L S N K L T L D K L D V K G K R V V M R V D F N V P M K N N Q I T N N Q R I K A A V P S I K F C L D N G A K S V V L M S H L G R P D G V P M P D K Y S L E P V A V E L K S L L G K D V L F L K D C V G P E V E K A C A N P A A G S V I L L E N L R F H V E E E G K G K D A S G N K V K A E P A K I E A F R A S L S K L G D V Y V N D A F G T A H R A H S S M V G V N L P Q K A G G F L M K K E L N Y F A K A L E S P E R P F L A I L G G A K V A D K I Q L I N N M L D K V N E M I I G G G M A F T F L K V L N N M E I G T S L F D E E G A K I V K D L M S K A E K N G V K I T L P V D F V T A D K F D E N A K T G Q A T V A S G I P A G W M G L D C G P E S S K K Y A E A V T R A K Q I V W N G P V G V F E W E A F A R G T K A L M D E V V K A T S R G C I T I I G G G D T A T C C A K W N T E D K V S H V S T G G G A S L E L L E G K V L P G V D A L S N I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
Alternative names
PGKA, MIG10, OK/SW-cl.110, PGK1, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2
Recommended products
Recombinant human PGK1 protein is a Human Full Length protein, in the 1 to 417 aa range with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and mass spectrometry.The predicted molecular weight of ab211320 protein is 46.8 kDa.
- Save time and ensure accurate results - use our recombinant Human Phosphoglycerate kinase-1 (PGK-1/ PGKA) protein as a control
- Optimal protein bioactivity and stability
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS, MS
- Biologically active
- Yes
- Biological activity
- Specific activity: > 600 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37°C.
- Accession
- P00558-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S L S N K L T L D K L D V K G K R V V M R V D F N V P M K N N Q I T N N Q R I K A A V P S I K F C L D N G A K S V V L M S H L G R P D G V P M P D K Y S L E P V A V E L K S L L G K D V L F L K D C V G P E V E K A C A N P A A G S V I L L E N L R F H V E E E G K G K D A S G N K V K A E P A K I E A F R A S L S K L G D V Y V N D A F G T A H R A H S S M V G V N L P Q K A G G F L M K K E L N Y F A K A L E S P E R P F L A I L G G A K V A D K I Q L I N N M L D K V N E M I I G G G M A F T F L K V L N N M E I G T S L F D E E G A K I V K D L M S K A E K N G V K I T L P V D F V T A D K F D E N A K T G Q A T V A S G I P A G W M G L D C G P E S S K K Y A E A V T R A K Q I V W N G P V G V F E W E A F A R G T K A L M D E V V K A T S R G C I T I I G G G D T A T C C A K W N T E D K V S H V S T G G G A S L E L L E G K V L P G V D A L S N I
- Accession
- P00558
- Protein length
- Full Length
- Predicted molecular weight
- 46.8 kDa
- Amino acids
- 1 to 417
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab211320 was purified using conventional chromatography techniques.
General info
- Function
Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
- Sequence similarities
Belongs to the phosphoglycerate kinase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our bioactive recombinant human PGK-1/ PGKA as a control in SDS-PAGE and mass spectrometry.
Check out our protein gel staining guide for SDS-PAGE here
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PGK1 also known as phosphoglycerate kinase-1 is an enzyme involved in glycolysis with a mass of approximately 45 kDa. PGK1 catalyzes the reversible transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP. This enzyme is expressed ubiquitously across various tissues with significant roles in energy metabolism. In yeast it is referred to simply as phosphoglycerate kinase where its function in cellular respiration is widely studied.
- Biological function summary
The PGK1 protein plays an essential role in energy production by participating in the glycolytic pathway. This enzyme functions independently and is not part of any complex. By converting 13-bisphosphoglycerate to 3-phosphoglycerate it supports cellular processes that require ATP. Such activity facilitates the high energy needs of cells ensuring proper cellular function and homeostasis.
- Pathways
The enzyme PGK1 operates predominantly within the glycolytic and gluconeogenesis pathways. Throughout glycolysis it partners with enzymes like phosphofructokinase and pyruvate kinase facilitating the breakdown of glucose into pyruvate while generating ATP. In gluconeogenesis PGK1 aids in glucose formation which helps balance energy dynamics particularly in tissues like the liver and kidney.
- Associated diseases and disorders
PGK1 has associations with tumor growth and proliferation in various cancers. Its elevated expression is often found in tumors linking it to oncogenesis due to the increased demand for glycolytic activity. Additionally mutations in PGK1 can result in phosphoglycerate kinase deficiency a disorder affecting red blood cell metabolism. This links it to related proteins involved in managing metabolic pathways of energy production in affected tissues.
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1 product image
SDS-PAGE - Recombinant human PGK1 protein (ab211320)
15% SDS-PAGE analysis of ab211320 (3μg).
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