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AB140549

Recombinant Human PGP protein

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(1 Publication)

Recombinant Human PGP protein is a Human Full Length protein, in the 1 to 321 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Glycerol-3-phosphate phosphatase, G3PP, Aspartate-based ubiquitous Mg(2+)-dependent phosphatase, Phosphoglycolate phosphatase, AUM, PGP

1 Images
SDS-PAGE - Recombinant Human PGP protein (AB140549)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PGP protein (AB140549)

15% SDS-PAGE analysis of ab140549 (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

A6NDG6

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMAAAEAGGDDARCVRLSAERAQALLADVDTLLFDCDGVLWRGETAVPGAPEALRALRARGKRLGFITNNSSKTRAAYAEKLRRLGFGGPAGPGASLEVFGTAYCTALYLRQRLAGAPAPKAYVLGSPALAAELEAVGVASVGVGPEPLQGEGPGDWLHAPLEPDVRAVVVGFDPHFSYMKLTKALRYLQQPGCLLVGTNMDNRLPLENGRFIAGTGCLVRAVEMAAQRQADIIGKPSRFIFDCVSQEYGINPERTVMVGDRLDTDILLGATCGLKTILTLTGVSTLGDVKNNQESDCVSKKKMVPDFYVDSIADLLPALQG","proteinLength":"Full Length","predictedMolecularWeight":"36.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":321,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"A6NDG6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PGP also known as P-glycoprotein or Pgp protein functions as an important membrane-associated ATP-dependent efflux transporter. It has a molecular weight of approximately 170 kDa. This protein is widely expressed in cells throughout the body with high levels present in the liver kidney intestine and blood-brain barrier. PGP's mechanical role involves transporting various substrates including drugs metabolic products and xenobiotics across cellular membranes which is important for protecting tissues from toxins.
Biological function summary

PGP plays an important role in the body's defense by contributing to the cellular excretion of potentially harmful substances. It is part of the ATP-binding cassette (ABC) transporters family. This protein helps maintain cellular homeostasis by regulating the intracellular concentration of substrates. PGP products influence pharmacokinetics by affecting drug absorption and excretion which can affect therapy outcomes. The study of PGP alternatives may provide insights into overcoming drug resistance in various conditions.

Pathways

PGP participates in two major biological systems: the metabolic pathway and the detoxification pathway. In these pathways PGP works closely with other proteins such as cytochrome P450 enzymes to metabolize and eliminate substances from cells. Together with multidrug resistance-associated proteins PGP contributes to the intricate network of cellular defense mechanisms. The proper functioning of these pathways is essential for protecting tissues from damage due to toxins and drug accumulation.

PGP's activity is significant in influencing the development of multidrug resistance in cancer treatment and the progression of neurodegenerative diseases like Alzheimer's disease. Altered PGP expression can lead to inefficient drug clearance in cancer making chemotherapy less effective and allowing cancer cells to survive. In Alzheimer's disease overexpression may reduce the clearance of amyloid-beta a protein linked to the condition which leads to its accumulation in the brain. Consequently research into modulating PGP expression and function is critical for improving therapeutic strategies in these disorders.
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Specifications

Form

Liquid

Additional notes

ab140549 is purified using conventional chromatography techniques.

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General info

Function

Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed : 26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity).

Sequence similarities

Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.

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Product protocols

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Target data

Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear (PubMed : 26755581). In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity).
See full target information PGP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:851 PubMed33051436

2020

FBI-1 enhanced the resistance of triple-negative breast cancer cells to chemotherapeutic agents via the miR-30c/PXR axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Yang,Lili Ren,Yanan Wang,Xuebing Bi,Xiaoli Li,Ming Wen,Qian Zhang,Yang Yang,Youchao Jia,Yumiao Li,Aimin Zang,Yaning Wei,Guanghai Dai
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