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AB177709

Recombinant Human Phafin-2 protein (His tag N-Terminus)

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Recombinant Human Phafin-2 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 249 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ZFYVE18, PLEKHF2, Pleckstrin homology domain-containing family F member 2, PH domain-containing family F member 2, Endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains, PH and FYVE domain-containing protein 2, Phafin-2, Zinc finger FYVE domain-containing protein 18, EAPF, Phafin2

1 Images
SDS-PAGE - Recombinant Human Phafin-2 protein (His tag N-Terminus) (AB177709)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Phafin-2 protein (His tag N-Terminus) (AB177709)

15% SDS-PAGE analysis of ab177709 (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9H8W4

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as PLEKHF2.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMVDRLANSEANTRRISIVENCFGAAGQPLTIPGRVLIGEGVLTKLCRKKPKARQFFLFNDILVYGNIVIQKKKYNKQHIIPLENVTIDSIKDEGDLRNGWLIKTPTKSFAVYAATATEKSEWMNHINKCVTDLLSKSGKTPSNEHAAVWVPDSEATVCMRCQKAKFTPVNRRHHCRKCGFVVCGPCSEKRFLLPSQSSKPVRICDFCYDLLSAGDMATCQPARSDSYSQSLKSPLNDMSDDDDDDDSSD","proteinLength":"Full Length","predictedMolecularWeight":"28 kDa","actualMolecularWeight":null,"aminoAcidEnd":249,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H8W4","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phafin-2 also known as PLEKHF2 is a protein that plays a significant role in cellular processes through its mechanical actions. It is a 38 kDa protein that features a PH (pleckstrin homology) domain and a FYVE domain enabling it to bind phosphoinositides. Phafin-2 is expressed primarily in the brain and heart as well as other tissues indicating its importance in various physiological functions. Its ability to bind phosphoinositides points toward roles in membrane trafficking where its interaction with phosphoinositides is critical.
Biological function summary

This protein contributes to autophagy a cellular process important for the degradation and recycling of cellular components. Phafin-2 can localize to autophagosomes by binding to phosphatidylinositol 3-phosphate through its FYVE domain. It does not work alone but associates with other proteins such as PIK3C3 forming complexes that help regulate autophagosome formation and maturation. This involvement in autophagy highlights its role in maintaining cellular homeostasis and responding to cellular stress.

Pathways

The involvement of Phafin-2 in the PI3K-Akt signaling pathway is notable. This pathway regulates key cellular functions including growth survival and metabolism. Through its interaction with PIK3C3 and related proteins Phafin-2 influences the modulation of autophagy within this pathway. Moreover Phafin-2 also plays a role in endosomal signaling pathways further influencing cellular trafficking and communication.

Alterations in Phafin-2 function have been linked to neurodegenerative diseases and certain cancers. In disorders like Alzheimer's disease Phafin-2's role in autophagy highlights its connection to pathogenic protein aggregation. In cancer aberrant signaling or expression levels of Phafin-2 and interaction with proteins such as mTOR suggests its involvement in tumor progression and proliferation. Understanding these relationships provides insight into its potential as a therapeutic target.
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Specifications

Form

Liquid

Additional notes

ab177709 was purified using conventional chromatography techniques.

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General info

Function

May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis (PubMed : 18288467).

Subcellular localisation

Early endosome membrane

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Product protocols

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Target data

May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis (PubMed : 18288467).
See full target information PLEKHF2
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