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AB123166

Recombinant Human PGK2 protein

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(2 Publications)

Recombinant Human PGK2 protein is a Human Full Length protein, in the 1 to 417 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PGKB, PGK2, Phosphoglycerate kinase 2

1 Images
SDS-PAGE - Recombinant Human PGK2 protein (AB123166)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PGK2 protein (AB123166)

15% SDS-PAGE showing ab123166 (3µg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P07205

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVLMSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVEALSNM","proteinLength":"Full Length","predictedMolecularWeight":"46.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":417,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P07205","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoglycerate kinase 2 (PGK2) also known as testis-specific phosphoglycerate kinase is an enzyme with a molecular mass of approximately 45 kDa. It functions in glycolysis by catalyzing the reversible conversion of 13-bisphosphoglycerate to 3-phosphoglycerate producing ATP in the process. PGK2 expression is restricted mostly to the testes where it plays an important role in sperm development and motility. Unlike its isozyme counterpart PGK1 which is expressed ubiquitously PGK2 is testis-specific.
Biological function summary

The enzyme participates in the important processes of energy production and spermatozoa development. While PGK2 does not form part of any multi-protein complex it is an integral component of the glycolytic pathway contributing to ATP production necessary for sperm motility. Its expression is regulated during spermatogenesis highlighting its role in male fertility.

Pathways

PGK2 operates within the glycolytic pathway a major metabolic route for energy production. Along with glyceraldehyde-3-phosphate dehydrogenase PGK2 catalyzes important reactions that facilitate the conversion of glucose to pyruvate yielding high-energy ATP molecules. By functioning in tandem with other glycolytic enzymes PGK2 ensures the efficient production of energy needed for cellular functions especially during spermatogenesis.

Defects or alterations in PGK2 have associations with male infertility notably due to impaired sperm function. Disorders like spermatogenic failure and related conditions arise when PGK2 activity diminishes. Its enzyme partner glyceraldehyde-3-phosphate dehydrogenase-S is also linked to sperm motility issues. Understanding PGK2’s role aids in identifying therapeutic targets to address such reproductive challenges.
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Specifications

Form

Liquid

Additional notes

ab123166 is purified using conventional chromatography techniques.

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General info

Function

Essential for sperm motility and male fertility (PubMed : 26677959). Not required for the completion of spermatogenesis (By similarity).

Sequence similarities

Belongs to the phosphoglycerate kinase family.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for sperm motility and male fertility (PubMed : 26677959). Not required for the completion of spermatogenesis (By similarity).
See full target information PGK2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 19:e0310530 PubMed39509399

2024

Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyao Ruan,Chen Wang,Jiawen Gu,Zhihui Zhu

Frontiers in pharmacology 11:379 PubMed32292350

2020

Esculetin Inhibits Cancer Cell Glycolysis by Binding Tumor PGK2, GPD2, and GPI.

Applications

Unspecified application

Species

Unspecified reactive species

Song-Tao Wu,Bo Liu,Zhong-Zhu Ai,Zong-Chao Hong,Peng-Tao You,He-Zhen Wu,Yan-Fang Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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