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AB161607

Recombinant Human PHF15 protein (GST tag N-Terminus)

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Recombinant Human PHF15 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 246 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

KIAA0239, PHF15, JADE2, E3 ubiquitin-protein ligase Jade-2, Jade family PHD finger protein 2, PHD finger protein 15

1 Images
SDS-PAGE - Recombinant Human PHF15 protein (GST tag N-Terminus) (AB161607)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PHF15 protein (GST tag N-Terminus) (AB161607)

ab161607 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q9NQC1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAMSEWPLNNGHREDPAPGLLSEELLQDEETLLSFMRDPSLRPGDPARKARGRTRLPAKKKPPPPPPQDGPGSRTTPDKAPKKTWGQDAGSGKGGQGPPTRKPPRRTSSHLPSSPAAGDCPILATPESPPPLAPETPDEAASVAADSDVQVPGPAASPKPLGRLRPPRESKVTRRLPGARPDAGMGPPSAVAERPKISLHFDTETDGYFSDGEMSDSDVEAEDGGVQRGPREAGAEEVVRMGVLAS","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":246,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9NQC1","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHF15 also known as Jade-1 is a protein of approximately 62 kilodaltons and is involved in chromatin remodeling. PHF15 contains a plant homeodomain (PHD) finger that likely contributes to its function in recognizing methylated histones. The protein is mainly expressed in the nucleus of kidney cells but can also be detected in other tissues. PHF15 has an important role in regulating gene expression by influencing the chromatin structure availability for transcriptional machinery.
Biological function summary

The role of PHF15 involves the regulation of gene expression during critical cellular processes. It interacts within the chromatin remodeling complex facilitating changes in the chromatin structure that are necessary for transcriptional activation or repression. The modulation of chromatin architecture by PHF15 impacts various biological activities that are essential for maintaining cell identity and function. Its precise mechanistic involvement in chromatin modifications highlights its biological importance.

Pathways

The activity of PHF15 features in the Wnt signaling pathway and the regulation of the cell cycle. These pathways involve interactions with other proteins like β-catenin and cyclin D1 where PHF15 exerts regulatory effects. In the Wnt signaling pathway PHF15 modulates gene expression impacting various cellular outcomes such as proliferation and differentiation. Through its integration in these pathways PHF15 has a fundamental influence on cellular fate and behavior.

Defects in PHF15 function connect to clear cell renal cell carcinoma. Alterations in its interaction with proteins such as von Hippel-Lindau (VHL) can disrupt normal cellular regulation contributing to disease progression. Furthermore PHF15 interacts with PBRM1 and changes in their regulation can play a role in tumorigenesis. Understanding the role of PHF15 in these contexts is significant for unraveling its contributions to disease mechanisms and potential therapeutic strategies.
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Specifications

Form

Liquid

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General info

Function

Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (PubMed : 16387653). Acts as an E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed : 25018020). Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (By similarity).

Sequence similarities

Belongs to the JADE family.

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Product protocols

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Target data

Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (PubMed : 16387653). Acts as an E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed : 25018020). Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (By similarity).
See full target information JADE2
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