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AB133003

Recombinant Human PHAPI2 / APRIL protein (GST tag N-Terminus)

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Recombinant Human PHAPI2 / APRIL protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 251 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

APRIL, PHAPI2, ANP32B, Acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, Putative HLA-DR-associated protein I-2, Silver-stainable protein SSP29

1 Images
SDS-PAGE - Recombinant Human PHAPI2 / APRIL protein (GST tag N-Terminus) (AB133003)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PHAPI2 / APRIL protein (GST tag N-Terminus) (AB133003)

12.5% SDS-PAGE analysis of ab133003 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

Q92688

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB200836

Anti-PHAPI2 / APRIL antibody [EPR14588]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHAPI2 / APRIL antibody [EPR14588] (AB200836)

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Cell Lines & Lysates

AB266818

Human ANP32B (PHAPI2/APRIL) knockout HEK-293T cell line

Advanced Validation

Western blot - Human ANP32B (PHAPI2/APRIL) knockout HEK-293T cell line (AB266818)

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Cell Lines & Lysates

AB257831

Human ANP32B (PHAPI2/APRIL) knockout HEK-293T cell lysate

Western blot - Human ANP32B (PHAPI2/APRIL) knockout HEK-293T cell lysate (AB257831)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIEGLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLELSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDISTLEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLPQLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGEDEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVSEEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETDDEGEDD","proteinLength":"Full Length","predictedMolecularWeight":"55.2 kDa","actualMolecularWeight":null,"aminoAcidEnd":251,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q92688","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHAPI2 also known as APRIL is a protein with various roles in cellular mechanisms. The protein has a molecular mass of around 32 kDa and it is found in many tissues including the spleen liver and lymph nodes. PHAPI2 acts as part of the tumor necrosis factor ligand superfamily where it influences cell proliferation and survival. This protein interacts with receptors such as BCMA and TACI impacting immune system functions.
Biological function summary

PHAPI2 regulates immune system processes by playing a role in B cell development and activity. It functions as part of larger protein complexes binding with proteins such as BCMA to influence signaling pathways. The interactions within these complexes help modulate processes critical for proper immune response and maintenance of immune system homeostasis.

Pathways

PHAPI2 is deeply involved in both the NF-kB signaling pathway and the PI3K/AKT pathway. These pathways are essential for cellular growth survival and proliferation. Through its involvement PHAPI2 also connects with proteins like NF-kB and AKT1 which are key regulatory proteins within these pathways. This connection enables PHAPI2 to act as a node integrating signals for various cellular responses.

PHAPI2 associations include autoimmune diseases and cancer. Aberrant expression or mutations in PHAPI2 are linked to conditions like systemic lupus erythematosus and certain B cell malignancies. Through these disorders PHAPI2 interacts with proteins such as BAFF and CD40 which are important for disease progression and immune dysregulation. This makes PHAPI2 a potential therapeutic target for intervention in these conditions.
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Specifications

Form

Liquid

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General info

Function

Multifunctional protein that is involved in the regulation of many processes including cell proliferation, apoptosis, cell cycle progression or transcription (PubMed : 18039846, PubMed : 20015864). Regulates the proliferation of neuronal stem cells, differentiation of leukemic cells and progression from G1 to S phase of the cell cycle. As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis (PubMed : 20015864). Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes (PubMed : 18039846, PubMed : 20538007). Also plays an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1 (PubMed : 17178712). Participates in the regulation of adequate adaptive immune responses by acting on mRNA expression and cell proliferation (By similarity).. (Microbial infection) Plays an essential role in influenza A and B viral genome replication (PubMed : 31217244, PubMed : 33045004). Also plays a role in foamy virus mRNA export from the nucleus to the cytoplasm (PubMed : 21159877).

Sequence similarities

Belongs to the ANP32 family.

Post-translational modifications

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).. Directly cleaved by caspase-3/CASP3.

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Product protocols

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Target data

Multifunctional protein that is involved in the regulation of many processes including cell proliferation, apoptosis, cell cycle progression or transcription (PubMed : 18039846, PubMed : 20015864). Regulates the proliferation of neuronal stem cells, differentiation of leukemic cells and progression from G1 to S phase of the cell cycle. As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis (PubMed : 20015864). Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes (PubMed : 18039846, PubMed : 20538007). Also plays an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1 (PubMed : 17178712). Participates in the regulation of adequate adaptive immune responses by acting on mRNA expression and cell proliferation (By similarity).. (Microbial infection) Plays an essential role in influenza A and B viral genome replication (PubMed : 31217244, PubMed : 33045004). Also plays a role in foamy virus mRNA export from the nucleus to the cytoplasm (PubMed : 21159877).
See full target information ANP32B
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