Recombinant Human PHF15 protein is a Human Full Length protein, in the 1 to 246 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A M S E W P L N N G H R E D P A P G L L S E E L L Q D E E T L L S F M R D P S L R P G D P A R K A R G R T R L P A K K K P P P P P P Q D G P G S R T T P D K A P K K T W G Q D A G S G K G G Q G P P T R K P P R R T S S H L P S S P A A G D C P I L A T P E S P P P L A P E T P D E A A S V A A D S D V Q V P G P A A S P K P L G R L R P P R E S K V T R R L P G A R P D A G M G P P S A V A E R P K I S L H F D T E T D G Y F S D G E M S D S D V E A E D G G V Q R G P R E A G A E E V V R M G V L A S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (PubMed:16387653). Acts as an E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed:25018020). Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (By similarity).
Alternative names
KIAA0239, PHF15, JADE2, E3 ubiquitin-protein ligase Jade-2, Jade family PHD finger protein 2, PHD finger protein 15
Recommended products
Recombinant Human PHF15 protein is a Human Full Length protein, in the 1 to 246 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9NQC1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A M S E W P L N N G H R E D P A P G L L S E E L L Q D E E T L L S F M R D P S L R P G D P A R K A R G R T R L P A K K K P P P P P P Q D G P G S R T T P D K A P K K T W G Q D A G S G K G G Q G P P T R K P P R R T S S H L P S S P A A G D C P I L A T P E S P P P L A P E T P D E A A S V A A D S D V Q V P G P A A S P K P L G R L R P P R E S K V T R R L P G A R P D A G M G P P S A V A E R P K I S L H F D T E T D G Y F S D G E M S D S D V E A E D G G V Q R G P R E A G A E E V V R M G V L A S
- Accession
- Q9NQC1
- Protein length
- Full Length
- Amino acids
- 1 to 246
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (PubMed:16387653). Acts as an E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed:25018020). Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (By similarity).
- Sequence similarities
Belongs to the JADE family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PHF15 also known as Jade-1 is a protein of approximately 62 kilodaltons and is involved in chromatin remodeling. PHF15 contains a plant homeodomain (PHD) finger that likely contributes to its function in recognizing methylated histones. The protein is mainly expressed in the nucleus of kidney cells but can also be detected in other tissues. PHF15 has an important role in regulating gene expression by influencing the chromatin structure availability for transcriptional machinery.
- Biological function summary
The role of PHF15 involves the regulation of gene expression during critical cellular processes. It interacts within the chromatin remodeling complex facilitating changes in the chromatin structure that are necessary for transcriptional activation or repression. The modulation of chromatin architecture by PHF15 impacts various biological activities that are essential for maintaining cell identity and function. Its precise mechanistic involvement in chromatin modifications highlights its biological importance.
- Pathways
The activity of PHF15 features in the Wnt signaling pathway and the regulation of the cell cycle. These pathways involve interactions with other proteins like β-catenin and cyclin D1 where PHF15 exerts regulatory effects. In the Wnt signaling pathway PHF15 modulates gene expression impacting various cellular outcomes such as proliferation and differentiation. Through its integration in these pathways PHF15 has a fundamental influence on cellular fate and behavior.
- Associated diseases and disorders
Defects in PHF15 function connect to clear cell renal cell carcinoma. Alterations in its interaction with proteins such as von Hippel-Lindau (VHL) can disrupt normal cellular regulation contributing to disease progression. Furthermore PHF15 interacts with PBRM1 and changes in their regulation can play a role in tumorigenesis. Understanding the role of PHF15 in these contexts is significant for unraveling its contributions to disease mechanisms and potential therapeutic strategies.
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1 product image
SDS-PAGE - Recombinant Human PHF15 protein (ab161607)
ab161607 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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