Recombinant Human PHAP1 protein is a Human Full Length protein, in the 1 to 249 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M E M G R R I H L E L R N R T P S D V K E L V L D N S R S N E G K L E G L T D E F E E L E F L S T I N V G L T S I A N L P K L N K L K K L E L S D N R V S G G L E V L A E K C P N L T H L N L S G N K I K D L S T I E P L K K L E N L K S L D L F N C E V T N L N D Y R E N V F K L L P Q L T Y L D G Y D R D D K E A P D S D A E G Y V E G L D D E E E D E D E E E Y D E D A Q V V E D E E D E D E E E E G E E E D V S G E E E E D E E G Y N D G E V D D E E D E E E L G E E E R G Q K R K R E P E D E G E D D D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription (PubMed:10400610, PubMed:11360199, PubMed:16341127, PubMed:18439902). Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation (PubMed:18439902). In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking (PubMed:11830591). Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition (PubMed:16341127). Participates in other biochemical processes such as regulation of mRNA nuclear-to-cytoplasmic translocation and stability by its association with ELAVL1 (Hu-antigen R) (PubMed:18180367). Plays a role in E4F1-mediated transcriptional repression as well as inhibition of protein phosphatase 2A (PubMed:15642345, PubMed:17557114). (Microbial infection) Plays an essential role in influenza A, B and C viral genome replication (PubMed:30666459, PubMed:32694517, PubMed:33045004, PubMed:33208942). Mechanistically, mediates the assembly of the viral replicase asymmetric dimers composed of PB1, PB2 and PA via its N-terminal region (PubMed:33208942). Also plays an essential role in foamy virus mRNA export from the nucleus (PubMed:21159877).
Alternative names
C15orf1, LANP, MAPM, PHAP1, ANP32A, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, Mapmodulin, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I, pp32, PHAPI
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Recombinant Human PHAP1 protein is a Human Full Length protein, in the 1 to 249 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P39687-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M E M G R R I H L E L R N R T P S D V K E L V L D N S R S N E G K L E G L T D E F E E L E F L S T I N V G L T S I A N L P K L N K L K K L E L S D N R V S G G L E V L A E K C P N L T H L N L S G N K I K D L S T I E P L K K L E N L K S L D L F N C E V T N L N D Y R E N V F K L L P Q L T Y L D G Y D R D D K E A P D S D A E G Y V E G L D D E E E D E D E E E Y D E D A Q V V E D E E D E D E E E E G E E E D V S G E E E E D E E G Y N D G E V D D E E D E E E L G E E E R G Q K R K R E P E D E G E D D D
- Accession
- P39687
- Protein length
- Full Length
- Predicted molecular weight
- 30.7 kDa
- Amino acids
- 1 to 249
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab101155 was purified using conventional chromatography techniques.
General info
- Function
Multifunctional protein that is involved in the regulation of many processes including tumor suppression, apoptosis, cell cycle progression or transcription (PubMed:10400610, PubMed:11360199, PubMed:16341127, PubMed:18439902). Promotes apoptosis by favouring the activation of caspase-9/CASP9 and allowing apoptosome formation (PubMed:18439902). In addition, plays a role in the modulation of histone acetylation and transcription as part of the INHAT (inhibitor of histone acetyltransferases) complex. Inhibits the histone-acetyltranferase activity of EP300/CREBBP (CREB-binding protein) and EP300/CREBBP-associated factor by histone masking (PubMed:11830591). Preferentially binds to unmodified histone H3 and sterically inhibiting its acetylation and phosphorylation leading to cell growth inhibition (PubMed:16341127). Participates in other biochemical processes such as regulation of mRNA nuclear-to-cytoplasmic translocation and stability by its association with ELAVL1 (Hu-antigen R) (PubMed:18180367). Plays a role in E4F1-mediated transcriptional repression as well as inhibition of protein phosphatase 2A (PubMed:15642345, PubMed:17557114).
- Sequence similarities
Belongs to the ANP32 family.
- Post-translational modifications
Phosphorylated on serine residues, at least in part by casein kinase 2/CK2.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PHAP1 also known as putative HLA-associated protein 1 or ANP32A is a multifunctional phosphoprotein with a molecular weight of approximately 32 kDa. It exists within the nucleus and cytoplasm indicating its role in various cellular processes. PHAP1 expression is found across diverse tissues showing its widespread functional requirements in different biological contexts.
- Biological function summary
PHAP1 plays an important role in regulating apoptosis and cell proliferation. It interacts with components of the INHAT complex which inhibits histone acetylation impacting chromatin remodeling and gene expression. By doing so it influences many cellular events such as cell cycle progression and differentiation. PHAP1's involvement in regulating cell death makes it a target for studying mechanisms underlying cell survival.
- Pathways
PHAP1 is associated with apoptosis and cell signaling processes. It is intricately linked with the p38 MAPK signaling pathway important in stress response and inflammation. In these contexts PHAP1 interacts with proteins such as caspases and protein phosphatase 2A which are important for cellular responses to external and internal stimuli.
- Associated diseases and disorders
PHAP1 shows connections with cancer and neurodegenerative diseases. Altered expression levels of PHAP1 have been observed in certain types of cancers possibly affecting tumor growth and treatment responses. In neurodegeneration relations have been reported with tau proteins suggesting PHAP1's role in tauopathies potentially influencing disease progression and providing insights into therapeutic targets.
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SDS-PAGE - Recombinant Human PHAP1 protein (ab101155)
15% SDS-PAGE (3 μg)
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