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Recombinant human PGK1 protein is a Human Full Length protein, in the 1 to 417 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS.

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Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS, MS

Biologically active

Yes

Sequence

M G S S H H H H H H S S G L V P R G S H M S L S N K L T L D K L D V K G K R V V M R V D F N V P M K N N Q I T N N Q R I K A A V P S I K F C L D N G A K S V V L M S H L G R P D G V P M P D K Y S L E P V A V E L K S L L G K D V L F L K D C V G P E V E K A C A N P A A G S V I L L E N L R F H V E E E G K G K D A S G N K V K A E P A K I E A F R A S L S K L G D V Y V N D A F G T A H R A H S S M V G V N L P Q K A G G F L M K K E L N Y F A K A L E S P E R P F L A I L G G A K V A D K I Q L I N N M L D K V N E M I I G G G M A F T F L K V L N N M E I G T S L F D E E G A K I V K D L M S K A E K N G V K I T L P V D F V T A D K F D E N A K T G Q A T V A S G I P A G W M G L D C G P E S S K K Y A E A V T R A K Q I V W N G P V G V F E W E A F A R G T K A L M D E V V K A T S R G C I T I I G G G D T A T C C A K W N T E D K V S H V S T G G G A S L E L L E G K V L P G V D A L S N I

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Shipping To:
United States

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
MS
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

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Recombinant human PGK1 protein is a Human Full Length protein, in the 1 to 417 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Applications

SDS-PAGE, FuncS, MS

Biological activity

Specific activity: > 600 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37°C.

Predicted molecular weight

46.8 kDa

Amino acids

1 to 417

Sequence

M G S S H H H H H H S S G L V P R G S H M S L S N K L T L D K L D V K G K R V V M R V D F N V P M K N N Q I T N N Q R I K A A V P S I K F C L D N G A K S V V L M S H L G R P D G V P M P D K Y S L E P V A V E L K S L L G K D V L F L K D C V G P E V E K A C A N P A A G S V I L L E N L R F H V E E E G K G K D A S G N K V K A E P A K I E A F R A S L S K L G D V Y V N D A F G T A H R A H S S M V G V N L P Q K A G G F L M K K E L N Y F A K A L E S P E R P F L A I L G G A K V A D K I Q L I N N M L D K V N E M I I G G G M A F T F L K V L N N M E I G T S L F D E E G A K I V K D L M S K A E K N G V K I T L P V D F V T A D K F D E N A K T G Q A T V A S G I P A G W M G L D C G P E S S K K Y A E A V T R A K Q I V W N G P V G V F E W E A F A R G T K A L M D E V V K A T S R G C I T I I G G G D T A T C C A K W N T E D K V S H V S T G G G A S L E L L E G K V L P G V D A L S N I

Accession
P00558-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Concentration
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Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Specifications

Form

Liquid

Additional notes

ab211320 was purified using conventional chromatography techniques.

General info

Function

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed:30323285, PubMed:7391028). In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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