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AB101059

Recombinant Human PGLS protein

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Recombinant Human PGLS protein is a Human Full Length protein, in the 1 to 258 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, ELISA.

View Alternative Names

6-phosphogluconolactonase, 6PGL, PGLS

1 Images
SDS-PAGE - Recombinant Human PGLS protein (AB101059)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human PGLS protein (AB101059)

15% SDS-PAGE showing ab10105 (3 μg)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

ELISA, SDS-PAGE

applications

Biologically active

No

Accession

O95336

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGSLVSMLARELPAAVAPAGPASLARWTLGFCDERLVPFDHAESTYGLYRTHLLSRLPIPESQVITINPELPVEEAAEDYAKKLRQAFQGDSIPVFDLLILGVGPDGHTCSLFPDHPLLQEREKIVAPISDSPKPPPQRVTLTLPVLNAARTVIFVATGEGKAAVLKRILEDQEENPLPAALVQPHTGKLCWFLDEAAARLLTVPFEKHSTL","proteinLength":"Full Length","predictedMolecularWeight":"29.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":258,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95336","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGLS also known as 6-phosphogluconolactonase operates in the pentose phosphate pathway by catalyzing the hydrolysis of 6-phosphogluconolactone into 6-phosphogluconate. This enzyme has an approximate mass of 27 kDa. PGLS expresses widely in various tissues but it is especially abundant in liver and adipose tissues where the pentose phosphate pathway activity is high.
Biological function summary

6-phosphogluconolactonase plays a role in cellular metabolism by ensuring the smooth progression of the pentose phosphate pathway. This pathway is important for the production of NADPH which acts as a reducing agent in several biosynthetic reactions. PGLS does not form part of a multiprotein complex but serves a singular enzymatic role essential for maintaining cellular redox state.

Pathways

The pentose phosphate pathway lies at the center of PGLS's function in its role of maintaining NADPH levels for reductive biosynthesis and protection against oxidative stress. In this pathway PGLS closely associates with glucose-6-phosphate dehydrogenase (G6PD) which provides the substrate for PGLS. Both play a sequential role in the oxidative branch of the pentose phosphate pathway influencing cellular processes such as fatty acid synthesis and ribose-5-phosphate production.

PGLS connects to the metabolic and oxidative balance within cells indirectly affecting conditions like cancer and metabolic disorders. Alterations in its activity or expression could lead to dysregulation of NADPH levels linking it with oxidative stress-related pathologies. PGLS's functional relation to G6PD could influence its involvement in diseases where altered glucose metabolism and the oxidative state are disturbed.
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Specifications

Form

Liquid

Additional notes

ab101059 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Function

Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.

Sequence similarities

Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.

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Product protocols

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Target data

Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.
See full target information PGLS
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Primary Antibodies

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Anti-PGLS antibody

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Proteins & Peptides

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Secondary Antibodies

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