Recombinant Human PHAPI2 / APRIL protein is a Human Full Length protein, in the 1 to 251 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M D M K R R I H L E L R N R T P A A V R E L V L D N C K S N D G K I E G L T A E F V N L E F L S L I N V G L I S V S N L P K L P K L K K L E L S E N R I F G G L D M L A E K L P N L T H L N L S G N K L K D I S T L E P L K K L E C L K S L D L F N C E V T N L N D Y R E S V F K L L P Q L T Y L D G Y D R E D Q E A P D S D A E V D G V D E E E E D E E G E D E E D E D D E D G E E E E F D E E D D E D E D V E G D E D D D E V S E E E E E F G L D E E D E D E D E D E E E E E G G K G E K R K R E T D D E G E D D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional protein that is involved in the regulation of many processes including cell proliferation, apoptosis, cell cycle progression or transcription (PubMed:18039846, PubMed:20015864). Regulates the proliferation of neuronal stem cells, differentiation of leukemic cells and progression from G1 to S phase of the cell cycle. As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis (PubMed:20015864). Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes (PubMed:18039846, PubMed:20538007). Also plays an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1 (PubMed:17178712). Participates in the regulation of adequate adaptive immune responses by acting on mRNA expression and cell proliferation (By similarity). (Microbial infection) Plays an essential role in influenza A and B viral genome replication (PubMed:31217244, PubMed:33045004). Also plays a role in foamy virus mRNA export from the nucleus to the cytoplasm (PubMed:21159877).
Alternative names
APRIL, PHAPI2, ANP32B, Acidic leucine-rich nuclear phosphoprotein 32 family member B, Acidic protein rich in leucines, Putative HLA-DR-associated protein I-2, Silver-stainable protein SSP29
Recommended products
Recombinant Human PHAPI2 / APRIL protein is a Human Full Length protein, in the 1 to 251 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- Q92688-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M D M K R R I H L E L R N R T P A A V R E L V L D N C K S N D G K I E G L T A E F V N L E F L S L I N V G L I S V S N L P K L P K L K K L E L S E N R I F G G L D M L A E K L P N L T H L N L S G N K L K D I S T L E P L K K L E C L K S L D L F N C E V T N L N D Y R E S V F K L L P Q L T Y L D G Y D R E D Q E A P D S D A E V D G V D E E E E D E E G E D E E D E D D E D G E E E E F D E E D D E D E D V E G D E D D D E V S E E E E E F G L D E E D E D E D E D E E E E E G G K G E K R K R E T D D E G E D D
- Accession
- Q92688
- Protein length
- Full Length
- Predicted molecular weight
- 55.2 kDa
- Amino acids
- 1 to 251
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Multifunctional protein that is involved in the regulation of many processes including cell proliferation, apoptosis, cell cycle progression or transcription (PubMed:18039846, PubMed:20015864). Regulates the proliferation of neuronal stem cells, differentiation of leukemic cells and progression from G1 to S phase of the cell cycle. As negative regulator of caspase-3-dependent apoptosis, may act as an antagonist of ANP32A in regulating tissue homeostasis (PubMed:20015864). Exhibits histone chaperone properties, able to recruit histones to certain promoters, thus regulating the transcription of specific genes (PubMed:18039846, PubMed:20538007). Also plays an essential role in the nucleocytoplasmic transport of specific mRNAs via the uncommon nuclear mRNA export receptor XPO1/CRM1 (PubMed:17178712). Participates in the regulation of adequate adaptive immune responses by acting on mRNA expression and cell proliferation (By similarity).
- Sequence similarities
Belongs to the ANP32 family.
- Post-translational modifications
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PHAPI2 also known as APRIL is a protein with various roles in cellular mechanisms. The protein has a molecular mass of around 32 kDa and it is found in many tissues including the spleen liver and lymph nodes. PHAPI2 acts as part of the tumor necrosis factor ligand superfamily where it influences cell proliferation and survival. This protein interacts with receptors such as BCMA and TACI impacting immune system functions.
- Biological function summary
PHAPI2 regulates immune system processes by playing a role in B cell development and activity. It functions as part of larger protein complexes binding with proteins such as BCMA to influence signaling pathways. The interactions within these complexes help modulate processes critical for proper immune response and maintenance of immune system homeostasis.
- Pathways
PHAPI2 is deeply involved in both the NF-kB signaling pathway and the PI3K/AKT pathway. These pathways are essential for cellular growth survival and proliferation. Through its involvement PHAPI2 also connects with proteins like NF-kB and AKT1 which are key regulatory proteins within these pathways. This connection enables PHAPI2 to act as a node integrating signals for various cellular responses.
- Associated diseases and disorders
PHAPI2 associations include autoimmune diseases and cancer. Aberrant expression or mutations in PHAPI2 are linked to conditions like systemic lupus erythematosus and certain B cell malignancies. Through these disorders PHAPI2 interacts with proteins such as BAFF and CD40 which are important for disease progression and immune dysregulation. This makes PHAPI2 a potential therapeutic target for intervention in these conditions.
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1 product image
SDS-PAGE - Recombinant Human PHAPI2 / APRIL protein (ab133003)
12.5% SDS-PAGE analysis of ab133003 stained with Coomassie Blue.
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